FastQCFastQC Report
Thu 26 May 2016
SRR1926617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766097
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13640.17804533890616983No Hit
GTCCTACAGTGGACATTTCTAAATT9690.1264852884164799No Hit
CTGTAGGACGTGGAATATGGCAAGA9570.12491890713578047No Hit
GTCCTACAGTGTGCATTTCTCATTT8420.10990775319574414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCC400.00527705614.2495098
TAGGACC3550.013.4432024
TCCAACG854.0780324E-612.25517318
GTCCTAC5900.011.9626281
TGGCGAG5550.011.94402718
TGTAGGA7300.011.6389542
TTAGGAC3450.011.6218983
CTGTAGG7250.011.5768811
GTAGGAC7100.011.56343653
GTAGAAC2250.011.4558713
CTAACAC751.9792028E-411.455873
CTAGCAC751.9792028E-411.455873
GGACCTG5100.011.4154276
GTACTGG1451.1350494E-911.1822151
ATGGCGA6100.011.17905417
AGGACCT5050.011.1512025
TAGAAGC600.005669473711.1354534
TAGGGCC600.005669473711.1354534
GGCGAGG2300.011.11539619
TATGGCG6050.011.11489616
Produced by FastQC (version 0.11.5)