##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926613_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 706604 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.44871667864886 31.0 28.0 31.0 23.0 34.0 2 28.826512445443274 31.0 28.0 33.0 23.0 34.0 3 29.229242970603053 31.0 29.0 33.0 25.0 34.0 4 33.0173024777669 35.0 33.0 37.0 27.0 37.0 5 32.72506948729416 35.0 33.0 37.0 26.0 37.0 6 32.85795013897459 35.0 33.0 37.0 26.0 37.0 7 32.69699011044376 35.0 33.0 37.0 25.0 37.0 8 32.66968627406581 35.0 33.0 37.0 25.0 37.0 9 33.913305047806126 38.0 34.0 39.0 25.0 39.0 10 33.80226831435995 37.0 33.0 39.0 25.0 39.0 11 34.064614692246295 37.0 34.0 39.0 26.0 39.0 12 33.85721280943782 37.0 34.0 39.0 25.0 39.0 13 34.00317292288184 37.0 34.0 39.0 26.0 39.0 14 34.652988095170706 38.0 34.0 40.0 24.0 41.0 15 34.78141646523371 39.0 34.0 40.0 25.0 41.0 16 34.748733378242974 39.0 34.0 40.0 25.0 41.0 17 34.67497778104851 38.0 34.0 40.0 24.0 41.0 18 34.633639209514804 38.0 34.0 40.0 24.0 41.0 19 34.68114530911232 39.0 34.0 40.0 24.0 41.0 20 34.670618903940536 39.0 34.0 40.0 24.0 41.0 21 34.59647978216936 39.0 34.0 40.0 23.0 41.0 22 34.529405437840715 39.0 34.0 40.0 22.0 41.0 23 34.19820861472621 39.0 34.0 40.0 18.0 41.0 24 34.28071734663263 39.0 34.0 40.0 18.0 41.0 25 34.07788662390816 39.0 34.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.46734967143570927 1101 2 0.48638955483241375 1101 3 0.4949184323941509 1101 4 0.2855447442614718 1101 5 0.15769400213069673 1101 6 -0.13556533696455375 1101 7 0.1362249074498436 1101 8 0.07192589052202436 1101 9 0.5226503384624408 1101 10 0.3680240742807541 1101 11 0.05745157310431637 1101 12 0.31160605949251163 1101 13 0.17520001668969343 1101 14 0.4171731547931685 1101 15 0.19234736255862828 1101 16 0.22799783567631948 1101 17 0.3363314076402304 1101 18 0.39386891637570187 1101 19 0.41203614008647804 1101 20 0.23982890783084088 1101 21 0.3751495301943777 1101 22 0.4458360439847624 1101 23 0.38842141039019396 1101 24 0.4458362539110823 1101 25 0.525373150731717 1102 1 0.6002070814584748 1102 2 0.589777767669446 1102 3 0.42176489231147585 1102 4 0.36706756987878464 1102 5 0.5009004870694653 1102 6 0.27329857347415754 1102 7 0.6029663804905283 1102 8 0.6172153460477006 1102 9 0.8382913194577526 1102 10 0.9811500133909448 1102 11 0.655298310321129 1102 12 0.797381240543281 1102 13 0.6556213829378308 1102 14 0.9553173118349676 1102 15 0.7429242099858655 1102 16 0.6475951710216847 1102 17 0.7544222973846644 1102 18 0.8541080388195468 1102 19 0.9630340958088439 1102 20 1.0852647516918807 1102 21 1.0460827397102648 1102 22 1.1196660658462818 1102 23 1.2358912137655125 1102 24 1.1221581018864697 1102 25 1.226686776454791 1103 1 0.2971508732927788 1103 2 0.12771185304878685 1103 3 0.0972512612285037 1103 4 -0.08147187866066474 1103 5 -0.07608060815979911 1103 6 -0.22977317777277761 1103 7 -0.060557793982553676 1103 8 -0.13770812420423795 1103 9 0.23585543964960465 1103 10 0.08638464417301606 1103 11 -0.23513293047507489 1103 12 -0.07130164273814188 1103 13 -0.21147929438182445 1103 14 0.17491912997692793 1103 15 0.0020931333883638104 1103 16 0.0752949344878715 1103 17 0.06816337754909796 1103 18 0.0018968362433824382 1103 19 0.15885300695622107 1103 20 0.14628330176152815 1103 21 0.15201282374253822 1103 22 0.30595188716859667 1103 23 0.3401095661590503 1103 24 