##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926613_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 706604 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.17108309604814 27.0 7.0 31.0 2.0 31.0 2 16.494776423569636 18.0 2.0 30.0 2.0 31.0 3 19.966213041533873 24.0 15.0 31.0 2.0 31.0 4 22.141090341973722 28.0 2.0 35.0 2.0 37.0 5 22.899261821331326 30.0 2.0 35.0 2.0 37.0 6 23.164040962123057 30.0 2.0 35.0 2.0 37.0 7 22.935961302228687 30.0 7.0 35.0 2.0 37.0 8 23.34385313414586 30.0 8.0 35.0 2.0 37.0 9 26.11644853411529 31.0 17.0 37.0 2.0 39.0 10 26.810891248846595 32.0 23.0 37.0 2.0 39.0 11 23.936629285993288 30.0 7.0 37.0 2.0 39.0 12 25.040054967138595 31.0 10.0 37.0 2.0 39.0 13 21.720130370051685 27.0 2.0 35.0 2.0 39.0 14 25.0643231569592 31.0 17.0 38.0 2.0 40.0 15 26.136411625181857 31.0 17.0 38.0 2.0 40.0 16 26.52212837742215 31.0 17.0 38.0 2.0 40.0 17 25.796129090692947 31.0 10.0 38.0 2.0 40.0 18 25.86197926985978 31.0 11.0 38.0 2.0 40.0 19 26.26127364124743 31.0 16.0 38.0 2.0 40.0 20 26.506892120622016 31.0 16.0 39.0 2.0 40.0 21 24.35535745622725 30.0 2.0 37.0 2.0 40.0 22 25.281565063316936 31.0 2.0 38.0 2.0 40.0 23 25.258901732795174 31.0 2.0 38.0 2.0 40.0 24 24.35993993806998 31.0 2.0 38.0 2.0 40.0 25 24.156043554805805 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.455122022663666 1101 2 10.283893342310844 1101 3 6.670063102762086 1101 4 8.250454780254127 1101 5 8.063034712327408 1101 6 7.290260018254152 1101 7 6.972950091122257 1101 8 5.506019760994992 1101 9 5.406063723324767 1101 10 5.1075592727063 1101 11 7.8657222081909595 1101 12 5.342550286236705 1101 13 10.106671324687227 1101 14 7.49411758032414 1101 15 6.52215418598502 1101 16 6.062974636868589 1101 17 1.7313267953949278 1101 18 4.429780438991834 1101 19 4.988254909275369 1101 20 2.924021035421024 1101 21 0.17595888879213106 1101 22 4.173537426806497 1101 23 5.378110313489113 1101 24 6.914822398957941 1101 25 7.366686088711841 1102 1 5.957706711862528 1102 2 10.916361888165216 1102 3 7.266332457299157 1102 4 8.583567563760614 1102 5 8.604135655790557 1102 6 6.798191428547767 1102 7 6.740284856094817 1102 8 6.946881453037786 1102 9 6.173583569584771 1102 10 5.480785684515389 1102 11 8.292689906024798 1102 12 5.769126540372014 1102 13 10.211540187823807 1102 14 7.851476012485719 1102 15 5.944924343400849 1102 16 6.385976951996888 1102 17 7.104440997642985 1102 18 7.098691296879068 1102 19 5.77619496099495 1102 20 6.293854075332803 1102 21 4.827196095934852 1102 22 6.231491730430093 1102 23 6.5696455853393445 1102 24 7.768274777607171 1102 25 7.767609560501455 1103 1 5.392263365397284 1103 2 9.551699282626696 1103 3 6.938955312403767 1103 4 8.512202901155074 1103 5 7.993539658333244 1103 6 7.359045580642796 1103 7 7.275048551442456 1103 8 6.955741009342599 1103 9 6.082532996782373 1103 10 5.313051925540751 1103 11 6.409743256654792 1103 12 4.946427265117993 1103 13 9.225522982828501 1103 14 7.590818540149421 1103 15 6.686321347571575 1103 16 6.422497214794525 1103 17 4.124613127304169 1103 18 2.855577579312797 1103 19 5.152277745836859 1103 20 5.5934509515720165 1103 21 7.628214765566661 1103 22 3.185954487812559 1103 23 5.636365257898433 1103 24 7.124041958191871 1103 25 2.7554759936233637 1104 1 5.268827829358148 1104 2 10.074570005735882 1104 3 6.65462564001465 1104 4 7.146266807734758 1104 5 