FastQCFastQC Report
Thu 26 May 2016
SRR1926615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707095
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15440.21835821212142642No Hit
GTCCTACAGTGGACATTTCTAAATT10930.15457611777766778No Hit
CTGTAGGACGTGGAATATGGCAAGA9860.13944378053868292No Hit
GGTATCAACGCAGAGTACTTTTTTT9100.12869557838762824No Hit
GTCCTACAGTGTGCATTTCTCATTT8860.1253014092872952No Hit
GTCCTAAAGTGTGTATTTCTCATTT8800.12445286701221195No Hit
CTTTAGGACGTGAAATATGGCGAGG8670.12261435874953153No Hit
TATCAACGCAGAGTACTTTTTTTTT8020.11342181743612952No Hit
GTACATGGGAAGCAGTGGTATCAAC7650.10818914007311607No Hit
CCCATGTACTCTGCGTTGATACCAC7380.10437069983524137No Hit
CTGTAGGACCTGGAATATGGCGAGA7210.10196649672250546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAC400.00515256114.3006511
CTAACCT603.9689252E-412.7107854
TCGAACT604.1810863E-412.63257919
GCCTCGA550.003128093212.05837116
TAGGACC2550.011.9630924
TCTAACC657.7555864E-411.7380443
CCCTTAC657.778787E-411.7338681
GGCGAGG2100.011.73025319
CACGTTT658.1971363E-411.66084218
GTCCTAC6000.011.5994171
TTAGGAC3800.011.2940063
CTAAATT2800.011.16629819
TTGGACA600.00572024311.1219374
GACGTGA3200.010.9882317
AGGACCT4700.010.952915
TCCTACA6900.010.921692
GTCCTAA3150.010.8957341
AACAGAT700.001485297510.8609537
TGGCGAG6300.010.82792518
CTCGAAC700.001523523610.82792518
Produced by FastQC (version 0.11.5)