0.3689902027331726 1103 25 0.3871710829245032 1104 1 0.40268235644370876 1104 2 0.5918543099850311 1104 3 0.4590649957470383 1104 4 0.32206951133490946 1104 5 0.2818964051171804 1104 6 -0.05470766528905102 1104 7 0.35606993678133847 1104 8 0.41961231019764966 1104 9 0.6868094500475976 1104 10 0.6461995613090181 1104 11 0.2725188629998456 1104 12 0.5272003751432948 1104 13 0.22357658914004475 1104 14 0.8034014698792831 1104 15 0.34099058494467016 1104 16 0.24733152454290064 1104 17 0.102805668078787 1104 18 0.5134441587134333 1104 19 0.5036131391767285 1104 20 0.5267003198206766 1104 21 0.5129353946107287 1104 22 0.30382769941030574 1104 23 0.7403428584562022 1104 24 0.395179040246056 1104 25 0.6171132768414509 1105 1 0.358915025766418 1105 2 0.22063837373005057 1105 3 0.39998929853588194 1105 4 -0.0015607675495488138 1105 5 0.2762262855952642 1105 6 -0.10919053381494592 1105 7 -0.1246758799517309 1105 8 -0.037837889005537306 1105 9 0.4744046691711006 1105 10 0.47720832624925436 1105 11 0.19362165184447377 1105 12 0.2964816500436882 1105 13 0.05382678834323684 1105 14 0.35527079258844196 1105 15 0.19399775625941373 1105 16 0.3149458671723835 1105 17 0.16558495094731285 1105 18 0.1328736407851494 1105 19 0.4255605495352981 1105 20 0.4566541046812347 1105 21 0.36173220735972933 1105 22 0.5530675400477563 1105 23 0.6491269222012193 1105 24 0.4582897972181641 1105 25 0.5625825995506162 1106 1 1.00129535883541 1106 2 1.1101920612796157 1106 3 0.8232311700664141 1106 4 0.8409001364948594 1106 5 0.7332089977284966 1106 6 -0.22415706831137783 1106 7 -0.06508888550288106 1106 8 0.03640477554640853 1106 9 0.18245351929004983 1106 10 0.17188302886622608 1106 11 0.11894694922749949 1106 12 0.27190940579309597 1106 13 0.16253352902523233 1106 14 0.45113819940843314 1106 15 0.40894398940771026 1106 16 0.17562532791782104 1106 17 0.09236242913922865 1106 18 0.2142021102538223 1106 19 0.19709361852815732 1106 20 0.20986393814673932 1106 21 0.21555615660636107 1106 22 0.18387547026186724 1106 23 0.42886300467544913 1106 24 0.3543652928565493 1106 25 0.10925920541897938 1107 1 0.9784918597432544 1107 2 1.0128267907184672 1107 3 0.9183367888833729 1107 4 0.8311836339948542 1107 5 0.8932486794176597 1107 6 0.45095155112714025 1107 7 0.8475434250675704 1107 8 0.818455547287904 1107 9 0.9324062135726479 1107 10 0.9959388281797601 1107 11 0.6601421151649305 1107 12 0.6617079048699424 1107 13 0.6409495682660165 1107 14 1.1179240744417243 1107 15 0.7447962118578673 1107 16 0.9151744386009497 1107 17 0.8373285802909436 1107 18 1.1672705519820639 1107 19 1.1750617078364556 1107 20 0.6953969618240947 1107 21 0.9492534428809662 1107 22 1.0031339493141616 1107 23 1.2725122503865478 1107 24 0.9889183686467362 1107 25 1.0443771941452127 1108 1 1.2378057605906996 1108 2 1.0999917283626388 1108 3 1.0660084678649575 1108 4 0.9282015327699398 1108 5 -0.3691040658424356 1108 6 0.496680392702622 1108 7 0.9032269954476249 1108 8 1.0011716956590035 1108 9 1.2942347011199828 1108 10 1.4617399556422228 1108 11 1.0080037604706718 1108 12 1.0950605637817077 1108 13 1.0234868522409997 1108 14 1.4728120066459525 1108 15 1.1326482354926384 1108 16 1.1544487425717378 1108 17 1.2402533215543485 1108 18 1.1317924906254078 1108 19 1.1164410607174062 1108 20 1.1914489928601455 1108 21 1.2868951466568532 1108 22 1.1681486902074951 1108 23 