##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926617_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 766097 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.43194660728341 31.0 28.0 31.0 22.0 34.0 2 28.88331373181203 31.0 28.0 33.0 23.0 34.0 3 29.23897757072538 31.0 29.0 33.0 25.0 34.0 4 32.995638933450984 35.0 33.0 37.0 27.0 37.0 5 32.72675131217065 35.0 33.0 37.0 26.0 37.0 6 32.7940430519895 35.0 33.0 37.0 26.0 37.0 7 32.639600468348 35.0 33.0 37.0 25.0 37.0 8 32.59649626613862 35.0 33.0 37.0 25.0 37.0 9 33.86701031331542 38.0 34.0 39.0 24.0 39.0 10 33.77027843732582 37.0 33.0 39.0 23.0 39.0 11 33.98847404440952 37.0 34.0 39.0 25.0 39.0 12 33.79685601170609 37.0 34.0 39.0 25.0 39.0 13 33.93079466438323 37.0 34.0 39.0 25.0 39.0 14 34.593364808894954 39.0 34.0 40.0 23.0 41.0 15 34.716909216456926 39.0 34.0 40.0 25.0 41.0 16 34.69661413633 39.0 34.0 40.0 24.0 41.0 17 34.61164447844072 39.0 34.0 40.0 24.0 41.0 18 34.57519348072111 39.0 34.0 40.0 23.0 41.0 19 34.63113678816129 39.0 34.0 40.0 23.0 41.0 20 34.609570328561524 39.0 34.0 40.0 22.0 41.0 21 34.5319378616546 39.0 34.0 40.0 21.0 41.0 22 34.47360712807908 39.0 34.0 40.0 19.0 41.0 23 34.138665208191654 39.0 34.0 40.0 18.0 41.0 24 34.215092866830176 39.0 34.0 41.0 18.0 41.0 25 34.003998188218986 39.0 34.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8214320863808631 1101 2 1.0201530267350627 1101 3 0.8071470132423109 1101 4 0.790116404727172 1101 5 0.515325898293348 1101 6 0.408374630616791 1101 7 0.5839817696804559 1101 8 0.6367113306936361 1101 9 0.7275361141472487 1101 10 0.9885078059578376 1101 11 0.7252007148273734 1101 12 0.8452761399611362 1101 13 0.7034156335737265 1101 14 1.0763197529336779 1101 15 0.9682299398279852 1101 16 0.7999353328827752 1101 17 0.7025346496535931 1101 18 0.77461998199432 1101 19 0.8474719304632785 1101 20 0.7663408489167267 1101 21 0.7156857502552398 1101 22 0.6840797658575184 1101 23 0.9101968951301771 1101 24 0.9369907927750916 1101 25 1.023104965980501 1102 1 1.0035370841242823 1102 2 1.109254060100671 1102 3 1.1045378551269884 1102 4 0.8548167032738476 1102 5 1.2134261328506 1102 6 0.8370921088402596 1102 7 1.2207867057651924 1102 8 1.5081360276443974 1102 9 1.5587830815359496 1102 10 1.5701451723905109 1102 11 1.3147806094355232 1102 12 1.316086792716817 1102 13 1.2342809951669338 1102 14 1.5793497156630139 1102 15 1.4233250446371528 1102 16 1.2426287790521968 1102 17 1.5527432509338084 1102 18 1.4041773743617867 1102 19 1.5567756111263975 1102 20 1.6733051017130833 1102 21 1.4688836322477243 1102 22 1.3797613273689393 1102 23 1.8092715911274482 1102 24 1.6815426867136836 1102 25 1.764701057116696 1103 1 0.5201856459719174 1103 2 0.4011961891175524 1103 3 0.3575295437519266 1103 4 0.14484492043062858 1103 5 0.47779969329781125 1103 6 0.1983584869281927 1103 7 0.43075134919544666 1103 8 0.3810352553795866 1103 9 0.5158746604336031 1103 10 0.8018196722746325 1103 11 0.5546709260849667 1103 12 0.49260548315076846 1103 13 