##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926612_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 759508 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.529646823996586 27.0 2.0 30.0 2.0 31.0 2 15.76649883872191 16.0 2.0 30.0 2.0 31.0 3 19.081680508961064 19.0 10.0 30.0 2.0 31.0 4 21.409494040879096 27.0 2.0 35.0 2.0 35.0 5 21.525300589328882 28.0 2.0 35.0 2.0 35.0 6 22.25333505374532 29.0 2.0 35.0 2.0 36.0 7 22.225266883298136 30.0 2.0 35.0 2.0 37.0 8 22.594761345502615 30.0 2.0 35.0 2.0 37.0 9 25.20580955039315 31.0 17.0 37.0 2.0 39.0 10 25.94064578648283 31.0 17.0 37.0 2.0 39.0 11 23.129404825228963 30.0 7.0 37.0 2.0 39.0 12 24.27037634889955 30.0 7.0 37.0 2.0 39.0 13 20.992023783817945 27.0 2.0 35.0 2.0 39.0 14 24.20473385402129 30.0 8.0 37.0 2.0 40.0 15 25.29112399079404 31.0 8.0 38.0 2.0 40.0 16 25.605994933562254 31.0 7.0 38.0 2.0 40.0 17 24.904218257082217 30.0 2.0 38.0 2.0 40.0 18 24.989044223365653 30.0 7.0 38.0 2.0 40.0 19 25.389263839222235 31.0 2.0 38.0 2.0 40.0 20 25.66362566292916 31.0 2.0 38.0 2.0 40.0 21 23.546535388699 30.0 2.0 37.0 2.0 40.0 22 24.43198886647672 30.0 2.0 38.0 2.0 40.0 23 24.38630402839733 31.0 2.0 38.0 2.0 40.0 24 23.501469372277842 30.0 2.0 38.0 2.0 40.0 25 23.278182718286047 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.325434272239427 1101 2 9.58867710988566 1101 3 6.1571064957179935 1101 4 7.90369090679749 1101 5 7.039942572487504 1101 6 6.752128513810842 1101 7 6.480705077891383 1101 8 5.103606598717011 1101 9 4.97010114509747 1101 10 4.663852394345856 1101 11 7.457393459544402 1101 12 5.120156611394307 1101 13 9.5474855959825 1101 14 6.688551808986588 1101 15 6.0567154622707235 1101 16 5.539660647702334 1101 17 1.7893082280989532 1101 18 4.087103010884469 1101 19 4.437398249980372 1101 20 2.904707474199906 1101 21 0.10055161665988166 1101 22 3.5514761288659074 1101 23 4.544631325146145 1101 24 5.913749503410795 1101 25 6.376271292357966 1102 1 5.449653179368262 1102 2 10.228885447553582 1102 3 6.570204929024278 1102 4 8.214662964305344 1102 5 7.622754021349763 1102 6 6.295989349242117 1102 7 6.170620744134993 1102 8 6.632012024928443 1102 9 5.638794665407616 1102 10 5.012889075368683 1102 11 7.649433074216809 1102 12 4.906260267012975 1102 13 9.519726890394697 1102 14 7.026393114495331 1102 15 4.907529324631927 1102 16 5.415195535939301 1102 17 6.24430479510476 1102 18 6.251200133411153 1102 19 5.284428883385139 1102 20 5.533781120594568 1102 21 4.570925570290189 1102 22 5.444826176372999 1102 23 5.878498679798717 1102 24 7.30604537896302 1102 25 6.9536887791288 1103 1 4.905700514091034 1103 2 8.927821894914592 1103 3 6.036178402991119 1103 4 7.810057639383732 1103 5 6.633587284272462 1103 6 6.24525731334208 1103 7 6.36101755500264 1103 8 6.008400874700314 1103 9 4.979355063650168 1103 10 4.458890572768183 1103 11 5.111130275628778 1103 12 4.0013228385971225 1103 13 7.977036335746654 1103 14 6.37118108460367 1103 15 5.46945899848987 1103 16 5.353186063361896 1103 17 2.837553119331652 1103 18 1.7386699741892393 1103 19 3.7670327625417173 1103 20 4.345679696422128 1103 21 6.564181404984794 