1.386020212936046 1108 24 1.0443268579210425 1108 25 1.1799742928413508 1109 1 0.8725852012050161 1109 2 0.013382233379175545 1109 3 0.8227261406411444 1109 4 0.9375339692925522 1109 5 -0.24618774949244937 1109 6 0.5607463082903124 1109 7 0.6460328919780949 1109 8 0.9602322864330617 1109 9 1.4143204586447808 1109 10 -1.1384478141016245 1109 11 0.3722602483356994 1109 12 0.9437237553068485 1109 13 1.0864653848344616 1109 14 1.5623865820621319 1109 15 1.2383205632769574 1109 16 1.4463774461197474 1109 17 1.4755288105964368 1109 18 1.3718631144693632 1109 19 1.419392128482663 1109 20 1.5856225257338679 1109 21 1.6014515056053469 1109 22 1.5631657856617878 1109 23 1.5969585011485918 1109 24 1.5113494463409722 1109 25 1.696273827620793 1110 1 0.6478136368775296 1110 2 -0.6453074596032806 1110 3 0.17173929853588277 1110 4 0.6147086768948924 1110 5 0.8167873967063812 1110 6 0.7085441884072736 1110 7 0.7470380089371531 1110 8 0.9472662776611287 1110 9 1.1185602247266573 1110 10 0.9702236040270336 1110 11 0.6730369296222491 1110 12 0.7685135944881338 1110 13 0.6868948438987914 1110 14 0.9760013481440026 1110 15 0.7076005340371907 1110 16 0.38107225606126605 1110 17 0.8758668953917592 1110 18 0.812844474118485 1110 19 0.6372466606464116 1110 20 0.9981499380145706 1110 21 0.7915099851375089 1110 22 0.886402262269975 1110 23 1.2550908110901062 1110 24 1.002385630551501 1110 25 1.2155812106617319 1111 1 -0.20646077438970778 1111 2 0.10684539949117422 1111 3 0.16678711274353475 1111 4 0.06320675782125562 1111 5 0.4180835845804367 1111 6 -0.40073526449410224 1111 7 0.0716419967071289 1111 8 0.18309887914434597 1111 9 0.5049445598814799 1111 10 0.45403955185425104 1111 11 -0.025816911777702956 1111 12 -0.09600671841845099 1111 13 -0.06934011251546934 1111 14 0.07619569500842971 1111 15 0.10808924728205227 1111 16 0.11579895070087076 1111 17 -0.14939209823300814 1111 18 -0.047439239776913666 1111 19 -0.23195139425861555 1111 20 -0.2853992911033103 1111 21 -0.0069547559501828005 1111 22 0.19183787572301014 1111 23 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15 0.3556534524619437 2202 16 0.38646738615972254 2202 17 0.3233330522131368 2202 18 0.4315483243294551 2202 19 0.5346402963707391 2202 20 0.4145199274660456 2202 21 0.5398398022964415 2202 22 0.6025700716933216 2202 23 0.7781876816948881 2202 24 0.5469201769650027 2202 25 0.5838015868923918 2203 1 0.18673181468247435 2203 2 0.19583837373005153 2203 3 0.20614740774533757 2203 4 -0.21567454098068595 2203 5 0.061447475489316616 2203 6 -0.16161889567313636 2203 7 0.2194597353701937 2203 8 0.17759813381434952 2203 9 0.5229679943544738 2203 10 0.4871623604790827 2203 11 0.2869602011517216 2203 12 0.4456754560746603 2203 13 0.14171138492024937 2203 14 0.5786950794670105 2203 15 0.7195156046701712 2203 16 0.5857996569034327 2203 17 0.6451487651282477 2203 18 0.71442734901661 2203 19 0.5895396856396147 2203 20 0.6933478292126125 2203 21 0.44445755373299534 2203 22 0.34040380736641396 2203 23 0.7886627005223303 2203 24 0.5860309545123812 2203 25 0.49721536238680386 2204 1 0.05733553858693341 2204 2 -0.05576419037251412 2204 3 -0.21367736813078508 2204 4 -0.5416748701136527 2204 5 -0.44576602209703964 2204 6 -0.2955289953534077 2204 7 0.08931899184313608 2204 8 0.070463393045749 2204 9 0.44842133583776445 2204 10 0.1722134544543863 2204 11 -0.14821039943758052 