0.22929438980403205 1103 14 0.7176741942936502 1103 15 0.640788817608211 1103 16 0.6292790653426579 1103 17 0.7695293288588516 1103 18 0.7597003693125686 1103 19 0.602935760241273 1103 20 0.6583018999609607 1103 21 0.7776956449141466 1103 22 0.6382597001319681 1103 23 1.1189677926302437 1103 24 1.023395353394264 1103 25 0.8610172298641317 1104 1 0.9045827109033873 1104 2 0.8100585978527839 1104 3 0.626005461014504 1104 4 0.3606596037151846 1104 5 0.4891421211794622 1104 6 0.12609417621531804 1104 7 0.35803359894243414 1104 8 0.502686912929164 1104 9 0.8795508799176588 1104 10 0.824332811289004 1104 11 0.601905910796809 1104 12 0.7042694506447873 1104 13 0.41360012288966175 1104 14 0.793673880910589 1104 15 0.9198322909228978 1104 16 0.6098643404167916 1104 17 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2202 25 0.2998481123490748 2203 1 0.33399219630881305 2203 2 0.37408382571683063 2203 3 0.02705384908227515 2203 4 -0.27235750565408523 2203 5 0.0372926652401091 2203 6 0.17372972070833015 2203 7 0.44839768885993436 2203 8 0.48323295476618 2203 9 0.7087072985854874 2203 10 0.4771147393466819 2203 11 0.37470627157015457 2203 12 0.6066357122076553 2203 13 0.36467023835682966 2203 14 0.8071164094763716 2203 15 0.6159677691938512 2203 16 0.4080523738189754 2203 17 0.5634217575896017 2203 18 0.6299735994410653 2203 19 0.6408997271320018 2203 20 0.6900921734666028 2203 21 0.6338725812675889 2203 22 0.40512873185049614 2203 23 0.8461285422667686 2203 24 0.7688739954342694 2203 25 0.7780552116242987 2204 1 0.6264405344552415 2204 2 0.6143370937412094 2204 3 0.49340498946860833 2204 4 0.3646476668556602 2204 5 0.5251855294702992 2204 6 0.28232712787608705 2204 7 0.6827779855385927 2204 8 0.7658759549005367 2204 9 1.177579763417647 2204 10 1.0329535027565058 2204 11 0.8194645308050283 2204 12 0.7752299599900425 2204 13 0.9082008908598596 2204 14 1.2538967877818479 2204 15 1.002297637134916 2204 16 0.9582795741032086 2204 17 1.1589124939006652 2204 18 1.1558875865797802 2204 19 0.9771139851795851 2204 20 0.972171881049178 2204 21 1.1235767514876258 2204 22 1.2427829837816802 2204 23 1.205239911725407 2204 24 1.3551215619486072 2204 25 1.091732145918641 2205 1 0.5561120049603865 2205 2 0.8496750465717895 2205 3 0.917448308091199 2205 4 0.720619461080716 2205 5 0.7562141796486088 2205 6 0.630758164672784 2205 7 0.502296954146793 2205 8 0.7524649998092983 2205 9 1.1318500828606375 2205 10 1.0738882224612212 2205 11 0.9180688118037281 2205 12 1.0622633560411714 2205 13 0.9537686853501981 2205 14 1.3899536714123997 2205 15 1.2578311378897737 2205 16 1.0444724159191097 2205 17 1.154754633052704 2205 18 1.2453740265328435 2205 19 1.0737192343614055 2205 20 1.0676099839115878 2205 21 0.9798245353457062 2205 22 1.009971498017471 2205 23 1.109139642628989 2205 24 1.0342967994380743 2205 25 1.1056162260950657 2206 1 0.3061574058157923 2206 2 0.5196024810820035 2206 3 0.6605978375157591 2206 4 0.533632686767227 2206 5 0.5845468427186162 2206 6 