1103 22 2.36719199717324 1103 23 4.61532778379992 1103 24 6.0495552786982785 1103 25 2.2736129938155187 1104 1 5.435567609169169 1104 2 9.752156457496744 1104 3 6.49283368997812 1104 4 6.925673967702707 1104 5 6.856280617118962 1104 6 6.412310402286142 1104 7 6.396128735081508 1104 8 6.517515267865203 1104 9 5.4414872018128655 1104 10 4.060545494631771 1104 11 7.450252336385315 1104 12 4.518346717668248 1104 13 8.727789402783312 1104 14 6.30387971173964 1104 15 5.666068827612111 1104 16 5.101734697800335 1104 17 5.4409104291023525 1104 18 3.2845796791695214 1104 19 4.1117275193695235 1104 20 3.327528498817337 1104 21 2.2835931911685243 1104 22 4.40937744335405 1104 23 4.601704116500372 1104 24 5.804890644551936 1104 25 5.095985018059714 1105 1 3.2312037465329944 1105 2 9.059002103554027 1105 3 5.860772511130996 1105 4 4.995280237091638 1105 5 1.530433816743809 1105 6 4.224264806730268 1105 7 5.359465615537463 1105 8 2.669132214694436 1105 9 3.463495895269329 1105 10 3.4394683717690633 1105 11 6.490933023204445 1105 12 3.8325195767693216 1105 13 5.061878754200961 1105 14 3.444517111638639 1105 15 3.877426233282698 1105 16 4.162453621398921 1105 17 4.822786736728066 1105 18 2.2310572060434843 1105 19 2.1437959127033395 1105 20 1.4281345156992344 1105 21 2.1475820808590207 1105 22 3.429370589591695 1105 23 0.6310400230006259 1105 24 3.7529328132266357 1105 25 4.460203869513432 1106 1 6.352873959873289 1106 2 10.945446874422414 1106 3 7.754515144097514 1106 4 9.14390479506963 1106 5 8.568208702695383 1106 6 7.551609660379366 1106 7 7.52122823734658 1106 8 7.047757807431328 1106 9 6.043551929713303 1106 10 5.543776567959352 1106 11 8.059375576089625 1106 12 5.311320516150445 1106 13 4.929711361703411 1106 14 5.8899234569601795 1106 15 5.901501248804081 1106 16 5.816456113818187 1106 17 6.037587500650073 1106 18 6.263690352520356 1106 19 6.0298790803252835 1106 20 5.570917747206295 1106 21 5.439947584696629 1106 22 6.35433144871152 1106 23 6.617212494814694 1106 24 7.471806253215117 1106 25 6.689520501238242 1107 1 3.9639981108032636 1107 2 -13.309220788012238 1107 3 -5.157629669018172 1107 4 2.1711248242314056 1107 5 4.476519809064744 1107 6 4.8155166271989565 1107 7 4.704734051920354 1107 8 5.366318211428627 1107 9 3.768904893901219 1107 10 3.107516388009852 1107 11 5.8878260899770325 1107 12 3.1610733523110497 1107 13 4.505037653534558 1107 14 4.041770962205742 1107 15 3.825794431349692 1107 16 3.5504153584570446 1107 17 4.014919553710282 1107 18 -0.699096375314916 1107 19 2.1507368633189863 1107 20 2.503852773345205 1107 21 -0.9448811787729134 1107 22 2.130625058014836 1107 23 -0.991408010893192 1107 24 2.4114840511453437 1107 25 3.2165164826031543 1108 1 5.212781078505152 1108 2 10.36353304690376 1108 3 0.2680755729032853 1108 4 5.9317527524269025 1108 5 7.351524424609899 1108 6 7.1849464668990635 1108 7 7.378298617376814 1108 8 6.987159070107321 1108 9 5.461802305717548 1108 10 4.532795661937772 1108 11 6.709402225066679 1108 12 2.51959659191537 1108 13 3.3882046934583556 1108 14 5.037874104254829 1108 15 4.69797834677685 1108 16 4.721261167140689 1108 17 