7.141890940956202 1104 6 6.517721824572188 1104 7 6.569791073648844 1104 8 6.700627158177912 1104 9 5.746955275227393 1104 10 4.774030396086793 1104 11 7.69196683597902 1104 12 4.780467907091868 1104 13 8.947807567119074 1104 14 6.536251407221275 1104 15 5.934090691929747 1104 16 5.606495043431316 1104 17 6.07599850226822 1104 18 3.3733384902513635 1104 19 4.138090213726855 1104 20 3.4841396162159555 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1.220364865769767 2204 6 3.949192735799077 2204 7 5.193664809474772 2204 8 5.265854709511043 2204 9 3.938329521830724 2204 10 3.601911180640883 2204 11 5.092357580692472 2204 12 5.214340110585585 2204 13 3.1610928873765083 2204 14 4.471965601725312 2204 15 4.850229342455847 2204 16 4.766639082659019 2204 17 -0.6209496027476114 2204 18 3.0169939964317685 2204 19 4.080560077860067 2204 20 4.329035673014403 2204 21 5.921127221115977 2204 22 5.491642615580979 2204 23 4.312111171554932 2204 24 5.7079679673003625 2204 25 5.999250948392845 2205 1 6.315751943923509 2205 2 10.440586255696669 2205 3 7.344078850793583 2205 4 8.096124071592712 2205 5 8.130751247760479 2205 6 7.815456868647853 2205 7 7.8414540281301335 2205 8 7.4540512570579835 2205 9 6.408425928049176 2205 10 5.91464588687953 2205 11 7.9806828381122195 2205 12 7.467747136630404 2205 13 10.527931167206912 2205 14 7.922464037017058 2205 15 7.00640615448896 2205 16 6.547226605372529 2205 17 7.2612480552374485 2205 18 7.0699379193067955 2205 19 5.577231287228127 2205 20 5.859454106287167 2205 21 -0.4350647332551105 2205 22 4.217631914995476 2205 23 5.765511888292263 2205 24 7.377814524942195 2205 25 7.9076309706016055 2206 1 3.9175612150785035 2206 2 8.419075024800069 2206 3 5.959105953602968 2206 4 1.573757153336235 2206 5 5.391106817282058 2206 6 6.004748086370736 2206 7 5.825844985400149 2206 8 6.148451822681832 2206 9 4.8432075646116814 2206 10 3.902703730378178 2206 11 3.0612238065617667 2206 12 4.362895220096181 2206 13 7.5752997472855945 2206 14 -0.27835478810486336 2206 15 2.111442986511971 2206 16 3.0566355405372896 2206 17 4.126538934552897 2206 18 -1.2822408806762837 2206 19 0.7938609909403347 2206 20 2.401620880720486 2206 21 3.233936541815744 2206 22 -2.2197802350398774 2206 23 -3.090001433478818 2206 24 0.9737107305488806 2206 25 2.6570457070366054 2207 1 1.461122302515598 2207 2 -13.802720830917504 2207 3 -6.177000360023966 2207 4 -4.608198368716145 2207 5 1.1909219574395387 2207 6 1.6208936284712934 2207 7 2.8432857862362972 2207 8 2.8620473900130925 2207 9 0.6091980649730928 2207 10 0.4004230484176965 2207 11 -1.327224251046065 2207 12 -2.7756095600991415 2207 13 0.3630004305603016 2207 14 -5.118478300764227 2207 15 -1.3800337473084525 2207 16 -0.45782344996183255 2207 17 0.6821084727256483 2207 18 0.5185952660560602 2207 19 -0.037949408076723756 2207 20 -0.16745111295138315 2207 21 -4.046649331222369 2207 22 -4.624896674423578 2207 23 -1.7246118825853287 2207 24 -0.03138624337856655 2207 25 0.4246281593617347 2208 1 4.636952245274728 2208 2 6.109316206119534 2208 3 6.0141332884333245 2208 4 7.754706505732887 2208 5 3.302896917051818 2208 6 5.912838272361277 2208 7 6.544507639484269 2208 8 6.406583579406579 2208 9 4.898384127718661 2208 10 4.533178986908691 2208 11 6.326094522762748 2208 12 5.768411810490619 2208 13 8.53021681900044 2208 14 2.3235503595600697 2208 15 4.319454176264017 2208 