0.5202765109181726 2206 7 0.6909596397022426 2206 8 0.64100754382536 2206 9 1.02410563276743 2206 10 0.7830414901846297 2206 11 0.7063652553612343 2206 12 0.6405195079203096 2206 13 0.4808298715392283 2206 14 0.8523341938131068 2206 15 0.6917540500195187 2206 16 0.66952862951306 2206 17 0.5862155288786113 2206 18 0.5772697082665275 2206 19 0.43390862801337704 2206 20 0.5488140297727782 2206 21 0.8222706676733935 2206 22 0.7364408290440849 2206 23 0.9839900980286558 2206 24 0.9383550541735488 2206 25 1.0913226709930939 2207 1 -1.3593412971047947 2207 2 -0.013292062046524222 2207 3 0.016815442259364488 2207 4 0.07740188278106075 2207 5 0.01229012760396131 2207 6 -0.06900418112826401 2207 7 0.2734650590066394 2207 8 0.24462312096224537 2207 9 0.5327399680586566 2207 10 0.03351793207496456 2207 11 0.22398269054534126 2207 12 0.15971323726476072 2207 13 0.010689096540630771 2207 14 -5.461607252316217 2207 15 -1.9660246189891666 2207 16 -0.938402084616726 2207 17 -0.7525161254433783 2207 18 -0.12318261962296617 2207 19 -0.008545212712377293 2207 20 0.07240970651317014 2207 21 0.2955107263592538 2207 22 0.12532351316082924 2207 23 0.5723437971163818 2207 24 0.2115468848577251 2207 25 0.23243069664023608 2208 1 0.49139766341364677 2208 2 0.8017315671234115 2208 3 0.6524976594538892 2208 4 0.6336762577766422 2208 5 0.7934242469946255 2208 6 0.7041340815699542 2208 7 0.9932996295776491 2208 8 0.9674637096269052 2208 9 1.255093435403353 2208 10 1.1884805625502821 2208 11 0.9947358255370062 2208 12 0.9586896262672298 2208 13 0.7212400938521242 2208 14 1.2406727783345701 2208 15 1.1092217432493925 2208 16 1.2790004128769965 2208 17 1.0686911681509486 2208 18 1.1987071486082428 2208 19 1.0608400452297175 2208 20 1.0171676248847632 2208 21 1.4009777924149276 2208 22 1.4027045268375815 2208 23 1.556772572912358 2208 24 1.4974209723255356 2208 25 1.4815874467283265 2209 1 -0.28999632872857717 2209 2 -0.3992550356233977 2209 3 0.18181270868696942 2209 4 0.4969158885062299 2209 5 0.5741640364221396 2209 6 0.47614364856330127 2209 7 0.514202901483074 2209 8 0.5965465090269504 2209 9 0.7537569627363823 2209 10 0.41447376240947165 2209 11 0.412611875369727 2209 12 0.30469990779473477 2209 13 0.6313876855519851 2209 14 0.6150792349533702 2209 15 0.5989646106298565 2209 16 0.6589390007119746 2209 17 0.7202655303295344 2209 18 0.6924381868284755 2209 19 0.5992320915516771 2209 20 0.45579297028228893 2209 21 0.47831798359740674 2209 22 0.5896548876983161 2209 23 0.8369245242149148 2209 24 0.8021645453216024 2209 25 0.6813086629892737 2210 1 -0.16610776646164993 2210 2 -0.45634003401869094 2210 3 0.3859383700964436 2210 4 0.4895563856532519 2210 5 0.7348938518949311 2210 6 0.5981429157940283 2210 7 0.8499021138981178 2210 8 1.037151583312145 2210 9 1.3614067331069393 2210 10 1.2167061525153855 2210 11 0.8685528448405151 2210 12 0.6798453965774058 2210 13 0.9404118243350723 2210 14 1.1216596734220587 2210 15 0.9718129529523978 2210 16 0.9505283431793572 2210 17 