0.21480128872715198 1108 18 3.5368230011450024 1108 19 4.541569989287247 1108 20 4.634234298321324 1108 21 4.744472564634886 1108 22 1.8120272204980807 1108 23 4.466877896041364 1108 24 5.167198898752083 1108 25 5.5964847490579075 1109 1 4.325689846963087 1109 2 -13.309220788012238 1109 3 -4.889679652131985 1109 4 2.7446542105267504 1109 5 2.604847903350283 1109 6 3.1622567271305435 1109 7 4.464673088455136 1109 8 4.574082393660891 1109 9 4.20717332365901 1109 10 3.27065600646862 1109 11 1.3813599792556026 1109 12 1.898946039119906 1109 13 3.222660771157674 1109 14 4.044162739065605 1109 15 4.178454222307355 1109 16 3.926845705100156 1109 17 4.7766892261182505 1109 18 4.596122668840298 1109 19 3.2340992062557987 1109 20 2.1218075530021103 1109 21 1.285688881584381 1109 22 3.663998225430557 1109 23 4.068921572840647 1109 24 5.184717279697718 1109 25 5.417275062085139 1110 1 3.116173770745135 1110 2 8.898945745214343 1110 3 5.992951748496793 1110 4 7.368331087410446 1110 5 8.08135708547928 1110 6 3.9182115303742435 1110 7 6.345897532155092 1110 8 7.153345488928281 1110 9 5.376815104853861 1110 10 5.023439162859766 1110 11 2.7137715349777203 1110 12 5.612049224583959 1110 13 9.693496699159336 1110 14 7.402626973021722 1110 15 6.781299934653433 1110 16 6.513786494926659 1110 17 6.932049448101985 1110 18 6.80031502193475 1110 19 5.638754530199744 1110 20 6.080092226446148 1110 21 4.071105236733118 1110 22 6.116736144686371 1110 23 6.636767496545726 1110 24 7.4718241913894055 1110 25 7.808894267615049 1111 1 4.951888747470772 1111 2 9.260831102276866 1111 3 7.311486784086419 1111 4 7.96341649598935 1111 5 8.307116343738372 1111 6 7.541982303849952 1111 7 7.2062614348413625 1111 8 7.198990900735858 1111 9 6.027388046528181 1111 10 5.350044702295023 1111 11 7.25109449935367 1111 12 6.424769971975053 1111 13 8.192789535949153 1111 14 5.950843752523898 1111 15 5.287559352862573 1111 16 5.229769214386142 1111 17 6.476333652337129 1111 18 6.380299840033857 1111 19 5.217303062131293 1111 20 5.050947487378391 1111 21 7.539995693798549 1111 22 6.901300935139936 1111 23 6.741302421743111 1111 24 6.6062249662717285 1111 25 7.170774283117442 1112 1 -2.1146958728087526 1112 2 7.4575452328521425 1112 3 5.778812031357955 1112 4 -0.02781175831173499 1112 5 3.8089955300650544 1112 6 2.774488802564573 1112 7 5.700164850629843 1112 8 5.815570052483746 1112 9 4.382120094001664 1112 10 3.1545043727027533 1112 11 -0.7790829572598845 1112 12 3.593297412403963 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4.157847341360796 2204 9 2.6763520728312855 2204 10 2.2709448480162564 2204 11 3.8287222027362624 2204 12 3.95443537685178 2204 13 2.017816383043325 2204 14 3.0879192372513558 2204 15 3.3400550756483582 2204 16 3.3336101104730886 2204 17 -1.670613670986441 2204 18 1.8106600279776082 2204 19 2.5407506518118623 2204 20 2.9858650576388577 2204 21 4.527312027260599 2204 22 3.9363326583992446 2204 23 2.845536793351993 2204 24 4.368388743411252 2204 25 4.365028300316492 2205 1 5.706815653620808 2205 2 10.069908341116891 2205 3 6.759208597820095 2205 4 7.577865080971662 2205 5 7.518921551466484 