16 4.775008028527967 2208 17 5.584534727736717 2208 18 0.47369508854952613 2208 19 3.1262628318961525 2208 20 3.9416306397760366 2208 21 6.628777980850071 2208 22 5.69065733542903 2208 23 1.5871289778227364 2208 24 2.7770562863886816 2208 25 4.2037559341478286 2209 1 2.711962733108841 2209 2 4.688379492707092 2209 3 4.973800074960952 2209 4 0.6197805198764819 2209 5 4.989992386307481 2209 6 5.281942611328077 2209 7 5.908937400960955 2209 8 4.442152319667876 2209 9 4.278493201193431 2209 10 3.437080461198512 2209 11 -0.9648679607563686 2209 12 2.8403358785716435 2209 13 2.03581282763043 2209 14 3.425716846435293 2209 15 3.8847933827844514 2209 16 3.566427481961984 2209 17 1.2297163170930645 2209 18 3.347721218239087 2209 19 3.988280391526981 2209 20 4.185847348394038 2209 21 6.31066698959992 2209 22 5.089756879957378 2209 23 0.5035709051869937 2209 24 -2.9760001681240844 2209 25 0.2571315184700147 2210 1 -2.7603011555511117 2210 2 -13.802720830917504 2210 3 -7.973536752858676 2210 4 -3.033107376343871 2210 5 -1.3688984989074342 2210 6 -1.8649329552896887 2210 7 -1.8397686501046984 2210 8 -20.642799136642804 2210 9 -13.47784467607098 2210 10 -7.950802022963661 2210 11 -10.686162074524976 2210 12 -5.562318502448242 2210 13 -6.143889481697542 2210 14 -4.822420526436282 2210 15 -6.521977733857764 2210 16 -6.079873911986404 2210 17 -5.513732894724452 2210 18 -5.6978815531742555 2210 19 -10.801446038547578 2210 20 -8.02202925667956 2210 21 -6.172040522996166 2210 22 -10.041404487287856 2210 23 -8.398620566288436 2210 24 -11.064734570682312 2210 25 -8.87399276106691 2211 1 -0.06078905663324008 2211 2 4.555023276826603 2211 3 0.6030782940449946 2211 4 1.6001750803681283 2211 5 0.8931746948295967 2211 6 0.16080404116037883 2211 7 -20.22941211360215 2211 8 -13.302731796575463 2211 9 -9.171761775760576 2211 10 -7.155373201643499 2211 11 -9.791189427854537 2211 12 -4.719452948207476 2211 13 -6.177485201201581 2211 14 -4.676028765019055 2211 15 -5.127375228898721 2211 16 -11.539175473155534 2211 17 -7.545936652734664 2211 18 -5.781074832887061 2211 19 -8.64274670794672 2211 20 -6.42004163856291 2211 21 -4.432108663369906 2211 22 -5.847291598353234 2211 23 -7.939386173692412 2211 24 -21.759980750648356 2211 25 -21.562447769555874 2212 1 -1.6953753214754457 2212 2 -13.802720830917504 2212 3 -8.039859308673229 2212 4 -19.49915151895847 2212 5 -20.134603082948182 2212 6 -20.407377777021438 2212 7 -9.921368237916594 2212 8 -9.693987436459988 2212 9 -5.612088258134591 2212 10 -6.128629530932734 2212 11 -6.172003971831785 2212 12 -3.5935104027036857 2212 13 -6.854622201921762 2212 14 -4.4749549556271475 2212 15 -4.23698442260298 2212 16 -4.476892066265183 2212 17 -4.5210749953318725 2212 18 -3.6280439143168373 2212 19 -4.088916203019323 2212 20 -4.25635726281272 2212 21 -2.1076237163986704 2212 22 -3.6729322215258513 2212 23 -3.617260283467804 2212 24 -2.655775997449087 2212 25 -3.302849963340151 2213 1 -18.558263804107987 2213 2 -13.802720830917504 2213 3 -8.467229026262325 2213 4 -5.842780862587814 2213 5 -20.134603082948182 2213 6 -10.360080479724141 2213 7 -20.22941211360215 2213 8 -10.4381659320096 2213 9 -8.300910404909203 2213 10 -7.162159208429504 2213 11 -6.53464717131228 2213 12 -5.08336556212009 2213 13 -18.93506095877734 2213 14 -12.126059965050255 2213 15 -6.894551496074989 2213 16 -6.059764243744304 