FastQCFastQC Report
Thu 26 May 2016
SRR1926614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853227
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13750.16115289366135857No Hit
CTGTAGGACGTGGAATATGGCAAGA10870.12739868757083403No Hit
GTCCTACAGTGGACATTTCTAAATT10410.12200739076470857No Hit
GTCCTACAGTGTGCATTTCTCATTT9210.10794313822699No Hit
GGTATCAACGCAGAGTACTTTTTTT8940.10477868140600334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCA250.006029455519.0002359
CATTCGT350.002208401216.24201419
GATCTAT508.434488E-515.2566661
ATCCCGT400.005344324314.22259710
TCCAACG1051.4224497E-913.5350118
TAGGACC2850.013.3838314
CGTGCAC500.001520472913.27442410
GTTTAAG655.259892E-513.2028861
GTCCTAA2450.012.8436231
GTAGGAC6750.012.716143
TAATACC603.9602863E-412.714644
TGTAGGA7300.012.6778262
AGGACCT4400.012.5701555
GTCCTAG1551.8189894E-1212.3037641
GTATTAC959.922933E-712.0447371
GGACGTG5800.011.6719776
CGGTTTC658.147393E-411.66982313
AAAGTGT2800.011.5780386
CTGTAGG7450.011.5192971
AGGACGT6000.011.4431755
Produced by FastQC (version 0.11.5)