1.2546247406428321 2210 18 0.986358758047686 2210 19 1.0655744084090628 2210 20 1.12789803462681 2210 21 1.3610334333842502 2210 22 1.195534651053606 2210 23 1.4611774829838637 2210 24 1.1854801274613394 2210 25 1.4242333212432925 2211 1 -1.1596744516577928 2211 2 -0.24704660481025797 2211 3 0.313562233671675 2211 4 0.5562075114684006 2211 5 0.8306774993327863 2211 6 0.49528997409015574 2211 7 0.6789288381854846 2211 8 0.6355711982576651 2211 9 1.0578956961137393 2211 10 1.027102982181674 2211 11 0.6639981798953016 2211 12 0.8630576905864089 2211 13 0.747107540439579 2211 14 0.8960298206417363 2211 15 0.9074845867142969 2211 16 0.9323419605516392 2211 17 0.6533193091152256 2211 18 0.9037902139266123 2211 19 0.9431662936936718 2211 20 0.986666362371416 2211 21 0.7269391960515392 2211 22 0.6559060774049144 2211 23 1.0048538507282316 2211 24 0.9856349382514722 2211 25 1.022033693028284 2212 1 0.8524832101588835 2212 2 -0.02251096216006232 2212 3 0.29408756080720977 2212 4 0.4722978040596999 2212 5 0.4341510260289567 2212 6 0.42534441708419735 2212 7 0.4536991269562165 2212 8 0.7766339509718492 2212 9 0.6439589263980494 2212 10 0.29984009574785375 2212 11 0.3210417024525256 2212 12 0.7797656405370574 2212 13 0.8283874283697941 2212 14 0.5292693379775315 2212 15 0.7869853667454976 2212 16 0.12574767229031636 2212 17 0.658781437804457 2212 18 0.7886576086224011 2212 19 0.5627110101762085 2212 20 0.5952371541826835 2212 21 0.7079471873916034 2212 22 0.8146542069219294 2212 23 1.0903681937509333 2212 24 0.6615361130739785 2212 25 0.20398154237082622 2213 1 0.1723216529466356 2213 2 0.05215622642417728 2213 3 -0.4886009077798761 2213 4 -1.0981550187433555 2213 5 -1.9587283576338415 2213 6 -0.6625932374893964 2213 7 -0.4451838508207686 2213 8 -0.4111830694718961 2213 9 -0.956662392748612 2213 10 -1.0247653523598999 2213 11 -0.8322839207348451 2213 12 -0.9713145509117993 2213 13 -1.056932572341914 2213 14 -7.387899496426325 2213 15 -3.705534249645087 2213 16 -2.166061661731 2213 17 -1.470064353345478 2213 18 -5.717358084490069 2213 19 -3.1411083588966164 2213 20 -5.519208693903721 2213 21 -2.6149565721941883 2213 22 -1.938046538589873 2213 23 -5.14592951277719 2213 24 -2.6708119822234266 2213 25 -2.0815832851294864 2214 1 0.050222929162060836 2214 2 -0.31203613906499683 2214 3 -2.0596689769837866 2214 4 -2.317151483192891 2214 5 -0.7620544913538794 2214 6 -1.0885433892946317 2214 7 -0.9784657792072551 2214 8 -0.9853555885625553 2214 9 -5.996266691487293 2214 10 -2.0816592376538523 2214 11 -1.6782732396046995 2214 12 -1.8667034395572912 2214 13 -2.19766938631588 2214 14 -3.5258306517671087 2214 15 -2.948894030239426 2214 16 -2.183648287098464 2214 17 -2.603308604242514 2214 18 -2.1048097280610207 2214 19 -5.106565987054843 2214 20 -3.021950826537246 2214 21 -5.066221615808072 2214 22 -6.736743598431367 2214 23 -7.113003768099137 2214 24 -6.732958810523602 2214 25 -4.296377395174041 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24502.0 3 11.0 4 427.0 5 2941.0 6 3577.0 7 2980.0 8 2170.0 9 1849.0 10 1647.0 11 1477.0 12 1474.0 13 1355.0 14 1410.0 15 1392.0 16 1563.0 17 1525.0 18 1735.0 19 2056.0 20 2644.0 21 3526.0 22 4494.0 23 5497.0 24 6793.0 25 7883.0 26 9033.0 27 10053.0 28 11708.0 29 14143.0 30 17255.0 31 22073.0 32 28441.0 33 36747.0 34 48639.0 35 63860.0 36 86398.0 37 127825.0 38 204994.