FastQCFastQC Report
Thu 26 May 2016
SRR1926613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706604
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16180.2289825701524475No Hit
CCCATGTACTCTGCGTTGATACCAC13070.18496923312067295No Hit
GTACATGGGAAGCAGTGGTATCAAC12570.17789313391942305No Hit
CTGTAGGACGTGGAATATGGCAAGA10580.14973025909844837No Hit
GTCCTACAGTGGACATTTCTAAATT10260.1452015556096484No Hit
GGTATCAACGCAGAGTACTTTTTTT9040.1279358735585986No Hit
CTTTAGGACGTGAAATATGGCGAGG8280.11718020277269872No Hit
GTCCTAAAGTGTGTATTTCTCATTT7700.10897192769924881No Hit
TATCAACGCAGAGTACTTTTTTTTT7440.10529235611459885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA350.00220484416.24537719
TAGGACC3100.011.9933034
GATATAC1800.011.645811
AATCCCG752.119007E-411.37176419
CCTTTAA1104.765352E-711.2608251
CACTATG855.309227E-511.1772637
CTCTAAG600.00574127611.1164561
CCATTAT600.00574127611.1164561
TGTAGGA7400.011.0829982
TGGCGAG5450.010.95445218
TATAACT700.001446985410.8950164
GTGTAAC803.662729E-410.71943951
GACGTGA2950.010.6278727
TAAGGCT909.1676695E-510.5923774
GTAGGAC7300.010.5801263
TAGGACT1451.3604222E-810.5193254
GTGCTAA750.002579822210.1636161
GGACCTG4600.010.15341956
CTGTAGG7700.010.1471181
ATAATAC856.363412E-410.0960673
Produced by FastQC (version 0.11.5)