FastQCFastQC Report
Thu 26 May 2016
SRR1926612_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926612_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759508
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG350.00593204419.0512961
GTACTAA500.002435435816.003091
GGTCTAC500.002435435816.003091
AGGACCG400.002824555215.6525095
GTTCTAG604.5648994E-415.558561
TAGGACA1052.1645974E-1015.3351014
GCTTAGG708.493044E-515.2410371
GTATAGA708.493044E-515.2410371
TTAGGAC951.816934E-713.8495563
GTATATA1551.9645086E-1013.76611
TAGGACT1202.3230314E-812.5236654
AGGATTG600.0029105712.1741735
GCACTGT600.00291652412.17085556
TGTAGGA2251.8189894E-1212.1563552
CTGTAGG1903.8380676E-1011.9321291
GTCCTAA1256.5939585E-611.73561
GTCTTAG800.00399435911.668921
GTAGGAC1659.549694E-1011.2964813
GTATCAA4850.011.2736541
GGAGTGC650.005303970511.2346346
Produced by FastQC (version 0.11.5)