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.35528211747154 19.32711413376461 9.283337622586641 30.034266126177204 2 13.649213476984064 21.188134842963077 39.37485555057917 25.78779612947369 3 18.453905061597016 24.25101029554817 28.823356596789253 28.471728046065557 4 11.86098315105554 17.41157699017058 37.35127907826977 33.37616078050411 5 12.87438831412322 38.6069751774346 35.30909394763916 13.209542560803012 6 31.995053013718195 38.38565269169244 17.246981172367622 12.372313122221742 7 28.550125380490876 31.599677163222246 21.19067645292901 18.659521003357867 8 25.153534732532357 38.53522305866041 19.308684931686038 17.0025572771212 9 25.86823746714963 15.870343553739874 19.520675622113124 38.74074335699738 10 14.0118150070172 30.55112764776918 34.095760305493 21.341297039720615 11 34.698504859793076 23.10496787550158 23.507927349813727 18.68859991489162 12 22.636817543974157 25.55807199034037 31.55474333452991 20.250367131155567 13 28.74897043063737 22.213636132239127 25.506300116042745 23.53109332108075 14 20.238709500439565 22.568589466424047 27.717159155777736 29.47554187735865 15 22.581697853371363 31.452645594157307 23.04274329872012 22.922913253751215 16 21.782263517482463 29.359911863220912 27.283241219608346 21.574583399688287 17 21.192944257124992 29.353937167703776 27.75199687757401 21.70112169759722 18 21.253438006237037 28.622487008350422 29.14711338012633 20.976961605286206 19 22.793554240064744 27.71624558632528 27.595632338448084 21.894567835161894 20 22.727415127243866 28.129927632334667 28.25315107402805 20.889506166393417 21 22.34978077188659 27.396791790073582 27.359590234656967 22.893837203382862 22 22.132184305642756 28.65838137990359 27.500172954599744 21.709261359853908 23 21.858067581520356 28.12581174446578 27.801179224040816 22.214941449973043 24 22.241207368267162 27.68099559324158 28.493295879367597 21.58450115912366 25 22.14915343618367 27.93954290383594 27.905474110980727 22.00582954899967 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 201.0 3 217.0 4 217.0 5 217.0 6 356.0 7 495.0 8 495.0 9 495.0 10 892.0 11 1289.0 12 1289.0 13 1289.0 14 2177.5 15 3066.0 16 3066.0 17 3066.0 18 5594.0 19 8122.0 20 8122.0 21 8122.0 22 13040.5 23 17959.0 24 17959.0 25 17959.0 26 25847.5 27 33736.0 28 33736.0 29 33736.0 30 44491.0 31 55246.0 32 55246.0 33 55246.0 34 64739.0 35 74232.0 36 74232.0 37 74232.0 38 81791.5 39 89351.0 40 89351.0 41 89351.0 42 94696.0 43 100041.0 44 100041.0 45 100041.0 46 102281.5 47 104522.0 48 104522.0 49 104522.0 50 100257.5 51 95993.0 52 95993.0 53 95993.0 54 84505.0 55 73017.0 56 73017.0 57 73017.0 58 61809.5 59 50602.0 60 50602.0 61 50602.0 62 40592.0 63 30582.0 64 30582.0 65 30582.0 66 23301.0 67 16020.0 68 16020.0 69 16020.0 70 11600.5 71 7181.0 72 7181.0 73 7181.0 74 4989.0 75 2797.0 76 2797.0 77 2797.0 78 1922.0 79 1047.0 80 1047.0 81 1047.0 82 666.0 83 285.0 84 285.0 85 285.0 86 186.0 87 87.0 88 87.0 89 87.0 90 54.5 91 22.0 92 22.0 93 22.0 94 12.