2204 12 0.00896498337702667 2204 13 -0.08080013473368552 2204 14 0.4248528438704966 2204 15 0.04961442292607643 2204 16 0.027239456915971516 2204 17 -0.07438684392447925 2204 18 -0.11764430793279956 2204 19 0.2524977290224797 2204 20 0.009568207245337135 2204 21 0.07462964325716825 2204 22 -0.04475425482404205 2204 23 0.3377564093807095 2204 24 0.025823130551501094 2204 25 0.06260311237112859 2205 1 0.36892447458531663 2205 2 0.4493816808166642 2205 3 0.39255622766973985 2205 4 0.1422376576472999 2205 5 0.08840266354802395 2205 6 0.0211275764212715 2205 7 0.04646112792228507 2205 8 0.12389439445902894 2205 9 0.3281621494860616 2205 10 0.27117368057996316 2205 11 0.17477440774997888 2205 12 0.3163304689413309 2205 13 0.19960946550858694 2205 14 0.4258345721160026 2205 15 0.4781741342121677 2205 16 0.3854781506369491 2205 17 0.6410558170890468 2205 18 0.6757586801552264 2205 19 0.5758156676455357 2205 20 0.5610887503505282 2205 21 0.5885353569660268 2205 22 0.712765177843032 2205 23 1.0018072371618487 2205 24 0.9048913720213179 2205 25 0.8663180326214857 2206 1 0.08557412016396171 2206 2 0.22193200381447298 2206 3 0.44886420260342064 2206 4 0.1685494396645737 2206 5 0.48361661560294067 2206 6 0.20438245160331547 2206 7 0.2980114569630814 2206 8 0.4166977978709028 2206 9 0.5916884757712566 2206 10 0.6516445503474912 2206 11 0.2992007769404026 2206 12 0.3289077436737742 2206 13 0.21912348826649009 2206 14 0.26646219703971497 2206 15 0.21321924513124912 2206 16 0.25088324246017635 2206 17 0.05188364626581432 2206 18 0.13681040210518347 2206 19 0.011541823518413707 2206 20 0.2387145805062545 2206 21 0.3241696593934975 2206 22 0.2799765193263397 2206 23 0.6949025169824949 2206 24 0.37773538432484344 2206 25 0.6821693992436337 2207 1 -0.7874720501624992 2207 2 0.06902092623408862 2207 3 0.3546451951433127 2207 4 0.4189768738074804 2207 5 0.34304502549833416 2207 6 0.4293374144887707 2207 7 0.6535095804683024 2207 8 0.5913705116406689 2207 9 0.860864119898082 2207 10 0.5742991178486108 2207 11 0.5331706017636932 2207 12 0.4322451395428857 2207 13 0.4148232342236895 2207 14 -4.578468787379244 2207 15 -1.6356810805741873 2207 16 -0.46400857547705243 2207 17 -0.4490425127037341 2207 18 0.01019318844266337 2207 19 0.08315796472108161 2207 20 0.49479691566669715 2207 21 0.6865916580867122 2207 22 0.4928982347165771 2207 23 0.9031314456261015 2207 24 0.7746788117577452 2207 25 0.7775693523777605 2208 1 0.407169346754074 2208 2 0.8586470157053618 2208 3 0.7076189281655125 2208 4 0.7341145410924241 2208 5 0.8264324584347733 2208 6 0.5595279847035712 2208 7 0.642678441035919 2208 8 0.6460509998833501 2208 9 0.9426342988007264 2208 10 0.827187338594932 2208 11 0.5955870839432365 2208 12 0.6389804154757925 2208 13 0.4298539488370636 2208 14 1.1081386938230153 2208 15 0.8455249167532415 2208 16 0.7087150029748486 2208 17 0.5646207534164489 2208 18 0.8758267272049025 2208 19 0.5757111668192465 2208 20 0.6049207713478992 2208 21 0.7560161579770153 2208 22 0.8654724783193615 2208 23 1.3887713666456634 2208 24 1.0628601675885392 2208 25 0.8838875378222255 2209 1 -0.2936726764989537 2209 2 -0.023417289700368826 2209 3 0.27863654772682267 2209 4 0.46653243633394226 2209 5 0.6012062208703455 2209 6 0.5187602752465423 2209 7 0.6936568061324095 2209 8 0.6224533731303836 2209 9 0.8570985203037864 2209 10 