FastQCFastQC Report
Thu 26 May 2016
SRR1926613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706604
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG400.0020930916.3709563
CTAACTA400.002400016416.0390134
GTCCTAG758.918067E-615.9297631
GGTTTAC604.7262284E-415.487271
ACCAGTC400.003331154815.2695417
TAGGACC1155.638867E-1114.8767654
GTCCTAA1750.014.4126421
GTATACA658.6728804E-414.2959421
GTATATC1105.4387783E-913.8905073
TATAGAA700.001272689613.5883552
GTATAGA600.0084268113.2748031
TGTAGGA3400.013.1886972
ACTTATT450.00964761113.0067718
GGACCTG2100.012.867656
CTATGAC600.002077867312.73296453
TCCTAGA750.0021395612.6824642
GTATAAA958.275229E-512.5761281
TATACAG1308.563802E-911.9955185
GTTCTAT907.409417E-411.7998251
GTAGGAC3350.011.7284463
Produced by FastQC (version 0.11.5)