2213 17 -4.683554790352801 2213 18 -4.702671754483983 2213 19 -5.576150891350903 2213 20 -5.540649259170529 2213 21 -3.67508540634665 2213 22 -4.602221853283492 2213 23 -4.391188625494866 2213 24 -3.436621677289285 2213 25 -4.1358068429149455 2214 1 -18.558263804107987 2214 2 -13.802720830917504 2214 3 -12.345214154247454 2214 4 -19.49915151895847 2214 5 -20.134603082948182 2214 6 -20.407377777021438 2214 7 -16.293962378152415 2214 8 -14.948619242462911 2214 9 -14.070751674750474 2214 10 -14.039043235313532 2214 11 -14.451771438436547 2214 12 -12.463753092507618 2214 13 -18.93506095877734 2214 14 -16.753830592820883 2214 15 -23.543200144723638 2214 16 -18.309978743958805 2214 17 -23.239539346337356 2214 18 -17.727274929087155 2214 19 -15.136325351525363 2214 20 -13.872566891088162 2214 21 -11.372897504158749 2214 22 -12.376560477622116 2214 23 -12.337341921648163 2214 24 -11.237229427897034 2214 25 -10.96879697590508 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 128977.0 3 0.0 4 593.0 5 2423.0 6 3240.0 7 3026.0 8 2546.0 9 2498.0 10 2319.0 11 2233.0 12 2166.0 13 2326.0 14 3560.0 15 7267.0 16 10234.0 17 11049.0 18 11580.0 19 13573.0 20 15722.0 21 18229.0 22 18972.0 23 20735.0 24 22287.0 25 22422.0 26 19110.0 27 15362.0 28 16352.0 29 19135.0 30 18267.0 31 21233.0 32 26979.0 33 33616.0 34 40452.0 35 45929.0 36 45015.0 37 45381.0 38 31796.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19333304075174 18.36047027614826 12.282900393847335 26.163296289252663 2 14.29623748401041 20.72361166247629 40.0527105112258 24.927440342287504 3 18.802854142416493 24.39923238370029 28.60404391771944 28.193869556163776 4 11.767871396605646 17.318047395349446 37.3104251449209 33.603656063124006 5 12.9558747774829 37.201435846608284 36.31642094692845 13.526268428980368 6 31.651723127698983 36.72800221132376 17.92049496576255 13.699779695214703 7 27.471938556929093 30.93122706984787 22.13648649701275 19.460347876210285 8 24.822557136908117 36.86341033241065 20.795874874458026 17.518157656223206 9 26.294277157081368 15.598905131205745 19.84038830439846 38.266429407314426 10 14.65525536825566 29.50382682555162 34.08711288309547 21.75380492309725 11 33.27884719109204 22.463922283084226 24.39500904939411 19.862221476429625 12 23.46003683572976 24.646765765887473 31.526070960565956 20.367126437816815 13 28.079232035955844 21.49596220061328 25.0235909753796 25.401214788051274 14 20.698552413004553 22.246384628107492 27.491352591346118 29.563710367541834 15 22.757891975398607 30.793861311102688 23.42714314862856 23.021103564870142 16 22.07081887512333 28.164908849517538 27.3589896425537 22.405282632805427 17 21.63295039811179 27.937321978830504 28.601982909967727 21.827744713089974 18 21.58251669562443 27.304513531108398 30.02395662227434 21.089013150992827 19 22.984886830773295 26.82192544246453 27.071848598275817 23.12133912848636 20 23.164156141289617 27.65599297156977 27.7735119584085 21.406338928732115 21 22.140469316269797 26.292306758870527 26.517538316741867 25.049685608117805 22 22.62204805966027 27.77283869631266 27.64869032822354 21.956422915803525 23 22.118652832856576 27.620865194026127 27.835371425899712 22.42511054721758 24 22.58112174677107 27.35394975233859 27.855804296944864 22.20912420394548 25 22.682384278609785 26.905453463494805 27.917935929950836 22.49422632794457 