2205 6 6.983359745042073 2205 7 7.091816189002493 2205 8 6.85210509721551 2205 9 5.8574885324848545 2205 10 5.480669211162674 2205 11 7.4371231892741285 2205 12 6.650937392175091 2205 13 9.663851962348865 2205 14 7.193807064241845 2205 15 6.131039786595046 2205 16 5.862483470525156 2205 17 6.386155074945801 2205 18 6.250015923797385 2205 19 5.112323174905299 2205 20 5.38068345017588 2205 21 -0.6854844193761522 2205 22 3.846521173910954 2205 23 4.967303997818817 2205 24 6.6299657196270125 2205 25 7.193088109174781 2206 1 4.485621304909678 2206 2 8.82351597559701 2206 3 6.147558356975225 2206 4 2.1407014072308073 2206 5 5.857364020781436 2206 6 6.3095533883498085 2206 7 6.208343790429424 2206 8 6.265209476762845 2206 9 4.955736108920352 2206 10 4.15953431788013 2206 11 3.49899210516989 2206 12 5.018709526725747 2206 13 8.151320974985666 2206 14 0.3065053329803824 2206 15 2.313401678688482 2206 16 3.247412023238944 2206 17 4.399089149960414 2206 18 -0.9696718848165986 2206 19 1.1788477531748427 2206 20 2.860337600970972 2206 21 3.788637353403338 2206 22 -1.7180232362710406 2206 23 -2.6814605385296133 2206 24 1.2538093619270292 2206 25 2.808567682708045 2207 1 1.5961651257923641 2207 2 -13.309220788012238 2207 3 -5.729182400540228 2207 4 -4.134334849019677 2207 5 1.2213893566950276 2207 6 1.8541062584266221 2207 7 3.1294458431965637 2207 8 3.0331511739671093 2207 9 0.8455594254637262 2207 10 0.5159429818658943 2207 11 -1.0189993443023901 2207 12 -2.0032129237850427 2207 13 0.3510122821778978 2207 14 -5.163861186831316 2207 15 -1.3080641946868496 2207 16 -0.1409501778471416 2207 17 1.0709157558721252 2207 18 0.9070402794413717 2207 19 0.5368404836250598 2207 20 0.026469573722742723 2207 21 -3.6492986937732503 2207 22 -3.819760905714684 2207 23 -1.23968212941271 2207 24 0.23893972430653676 2207 25 0.9393366583374423 2208 1 4.726397378202531 2208 2 6.235779211987763 2208 3 6.061417950029448 2208 4 7.861747534854118 2208 5 3.151135193680126 2208 6 6.033593550275878 2208 7 6.422920865107169 2208 8 6.434102094212509 2208 9 4.831615516611841 2208 10 4.417205032698497 2208 11 6.312258324409264 2208 12 5.849223535461235 2208 13 8.418242781739686 2208 14 2.615928471363251 2208 15 4.384310914866173 2208 16 4.547393380435064 2208 17 5.730157648948374 2208 18 0.6385959323773918 2208 19 3.0083742259563486 2208 20 4.094786839279269 2208 21 6.695723216831482 2208 22 5.723372310762091 2208 23 2.10122935174417 2208 24 3.0256049302662227 2208 25 4.437798533885207 2209 1 2.7547858799446914 2209 2 4.2776694558902 2209 3 4.8143796225033135 2209 4 0.058533249139831156 2209 5 4.541361674516363 2209 6 5.201812191390861 2209 7 5.57976755500264 2209 8 4.2385838015295825 2209 9 3.9267636002355353 2209 10 3.126573499597452 2209 11 -0.310363626810247 2209 12 2.718548448353218 2209 13 2.1401460918442154 2209 14 3.468742060213426 2209 15 3.710312657026453 2209 16 3.3105031365326276 2209 17 0.8009677534779911 2209 18 2.876627291262409 2209 19 3.6267888601026925 2209 20 3.7001004281294456 2209 21 5.824852136692112 2209 22 4.524966387417141 2209 23 -0.10647099668788584 2209 24 -2.987792282277333 