FastQCFastQC Report
Thu 26 May 2016
SRR1926616_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926616_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823844
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC250.00403246720.3678427
AGCACCG552.2601853E-718.9247135
GTCCTAC4400.015.7125021
ACAGACG400.004419909314.6345698
GTCCTAA1651.8189894E-1214.5038491
TCCTACA5450.014.2397062
GGATAAG751.5688622E-414.18154051
CCCTTAC700.001500918513.2951941
GTACAAT600.00834438913.2951931
GTTATAG600.00834438913.2951931
CCTATAC755.5945144E-513.1148253
AGAAGTG758.836187E-512.490315
TAGGACC1900.012.3967514
TAGGCTG852.0875306E-512.2454015
CCTACAG5750.011.9744063
GTGTAAC1001.3177977E-411.9656731
CTTAAGG650.003748277711.7697153
CTTAGAC851.8007477E-411.5719043
GTTATAC851.8007477E-411.5719043
TGTAGGA3550.011.5057692
Produced by FastQC (version 0.11.5)