0.7155293618479632 2209 11 0.270284435015995 2209 12 0.37354831671035527 2209 13 0.44856724141767046 2209 14 0.5185950644331072 2209 15 0.5173530960652357 2209 16 0.749894734486297 2209 17 0.850541585251527 2209 18 0.7813163592317522 2209 19 0.5640097404738071 2209 20 0.7084806418980634 2209 21 0.8236027575215417 2209 22 0.7694338830898246 2209 23 0.8609935888678848 2209 24 0.7721823538524717 2209 25 0.8636984571557988 2210 1 -0.3306971983422393 2210 2 -0.4646030524159599 2210 3 0.17349014743231805 2210 4 0.43795841750987563 2210 5 0.9131784078363552 2210 6 0.6396936767113672 2210 7 0.8051726769243288 2210 8 0.9016867289910664 2210 9 1.2915700341965675 2210 10 0.9884056097806635 2210 11 0.7548861679735026 2210 12 0.7416864038637243 2210 13 0.954065837579229 2210 14 1.1474460048125508 2210 15 1.0387733080420958 2210 16 1.0355964613998836 2210 17 1.0701241699625967 2210 18 0.8007255932469519 2210 19 0.8921425529308848 2210 20 1.0333305053603539 2210 21 1.162289083420852 2210 22 1.1991306979424934 2210 23 1.1090128305204061 2210 24 1.0390517666578916 2210 25 0.8460474552382209 2211 1 -1.1793906981393043 2211 2 -0.3083703804787028 2211 3 0.4264741470207305 2211 4 0.4050601078713214 2211 5 0.7682815044504849 2211 6 0.4875582877338758 2211 7 0.5672715947957414 2211 8 0.6607816396139938 2211 9 0.7940652752317092 2211 10 0.9724595047004314 2211 11 0.6474950525178684 2211 12 0.7733799665420094 2211 13 0.6460436233600717 2211 14 0.9882593447769992 2211 15 0.6082213252829831 2211 16 0.6501431735696883 2211 17 0.797715590677953 2211 18 0.9931106778221874 2211 19 0.9470635798383285 2211 20 1.0695672359943629 2211 21 0.8302305238580487 2211 22 1.077874274054487 2211 23 1.4687376966119956 2211 24 1.1104190901474595 2211 25 1.184462734230749 2212 1 0.9613340385634928 2212 2 0.18578352912310336 2212 3 0.5775322601446575 2212 4 0.7965496529257692 2212 5 0.8606694300193993 2212 6 0.7034866142654934 2212 7 0.8297270450939678 2212 8 0.8309738111772802 2212 9 1.138644522918817 2212 10 0.6815768820079384 2212 11 0.7820136079687856 2212 12 0.904322235052831 2212 13 0.5866209382518264 2212 14 0.8839684159888108 2212 15 0.9509893467530119 2212 16 0.2550804192747549 2212 17 0.946376175810208 2212 18 0.9213387230520595 2212 19 0.8719707871952593 2212 20 0.8377056586117675 2212 21 0.9561453700653999 2212 22 1.092953463265303 2212 23 1.3922245456015858 2212 24 0.9472887003260269 2212 25 0.5753767494592097 2213 1 0.36690884816024294 2213 2 0.2469966748883543 2213 3 -0.15463618408960045 2213 4 -0.9385074856962703 2213 5 -1.8048208186518373 2213 6 -0.3668090666334791 2213 7 -0.05708977956562933 2213 8 0.08969106852342179 2213 9 -0.5899876088211755 2213 10 -0.35657082432989284 2213 11 -0.14603549101267532 2213 12 -0.5228480796860353 2213 13 -0.6165793892629452 2213 14 -7.693272836755185 2213 15 -3.200184483122829 2213 16 -1.818416294989781 2213 17 -1.1315903386279729 2213 18 -5.334466899755391 2213 19 -2.9046680218932757 2213 20 -5.086842420415291 2213 21 -2.133951190323664 2213 22 -1.7024645062842936 2213 23 -4.897371919497623 2213 24 -2.434440704712337 2213 25 -1.9560089062408892 2214 1 0.22917111042250227 2214 2 0.1894891673808452 2214 3 -1.7169842464376615 2214 4 -2.188350185538969 2214 5 -0.3100975239285404 2214 6 -0.40436090418531734 2214 7 -0.5844600069358563 2214 8 -0.7331818043494494 2214 9 -5.239508558342127 2214 