2209 25 -0.39483212813570034 2210 1 -2.80696474006351 2210 2 -13.309220788012238 2210 3 -7.652364504505609 2210 4 -2.666897095135589 2210 5 -1.4854963973069495 2210 6 -2.0177372286329636 2210 7 -1.835847864161959 2210 8 -20.15089790578749 2210 9 -12.964257753432767 2210 10 -7.581044424009729 2210 11 -9.882705646767675 2210 12 -5.328703377497707 2210 13 -5.248056543434739 2210 14 -4.350775749156021 2210 15 -6.215497503296927 2210 16 -5.563875634777105 2210 17 -5.211265203619394 2210 18 -5.236514441636103 2210 19 -10.234744268839487 2210 20 -7.339443402056656 2210 21 -5.660680191609554 2210 22 -9.996954808075838 2210 23 -7.972519933398228 2210 24 -10.509761076825166 2210 25 -8.324012057445001 2211 1 -0.14475932561374805 2211 2 4.387390717109904 2211 3 0.2986576213116727 2211 4 1.2111632727804924 2211 5 0.5228936086300315 2211 6 -0.054449340639354915 2211 7 -19.767793420607116 2211 8 -12.971228875054631 2211 9 -8.981951971849265 2211 10 -7.127295136163376 2211 11 -9.300279106638804 2211 12 -4.9718183422827735 2211 13 -6.6433754778572975 2211 14 -4.89752362815268 2211 15 -5.4235946350606525 2211 16 -11.345021907192988 2211 17 -8.145296589484587 2211 18 -6.132797740931174 2211 19 -8.583867696817489 2211 20 -6.900257064807185 2211 21 -4.840394648754469 2211 22 -6.380919032252656 2211 23 -8.109959435827594 2211 24 -21.195109355448064 2211 25 -20.98934432325765 2212 1 -0.7230204582962294 2212 2 -13.309220788012238 2212 3 -7.283916484322329 2212 4 -19.00396675086017 2212 5 -19.053150520605588 2212 6 -19.747120735438408 2212 7 -9.32766129839407 2212 8 -8.9865709033102 2212 9 -4.768417513941422 2212 10 -5.062163257970372 2212 11 -5.226048859736068 2212 12 -2.5936790244066437 2212 13 -5.462050364420513 2212 14 -3.3352812726499614 2212 15 -3.3329681933088864 2212 16 -3.4332218136194363 2212 17 -3.58711733040753 2212 18 -2.7965238033781823 2212 19 -3.2014069466282926 2212 20 -3.3590323184416206 2212 21 -1.1405236865541468 2212 22 -2.4988737668597096 2212 23 -2.612915982690179 2212 24 -1.6930449458692038 2212 25 -2.2106490301114903 2213 1 -18.14003119322604 2213 2 -13.309220788012238 2213 3 -8.27685554118522 2213 4 -5.671524683123984 2213 5 -19.053150520605588 2213 6 -10.19007973722094 2213 7 -19.767793420607116 2213 8 -10.376388101865922 2213 9 -8.22733024351039 2213 10 -7.440028224240642 2213 11 -6.543160570421396 2213 12 -5.389695486693082 2213 13 -18.48104293254603 2213 14 -12.067329741012609 2213 15 -7.362219515487784 2213 16 -6.439727831686145 2213 17 -4.909781235696393 2213 18 -5.066652348753241 2213 19 -5.8967862018511354 2213 20 -5.955958004112631 2213 21 -4.185131124905212 2213 22 -5.044511570062969 2213 23 -4.9630207119176575 2213 24 -4.013291173629881 2213 25 -4.796830954273695 2214 1 -18.14003119322604 2214 2 -13.309220788012238 2214 3 -11.911182496399125 2214 4 -19.00396675086017 2214 5 -19.053150520605588 2214 6 -19.747120735438408 2214 7 -15.779512170607116 2214 8 -14.601093218287492 2214 9 -13.747112161959588 2214 10 -13.655105235158647 2214 11 -14.213874488090735 2214 12 -12.312082268110196 2214 13 -18.48104293254603 2214 14 -16.317319296493892 