FastQCFastQC Report
Thu 26 May 2016
SRR1926610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722126
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13510.18708646413506783No Hit
GGTATCAACGCAGAGTACTTTTTTT7930.10981463068771931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC551.8834163E-413.8814473
TAGGACC1950.013.7015694
ACTAGAC706.9153593E-613.6335643
GTAGGAC2750.012.8403393
GGCGAGA2150.012.77724919
GTCCTAA1800.012.7219971
TATGGCG3050.012.42332816
GTCCTAG853.7477857E-612.347821
GTAATAC550.002962554612.1462663
ACCTAAT550.002967203512.1437251
GTCCTAC3250.012.0369671
ATGGCGA3150.012.02893617
AGGACCT2650.011.8826945
TGGCGAG3050.011.80216218
ATTACTC751.9848143E-411.4521943
CTAATAC751.9848143E-411.4521943
TGCGTTG2350.011.29376412
TTGGACT855.078984E-511.2260744
TGTAGGA3500.011.1826432
CCTAGCC600.005674157811.1340773
Produced by FastQC (version 0.11.5)