10 -1.5790075467666185 2214 11 -0.7947127396899205 2214 12 -1.2259225827605391 2214 13 -1.3146345873181389 2214 14 -2.5078445513268974 2214 15 -1.7901673231056705 2214 16 -1.1599578365313263 2214 17 -1.6195282765659087 2214 18 -0.9047729200614114 2214 19 -4.148318815544069 2214 20 -1.912010445583313 2214 21 -3.629124935497412 2214 22 -5.828354682174467 2214 23 -6.465196887322588 2214 24 -5.51901813928977 2214 25 -3.326936977168959 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22327.0 3 7.0 4 413.0 5 2583.0 6 3106.0 7 2608.0 8 1889.0 9 1561.0 10 1405.0 11 1341.0 12 1244.0 13 1260.0 14 1274.0 15 1247.0 16 1321.0 17 1383.0 18 1610.0 19 1868.0 20 2339.0 21 3194.0 22 4039.0 23 5113.0 24 6061.0 25 7147.0 26 8272.0 27 9311.0 28 10858.0 29 13014.0 30 16247.0 31 20348.0 32 26548.0 33 34489.0 34 45370.0 35 59647.0 36 81595.0 37 119884.0 38 184681.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.43722158877644 19.39519698589973 9.187056932218155 29.98052449310567 2 14.018104134164261 21.489952686881157 38.344861953853425 26.147081225101154 3 18.474020090827757 24.828282313502985 28.93223592566167 27.765461670007586 4 12.582177702025493 17.577251286813627 36.31093607551571 33.52963493564516 5 13.694208909587646 38.162875302577746 34.45620939088091 13.686706396953696 6 32.42275780660722 37.30083083920584 17.257136850538433 13.019274503648504 7 28.391516564424137 31.742830969417994 21.34599949516865 18.519652970989213 8 25.719367067603272 37.43942340011828 19.493574661146877 17.34763487113157 9 26.529573117977606 15.570315403097016 19.525264301498016 38.37484717742736 10 15.295016879339265 29.753214859480387 33.01655378387369 21.935214477306666 11 34.550974051246484 23.006856924503065 23.43256649943036 19.009602524820085 12 23.35385952274133 25.00930542601868 30.961239893175126 20.67559515806487 13 29.125365834328704 22.209186475027032 25.1052923561146 23.560155334529668 14 21.310874100174335 22.379972497164037 26.854635757368904 29.45451764529272 15 23.346636663411598 30.766639776956932 22.861631214707256 23.025092344924214 16 22.59959127525517 28.208800602331202 26.976540784728865 22.21506733768476 17 21.979549233980823 28.554081307716807 27.358171460920637 22.108197997381737 18 21.947046185983712 27.73989566615434 28.884143010396592 21.428915137465363 19 23.640192530594163 27.02009230259741 26.9098443366653 22.429870830143127 20 23.60037305458966 27.583537480239855 27.388943375238288 21.427146089932197 21 23.017625197777534 26.77900713555863 26.853730954625092 23.349636712038745 22 23.370940441888244 27.86355582476182 26.493056931463734 22.272446801886208 23 22.5325960971012 27.806561817597718 27.025359360206508 22.635482725094572 24 23.25549141455669 27.14621930560725 27.620884711782995 21.977404568053068 25 22.926136846097673 27.072164890094026 27.332565340700025 22.669132923108275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 193.0 1 193.0 2 226.0 3 259.0 4 259.0 5 259.0 6 377.0 7 495.0 8 495.0 9 495.0 10 899.5 11 1304.0 12 1304.0 13 1304.0 14 2053.0 15 2802.0 16 2802.0 17 2802.0 18 5069.0 19 7336.0 20 7336.0 21 7336.0 22 11458.5 23 15581.0 24 15581.0 25 15581.0 26 22406.5 27 29232.0 28 29232.0 29 29232.0 30 38619.5 31 48007.0 32 48007.0 33 48007.0 34 55395.0 35 62783.0 36 62783.0 37 62783.0 38 69258.0 39 75733.0 40 75733.0 41 75733.0 42 80822.0 43 85911.0 44 85911.0 45 