2214 15 -22.919260513705254 2214 16 -17.738348807064934 2214 17 -22.57555663676591 2214 18 -17.300416344408323 2214 19 -14.78096171154365 2214 20 -13.479515616077872 2214 21 -11.088757809954231 2214 22 -12.146789519595053 2214 23 -12.065907925041543 2214 24 -11.021281230448064 2214 25 -10.76668807325765 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 155780.0 3 2.0 4 810.0 5 2906.0 6 3649.0 7 3404.0 8 2927.0 9 2796.0 10 2748.0 11 2406.0 12 2328.0 13 2572.0 14 3882.0 15 7668.0 16 11138.0 17 12102.0 18 12914.0 19 14787.0 20 17347.0 21 19972.0 22 20610.0 23 22558.0 24 23553.0 25 23465.0 26 19744.0 27 16138.0 28 17591.0 29 19868.0 30 19187.0 31 22660.0 32 28477.0 33 35223.0 34 42058.0 35 47129.0 36 45755.0 37 45285.0 38 28069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.818090659925005 19.9768761888556 13.356051996669684 24.84898115454971 2 14.293001132127115 21.234018553614305 40.56705883328702 23.905921480971557 3 18.441479320285108 26.064447434720456 29.440524947146173 26.053548297848263 4 11.455870613746677 18.125055435085134 39.951492388953966 30.467581562214225 5 13.664159942703453 37.625850495630374 35.5998994262726 13.11009013539357 6 29.58407268740884 37.9368382587476 18.791670924726787 13.687418129116772 7 27.69367441765943 31.960034896160693 22.83622755664457 17.510063129535304 8 24.321809288789996 37.67253597011922 20.807363437717207 17.198291303373576 9 25.99101058431202 16.61381101137517 20.735497647198386 36.65968075711442 10 14.238888213063976 30.066960138064346 34.54442642739791 21.149725221473766 11 31.945325889125286 23.776640209347384 24.69281458721829 19.58521931430904 12 22.592751702646012 26.004900485990518 31.680620411041904 19.72172740032157 13 26.87968682637934 22.24407528502513 25.5632058757816 25.313032012813935 14 20.688538108139944 22.8243234560233 28.53907195407961 27.948066481757138 15 22.310678057390472 30.57166618504364 24.553820355966902 22.56383540159899 16 21.70910759971537 29.409855566841753 27.184965697803896 21.696071135638977 17 21.29644071497645 28.770491692108212 28.53653343026213 21.396534162653207 18 21.012999206859792 28.550907429971605 29.690595069155673 20.74549829401293 19 21.764694872336502 28.187175627220977 28.12447358358891 21.923655916853615 20 22.33809653336573 28.155344933675234 28.734085621403505 20.772472911555536 21 21.813453426786335 26.755449778203094 27.542528884617347 23.88856791039322 22 22.051109724717584 28.054654846025528 28.485478122799364 21.40875730645753 23 21.869768888677996 28.00738649394608 28.339541467966818 21.783303149409107 24 21.385753765051497 28.345438394816597 28.454587150636897 21.814220689495013 25 22.159668351710636 27.756035511148312 28.29911617576305 21.785179961378002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 988.0 1 988.0 2 1961.0 3 2934.0 4 2934.0 5 2934.0 6 4182.5 7 5431.0 8 5431.0 9 5431.0 10 6442.0 11 7453.0 12 7453.0 13 7453.0 14 9383.0 15 11313.0 16 11313.0 17 11313.0 18 15686.5 19 20060.0 20 20060.0 21 20060.0 22 26913.5 23 33767.0 24 33767.0 25 33767.0 26 42776.5 27 51786.0 28 51786.0 29 51786.0 30 60442.5 31 69099.0 32 69099.0 33 69099.0 34 