FastQCFastQC Report
Thu 26 May 2016
SRR1926610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722126
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCG250.005175614719.507618
AACGCTT250.007246644718.40337611
ATCTTAC654.5318004E-516.4029451
CCCTAGG550.00461282714.5389751
GTAGGAC2800.014.4535943
TGTAGGA2900.014.1324932
GTATAGC955.1330862E-614.0288351
GTCCTAA1253.167588E-813.8604891
GTGCAAC400.00651604213.80253211
TAGGACG2000.013.3915734
ATACCTA704.6170826E-513.3854665
GCCTTAC600.00820975113.3273941
GTGCTAC700.001473256913.3273921
TAGGACC1451.0913936E-1113.2992194
CCTTACA853.5957986E-412.8577972
TTAGGCA756.7415756E-512.8559114
TGGACTG653.7056868E-412.81343655
GGACTGC653.726553E-412.804926
TTAGGTG600.002388912.4988024
TAGACTG758.813722E-512.4931025
Produced by FastQC (version 0.11.5)