FastQCFastQC Report
Thu 26 May 2016
SRR1926614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853227
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG405.473826E-419.9926871
TGTAGGA4000.016.7239552
TAGGACC1500.016.4526024
GGTCTAT500.002445157415.9941481
AGGACGG400.002861623415.6223935
GTCTAAG604.585881E-415.5498661
GTCCTAC4050.015.4675921
GTAGGAC4200.014.8530093
GTTTTAG902.9307157E-614.8093971
CTGTAGG4150.014.773711
GTATTGA550.004612006214.5401351
CCCTAGG550.004612006214.5401351
GTCTATG1303.426976E-914.3537231
AGGACGT2750.014.0128135
GGACGTG2700.013.8810046
TAGGACG3100.013.8451094
GTCATAG1101.5878395E-613.3284571
GTATAGT700.001473052413.3284571
GTATAGA700.001473052413.3284571
TCCTACA4650.013.2118152
Produced by FastQC (version 0.11.5)