76258.5 35 83418.0 36 83418.0 37 83418.0 38 87186.0 39 90954.0 40 90954.0 41 90954.0 42 91496.0 43 92038.0 44 92038.0 45 92038.0 46 90265.0 47 88492.0 48 88492.0 49 88492.0 50 81262.5 51 74033.0 52 74033.0 53 74033.0 54 63884.0 55 53735.0 56 53735.0 57 53735.0 58 44455.0 59 35175.0 60 35175.0 61 35175.0 62 27910.0 63 20645.0 64 20645.0 65 20645.0 66 15603.0 67 10561.0 68 10561.0 69 10561.0 70 7636.0 71 4711.0 72 4711.0 73 4711.0 74 3301.0 75 1891.0 76 1891.0 77 1891.0 78 1291.5 79 692.0 80 692.0 81 692.0 82 454.5 83 217.0 84 217.0 85 217.0 86 153.0 87 89.0 88 89.0 89 89.0 90 55.0 91 21.0 92 21.0 93 21.0 94 12.5 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.339161667816535 2 36.50126134286933 3 5.774132728029198 4 13.902421040989694 5 13.597355129899883 6 14.96679429314767 7 9.29641294100918 8 7.749358795430726 9 0.11033458502082928 10 1.5076865549803293 11 5.811920348436093 12 5.909746836109693 13 16.319775433570154 14 1.6426423421478116 15 2.9515159813984844 16 3.043022588307167 17 2.9225498612259515 18 1.5598255712908884 19 1.517561368675511 20 0.2862379329776645 21 4.5154231423500475 22 1.6544921185820294 23 0.10875461482960022 24 9.287591440774817 25 0.11494283141191403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 759508.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.80142615818524 #Duplication Level Percentage of deduplicated Percentage of total 1 97.79126180980143 93.6854234718745 2 1.7251046284032023 3.3053496734622594 3 0.24262333095947322 0.6973098337550072 4 0.09458293668718913 0.36244720899448235 5 0.040104599635245194 0.19210389202797623 6 0.026523244978251738 0.15245788171556646 7 0.016771958536788922 0.11247442831032299 8 0.008903464038820655 0.06823716421337077 9 0.00896060044181194 0.07725944714033237 >10 0.03972897577216169 0.688841302712452 >50 0.0029264593182916883 0.18730164847276945 >100 0.0023686585795812667 0.3969780283766617 >500 1.3933284773177452E-4 0.07381601894432051 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.3166418260242158E-4 0.0 6 0.0 0.0 0.0 1.3166418260242158E-4 0.0 7 0.0 0.0 0.0 1.3166418260242158E-4 0.0 8 0.0 0.0 0.0 1.3166418260242158E-4 0.0 9 0.0 0.0 0.0 1.3166418260242158E-4 0.0 10 0.0 0.0 0.0 1.3166418260242158E-4 0.0 11 0.0 0.0 0.0 1.3166418260242158E-4 0.0 12 0.0 0.0 0.0 1.3166418260242158E-4 0.0 13 0.0 0.0 0.0 1.3166418260242158E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCTAG 35 0.005932044 19.051296 1 GTACTAA 50 0.0024354358 16.00309 1 GGTCTAC 50 0.0024354358 16.00309 1 AGGACCG 40 0.0028245552 15.652509 5 GTTCTAG 60 4.5648994E-4 15.55856 1 TAGGACA 105 2.1645974E-10 15.335101 4 GCTTAGG 70 8.493044E-5 15.241037 1 GTATAGA 70 8.493044E-5 15.241037 1 TTAGGAC 95 1.816934E-7 13.849556 3 GTATATA 155 1.9645086E-10 13.7661 1 TAGGACT 120 2.3230314E-8 12.523665 4 AGGATTG 60 0.00291057 12.174173 5 GCACTGT 60 0.002916524 12.1708555 6 TGTAGGA 225 1.8189894E-12 12.156355 2 CTGTAGG 190 3.8380676E-10 11.932129 1 GTCCTAA 125 6.5939585E-6 11.7356 1 GTCTTAG 80 0.003994359 11.66892 1 GTAGGAC 165 9.549694E-10 11.296481 3 GTATCAA 485 0.0 11.273654 1 GGAGTGC 65 0.0053039705 11.234634 6 >>END_MODULE