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 787.0 1 787.0 2 1624.5 3 2462.0 4 2462.0 5 2462.0 6 3489.5 7 4517.0 8 4517.0 9 4517.0 10 5245.5 11 5974.0 12 5974.0 13 5974.0 14 7346.0 15 8718.0 16 8718.0 17 8718.0 18 11936.0 19 15154.0 20 15154.0 21 15154.0 22 20844.5 23 26535.0 24 26535.0 25 26535.0 26 34119.0 27 41703.0 28 41703.0 29 41703.0 30 50530.0 31 59357.0 32 59357.0 33 59357.0 34 65894.0 35 72431.0 36 72431.0 37 72431.0 38 76529.0 39 80627.0 40 80627.0 41 80627.0 42 82810.0 43 84993.0 44 84993.0 45 84993.0 46 84982.0 47 84971.0 48 84971.0 49 84971.0 50 79592.0 51 74213.0 52 74213.0 53 74213.0 54 65631.0 55 57049.0 56 57049.0 57 57049.0 58 48506.0 59 39963.0 60 39963.0 61 39963.0 62 32245.5 63 24528.0 64 24528.0 65 24528.0 66 18890.5 67 13253.0 68 13253.0 69 13253.0 70 9681.5 71 6110.0 72 6110.0 73 6110.0 74 4172.0 75 2234.0 76 2234.0 77 2234.0 78 1477.5 79 721.0 80 721.0 81 721.0 82 475.5 83 230.0 84 230.0 85 230.0 86 142.5 87 55.0 88 55.0 89 55.0 90 36.5 91 18.0 92 18.0 93 18.0 94 9.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.933835642028631 2 35.831102003385205 3 5.531103701649014 4 13.477987670604751 5 13.185320207641055 6 14.497653565504864 7 9.157746064273624 8 7.562368738359816 9 0.1084058397631488 10 1.462629704898359 11 5.5414348064828385 12 5.717912720562012 13 15.864189843250251 14 1.6004721173387075 15 2.896105881087568 16 2.8914356556147434 17 2.8642634346819436 18 1.5232011140610582 19 1.4704134140197338 20 0.2882802814589218 21 4.3666608170913275 22 1.622974112798682 23 0.11279302126792376 24 8.999241442165626 25 0.11519889499634872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 706604.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.15340360920301 #Duplication Level Percentage of deduplicated Percentage of total 1 96.27480675396379 88.7205112419605 2 2.6680539162104147 4.917404987833061 3 0.506928419342464 1.4014553768592422 4 0.1970692417657566 0.7264240550159751 5 0.10403892818818665 0.47937706701974264 6 0.054040466829068164 0.298800777055732 7 0.0465348871981198 0.30018437693139477 8 0.03243445922733532 0.23911566496182968 9 0.0159698125419484 0.13245053226673348 >10 0.08800131973800228 1.3963857902070713 >50 0.007399180928332386 0.4649654804026549 >100 0.004565193598586267 0.8492299433356871 >500 1.5742046795021682E-4 0.07369470615037896 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.4152198402499845E-4 10 0.0 0.0 0.0 1.4152198402499845E-4 1.4152198402499845E-4 11 0.0 0.0 0.0 1.4152198402499845E-4 1.4152198402499845E-4 12 0.0 0.0 0.0 1.4152198402499845E-4 4.2456595207499535E-4 13 0.0 0.0 0.0 1.4152198402499845E-4 4.2456595207499535E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAACG 40 0.00209309 16.370956 3 CTAACTA 40 0.0024000164 16.039013 4 GTCCTAG 75 8.918067E-6 15.929763 1 GGTTTAC 60 4.7262284E-4 15.48727 1 ACCAGTC 40 0.0033311548 15.269541 7 TAGGACC 115 5.638867E-11 14.876765 4 GTCCTAA 175 0.0 14.412642 1 GTATACA 65 8.6728804E-4 14.295942 1 GTATATC 110 5.4387783E-9 13.890507 3 TATAGAA 70 0.0012726896 13.588355 2 GTATAGA 60 0.00842681 13.274803 1 TGTAGGA 340 0.0 13.188697 2 ACTTATT 45 0.009647611 13.006771 8 GGACCTG 210 0.0 12.86765 6 CTATGAC 60 0.0020778673 12.7329645 3 TCCTAGA 75 0.00213956 12.682464 2 GTATAAA 95 8.275229E-5 12.576128 1 TATACAG 130 8.563802E-9 11.995518 5 GTTCTAT 90 7.409417E-4 11.799825 1 GTAGGAC 335 0.0 11.728446 3 >>END_MODULE