FastQCFastQC Report
Thu 26 May 2016
SRR1926617_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926617_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766097
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTGG350.0060806218.971
TATAAGA500.002135146416.3216022
CTGACCG350.002727140715.7356739
GTCCTAC4000.015.270851
GTCCTAA2050.014.8983911
TCCTACT550.00402990314.8378212
TCCTACA4900.014.1564932
GTCCTAT852.9050863E-514.0601171
CCTATAC550.001089492913.8724793
TCCTAAA2150.013.2850262
ATTTAGA802.7391815E-413.2789991
TATACCT550.001569973813.2168055
TCCAACG606.855911E-512.834167518
CCTAGCC600.002098671612.7164393
GTCTTGG1051.4970288E-512.6466671
GTCCAAG958.253994E-512.5801061
GTGTAGG750.002544161612.3937331
CCTACAC1151.4794023E-712.3215183
CCTACAG5400.012.1108953
GTGTAAC1102.4131043E-512.0718191
Produced by FastQC (version 0.11.5)