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08066863595602124 2 0.03367719753503799 3 0.03028337142685587 4 0.030413903200247488 5 0.02362625098388324 6 0.3660110925901028 7 0.21198359998799107 8 0.21041721870729163 9 0.21485529900260675 10 0.015924876353777657 11 0.001957976600874302 12 0.0028716990146156426 13 0.0 14 0.07361992019287375 15 0.0015663812806994415 16 0.001957976600874302 17 0.0033938261081821234 18 0.0036548896549653633 19 0.0015663812806994415 20 0.0011747859605245811 21 0.0 22 0.0 23 0.0 24 1.3053177339162014E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 766097.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.16271694777184 #Duplication Level Percentage of deduplicated Percentage of total 1 93.32733229244538 81.34663848097064 2 5.204752904555914 9.073208084058008 3 0.7611209452234382 1.9902410853459334 4 0.25739817167409657 0.8974209592201305 5 0.12285623723957197 0.5354241715890558 6 0.07449750963292805 0.3896043207269288 7 0.05041771921300792 0.30761817722409535 8 0.03549212337306206 0.24748719227532845 9 0.0261724864373477 0.20531385244421416 >10 0.12075460864940729 1.9140319068970073 >50 0.009003061453565855 0.5264097389051595 >100 0.008251569235043752 1.2377280476034374 >500 0.0018003423390482237 1.1505051466391862 >1k 1.5002852825401867E-4 0.17836883610091497 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1364 0.17804533890616983 No Hit GTCCTACAGTGGACATTTCTAAATT 969 0.1264852884164799 No Hit CTGTAGGACGTGGAATATGGCAAGA 957 0.12491890713578047 No Hit GTCCTACAGTGTGCATTTCTCATTT 842 0.10990775319574414 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.3053177339162012E-4 0.0 0.0 0.0 6 0.0 1.3053177339162012E-4 0.0 0.0 0.0 7 0.0 1.3053177339162012E-4 0.0 0.0 0.0 8 0.0 1.3053177339162012E-4 0.0 0.0 0.0 9 0.0 1.3053177339162012E-4 0.0 0.0 0.0 10 0.0 1.3053177339162012E-4 0.0 0.0 0.0 11 0.0 1.3053177339162012E-4 0.0 0.0 0.0 12 0.0 1.3053177339162012E-4 0.0 0.0 0.0 13 0.0 1.3053177339162012E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGTCC 40 0.005277056 14.249509 8 TAGGACC 355 0.0 13.443202 4 TCCAACG 85 4.0780324E-6 12.255173 18 GTCCTAC 590 0.0 11.962628 1 TGGCGAG 555 0.0 11.944027 18 TGTAGGA 730 0.0 11.638954 2 TTAGGAC 345 0.0 11.621898 3 CTGTAGG 725 0.0 11.576881 1 GTAGGAC 710 0.0 11.5634365 3 GTAGAAC 225 0.0 11.455871 3 CTAACAC 75 1.9792028E-4 11.45587 3 CTAGCAC 75 1.9792028E-4 11.45587 3 GGACCTG 510 0.0 11.415427 6 GTACTGG 145 1.1350494E-9 11.182215 1 ATGGCGA 610 0.0 11.179054 17 AGGACCT 505 0.0 11.151202 5 TAGAAGC 60 0.0056694737 11.135453 4 TAGGGCC 60 0.0056694737 11.135453 4 GGCGAGG 230 0.0 11.115396 19 TATGGCG 605 0.0 11.114896 16 >>END_MODULE