FastQCFastQC Report
Thu 26 May 2016
SRR1926615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707095
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG500.002424402416.0136091
GTCCTAC4000.015.3463761
TGTAGGA4500.015.1883232
GTCCTAA1700.014.9146371
CTGTAGG4600.014.5050821
GTCTTAG658.3348213E-414.3711881
GTGTAAG751.5192485E-414.2343211
TCCTACA4750.014.1011592
AATGGCG350.005668965614.09597616
GTTACAC951.843182E-713.8327723
TAGGACA2100.013.7815034
TCTACAC652.4158193E-413.47808553
GTATAGT904.745879E-513.3446751
GACCTAC600.00813932313.3446751
GTAGGAC4600.013.3315843
TCTTGGA956.288859E-512.9500452
CCTACAG5100.012.8834643
TAGGACC2000.012.8627364
ATAGGAC952.7791666E-612.6800413
GGACGTG3400.012.5568756
Produced by FastQC (version 0.11.5)