FastQCFastQC Report
Thu 26 May 2016
SRR1926612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759508
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22480.2959810824902437No Hit
GGTATCAACGCAGAGTACTTTTTTT13250.1744550419482086No Hit
TATCAACGCAGAGTACTTTTTTTTT10190.13416580207186757No Hit
GTACATGGGAAGCAGTGGTATCAAC9220.12139437635943268No Hit
CCCATGTACTCTGCGTTGATACCAC9020.11876109270738426No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7720.10164474896906944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT1101.7462298E-1013.8741014
TAGACTG1257.2759576E-1213.7353595
GACGTGG1001.0011718E-813.3026817
TTAGACA751.41615365E-512.7179244
GGACGTG2200.012.1398386
TGGCGAG2700.011.92992718
GGCGAGG1353.8744474E-1011.92992719
AGACTGG907.118857E-611.6580976
GTAGGAC2950.011.6416793
ATGGGTG1251.7193088E-811.4461335
GCACTAA600.00569703911.1281846
CCAAGAC951.296176E-511.0459793
GTCCTAC1657.4578566E-1110.9807511
TAGACAG1053.2945536E-610.9010785
AGCACTA700.001440497910.9010785
CCCTAAT700.001443688610.898191
ATGGCGA3100.010.69618717
TGTAGGA3400.010.6613212
GGCGAGA1701.4551915E-1010.58832619
TATGGCG3150.010.52640716
Produced by FastQC (version 0.11.5)