FastQCFastQC Report
Thu 26 May 2016
SRR1926616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823844
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17070.20719942124965404No Hit
CTGTAGGACGTGGAATATGGCAAGA11020.13376318817640231No Hit
GTCCTACAGTGGACATTTCTAAATT10660.12939342885303529No Hit
GTACATGGGAAGCAGTGGTATCAAC10330.12538781613994884No Hit
GGTATCAACGCAGAGTACTTTTTTT9920.12041114579944748No Hit
CCCATGTACTCTGCGTTGATACCAC8920.10827292545676123No Hit
GTCCTACAGTGTGCATTTCTCATTT8590.10426731274367479No Hit
TATCAACGCAGAGTACTTTTTTTTT8490.10305349070940616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2950.013.8960154
AAGGCGT551.9020578E-413.865796
AGGCGTG751.4724954E-512.6700757
AATCCCG905.570673E-712.633162519
GTAGGAC6600.012.2792523
GTACTAG550.002993805812.1296021
GGTCTAG550.002993805812.1296021
GGCGAGG2450.011.98861519
TCCAACG951.0707354E-611.968986518
TAGAACC802.7617485E-511.9166414
TGTAGGA7200.011.7863932
GTTATGA657.80428E-411.7297271
AGGACCT4800.011.718035
AAGTGAC657.998281E-411.6954557
CTGTAGG6850.011.6869171
TAGGACG5500.011.6133084
ATTATAC752.0029851E-411.4413733
GTCCTAG752.0109562E-411.4364831
TGGCGAG6000.011.37053718
GGACGTG5200.011.365956
Produced by FastQC (version 0.11.5)