FastQCFastQC Report
Thu 26 May 2016
SRR1926611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700619
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13730.1959695640569268No Hit
CCCATGTACTCTGCGTTGATACCAC8680.12389044544895299No Hit
GTACATGGGAAGCAGTGGTATCAAC8570.1223204052416506No Hit
GGTATCAACGCAGAGTACTTTTTTT7640.10904642894354849No Hit
CTGTAGGACGTGGAATATGGCAAGA7210.10290899904227549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGGC350.00211028116.3522873
AATCCCG702.5787813E-816.24258819
GATCGAG456.7487994E-414.7797817
TGCGCCA500.001521782613.27235812
TAGGACC2100.013.171734
ATTAACC603.9487146E-412.7184473
GGACGTG3150.011.8065926
AGGACGT3400.011.7815735
TTGGACT657.74869E-411.7392614
GGCGAGA2350.011.69235919
TGGCGAG3750.011.62247418
GTAGGAC3800.011.547013
GGTCTAC752.0030193E-411.4408471
TAAGGGA855.1003066E-511.2213524
GTCCTAA1701.0913936E-1111.21651651
CTTGCAC600.00569454711.1286413
ATGGCGA4100.011.09249917
GACTGGT700.00148793910.8586157
TGTAGGA4400.010.84036352
TAGGACG3350.010.8193934
Produced by FastQC (version 0.11.5)