FastQCFastQC Report
Thu 26 May 2016
SRR1926611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700619
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCG200.009266444526.5922641
GTCCTAC2450.015.195581
TCTTATA903.900617E-513.6259432
GTATTAC700.001499446513.2961311
GCTCTAT854.5195105E-412.5140071
GTATGGG854.5195105E-412.5140071
GTTTAGG750.002518978412.4097231
TCCTACA3450.012.24362
TGTAGGA2800.012.166022
AGGACGT2150.012.0906295
GGCCTCG556.610161E-411.9633215
TAGGACG2250.011.8956654
GTAGGAC2600.011.7588193
TTAGGAC1502.757588E-911.6468313
GTTAAAT800.004083652511.6341161
GGCGAGA1256.166374E-1011.60022119
CTGTAGG2900.011.4621831
GCTACAC1058.775489E-611.4388513
GGACGTG2100.011.3815596
TAGTACA852.179659E-411.3358694
Produced by FastQC (version 0.11.5)