85911.0 46 90435.5 47 94960.0 48 94960.0 49 94960.0 50 92104.5 51 89249.0 52 89249.0 53 89249.0 54 80294.0 55 71339.0 56 71339.0 57 71339.0 58 62085.0 59 52831.0 60 52831.0 61 52831.0 62 43853.5 63 34876.0 64 34876.0 65 34876.0 66 27278.0 67 19680.0 68 19680.0 69 19680.0 70 14292.0 71 8904.0 72 8904.0 73 8904.0 74 6184.0 75 3464.0 76 3464.0 77 3464.0 78 2329.0 79 1194.0 80 1194.0 81 1194.0 82 779.5 83 365.0 84 365.0 85 365.0 86 226.5 87 88.0 88 88.0 89 88.0 90 51.5 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08335644859072408 2 0.03481440807014961 3 0.030002660613299672 4 0.029436572677199672 5 0.02462482522034973 6 0.3703630321934209 7 0.20067817334744778 8 0.21299058595762266 9 0.2176608114304476 10 0.016841116098974814 11 0.0026889176964749704 12 0.003821093568674958 13 0.0 14 0.07005338209237423 15 0.0019813077763499785 16 0.002264351744399975 17 0.004104137536724954 18 0.003679571584649959 19 0.0021228297603749766 20 7.076099201249923E-4 21 2.830439680499969E-4 22 0.0 23 1.4152198402499845E-4 24 1.4152198402499845E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 706604.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.8566762824363 #Duplication Level Percentage of deduplicated Percentage of total 1 90.2922368297618 72.1043792733136 2 6.817054150355621 10.887745729695753 3 1.4080486688179807 3.3732626020713856 4 0.4995543141280525 1.5957098859527352 5 0.26880014385642587 1.0732743036307453 6 0.1570967932608931 0.7527136658666376 7 0.10911878341745347 0.6099704354590602 8 0.07361709546667522 0.4703053249228393 9 0.04930772361785337 0.354379583085728 >10 0.292197825585435 4.101822137863826 >50 0.016751501181160856 0.8881532622388943 >100 0.013899574762074023 2.085470082293585 >500 0.0014255974082634994 0.8111347290280464 >1k 8.909983801646872E-4 0.8916789845771471 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1618 0.2289825701524475 No Hit CCCATGTACTCTGCGTTGATACCAC 1307 0.18496923312067295 No Hit GTACATGGGAAGCAGTGGTATCAAC 1257 0.17789313391942305 No Hit CTGTAGGACGTGGAATATGGCAAGA 1058 0.14973025909844837 No Hit GTCCTACAGTGGACATTTCTAAATT 1026 0.1452015556096484 No Hit GGTATCAACGCAGAGTACTTTTTTT 904 0.1279358735585986 No Hit CTTTAGGACGTGAAATATGGCGAGG 828 0.11718020277269872 No Hit GTCCTAAAGTGTGTATTTCTCATTT 770 0.10897192769924881 No Hit TATCAACGCAGAGTACTTTTTTTTT 744 0.10529235611459885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 1.4152198402499845E-4 0.0 0.0 0.0 0.0 10 1.4152198402499845E-4 0.0 0.0 0.0 0.0 11 1.4152198402499845E-4 0.0 0.0 0.0 0.0 12 1.4152198402499845E-4 0.0 0.0 0.0 1.4152198402499845E-4 13 1.4152198402499845E-4 0.0 0.0 0.0 1.4152198402499845E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 35 0.002204844 16.245377 19 TAGGACC 310 0.0 11.993303 4 GATATAC 180 0.0 11.64581 1 AATCCCG 75 2.119007E-4 11.371764 19 CCTTTAA 110 4.765352E-7 11.260825 1 CACTATG 85 5.309227E-5 11.177263 7 CTCTAAG 60 0.005741276 11.116456 1 CCATTAT 60 0.005741276 11.116456 1 TGTAGGA 740 0.0 11.082998 2 TGGCGAG 545 0.0 10.954452 18 TATAACT 70 0.0014469854 10.895016 4 GTGTAAC 80 3.662729E-4 10.7194395 1 GACGTGA 295 0.0 10.627872 7 TAAGGCT 90 9.1676695E-5 10.592377 4 GTAGGAC 730 0.0 10.580126 3 TAGGACT 145 1.3604222E-8 10.519325 4 GTGCTAA 75 0.0025798222 10.163616 1 GGACCTG 460 0.0 10.1534195 6 CTGTAGG 770 0.0 10.147118 1 ATAATAC 85 6.363412E-4 10.096067 3 >>END_MODULE