##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926610_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 722126 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.386233150447428 31.0 28.0 31.0 22.0 34.0 2 28.857602412875316 31.0 28.0 33.0 23.0 34.0 3 29.224294652179815 31.0 29.0 33.0 25.0 34.0 4 33.01780852648984 35.0 33.0 37.0 27.0 37.0 5 32.68787164566849 35.0 33.0 37.0 26.0 37.0 6 32.73143606517422 35.0 33.0 37.0 26.0 37.0 7 32.55998953091289 35.0 33.0 37.0 25.0 37.0 8 32.53678859368033 35.0 33.0 37.0 25.0 37.0 9 33.778911159548336 37.0 34.0 39.0 23.0 39.0 10 33.71696352160149 37.0 33.0 39.0 23.0 39.0 11 33.92954415157465 37.0 34.0 39.0 25.0 39.0 12 33.736154078374135 37.0 34.0 39.0 23.0 39.0 13 33.87192678286061 37.0 34.0 39.0 25.0 39.0 14 34.538350647947865 38.0 34.0 40.0 22.0 41.0 15 34.64411473897907 38.0 34.0 40.0 24.0 41.0 16 34.61389840554141 38.0 34.0 40.0 24.0 41.0 17 34.53366586994513 38.0 34.0 40.0 23.0 41.0 18 34.50267266377336 38.0 34.0 40.0 23.0 41.0 19 34.540823900538136 39.0 34.0 40.0 22.0 41.0 20 34.53259957403556 39.0 34.0 40.0 22.0 41.0 21 34.45368952232713 39.0 34.0 40.0 19.0 41.0 22 34.38591049207479 39.0 34.0 40.0 19.0 41.0 23 34.03003077025339 39.0 34.0 40.0 17.0 41.0 24 34.104967831098726 39.0 34.0 40.0 18.0 41.0 25 33.902734425848124 39.0 34.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5229233090547893 1101 2 0.5335785851496837 1101 3 0.4109951196651025 1101 4 0.3928447858806621 1101 5 0.3690423719325082 1101 6 -0.17302371105645875 1101 7 0.057092582652195745 1101 8 0.065572381350961 1101 9 0.5625565185431114 1101 10 0.5104108611460703 1101 11 0.1717801408841808 1101 12 0.4230309535410015 1101 13 0.4321691217159511 1101 14 0.46425968421389285 1101 15 0.41741289601362297 1101 16 0.3367139467841085 1101 17 0.23752460889295435 1101 18 0.28527297809898755 1101 19 0.051052830225728485 1101 20 0.20679454754689175 1101 21 0.4554551939217575 1101 22 0.40942626521957237 1101 23 0.7891503035193068 1101 24 0.526730322121125 1101 25 0.7565828528978216 1102 1 0.5557901054839114 1102 2 0.5208572526423012 1102 3 0.5359280635014407 1102 4 0.3700365712254694 1102 5 0.577055780020757 1102 6 -0.03579456921812607 1102 7 0.3099576810588829 1102 8 0.7213379536316893 1102 9 0.522544192158044 1102 10 0.5985162140045617 1102 11 0.5354663589165511 1102 12 0.5138103772825033 1102 13 0.553879596313223 1102 14 0.9119521031726308 1102 15 0.7676859946215586 1102 16 0.4628380219121695 1102 17 0.8958152034209235 1102 18 0.7556893394884767 1102 19 0.7489966508390893 1102 20 0.6273406189833253 1102 21 0.8811701714229585 1102 22 0.7736250344677558 1102 23 1.054582025722837 1102 24 0.8773090647669548 1102 25 0.9128600330485668 1103 1 -0.11889274836889285 1103 2 -0.13011069302897837 1103 3 -0.12848745297548803 1103 4 -0.38812916455039925 1103 5 -0.33064918873512283 1103 6 -0.6299221421928465 1103 7 -0.26595101402467236 1103 8 -0.12419648188485155 1103 9 0.051889421329434526 1103 10 0.11678261995800909 1103 11 -0.05909311336246503 1103 12 -0.1635244786500536 1103 13 -0.43482850163924525 1103 14 -0.24007900378465052 1103 15 -0.14752777001564965 1103 16 -0.06966830187195683 1103 17 -0.17381883049944236 1103 18 -0.13861909530717043 1103 19 -0.18443216392586947 1103 20 -0.30267064102068986 1103 21 -0.1795260349445229 1103 22 -0.2606264304492001 1103 23 -0.050581308131498304 1103 24 -0.07537977953557373 1103 25 -0.04150218182292775 1104 1 0.5595748093121848 1104 2 0.7166274370502244 1104 3 0.6483958947727366 1104 4 0.42710240701684654 1104 5 0.6365333692703672 1104 6 0.13218935660493258 1104 7 0.3984653341600861 1104 8 0.5470867287906884 1104 9 0.9615115328660266 1104 10 0.9789206387916494 1104 11 0.6075456264255834 1104 12 0.761103815645491 1104 13 0.7186242369481661 1104 14 0.9264013087108225 1104 15 0.9454504678067082 1104 16 0.9233161722533723 1104 17 0.7247643948534588 1104 18 0.6198199139647613 1104 19 0.6993823621245525 1104 20 0.8522744007719893 1104 21 1.0478306733961844 1104 22 0.9242257786193946 1104 23 0.9679814571082304 1104 24 0.7470355091076115 1104 25 0.6794446779465488 1105 1 0.5832677339110113 1105 2 0.6031145164220746 1105 3 0.5522565191492532 1105 4 0.22524440034513304 1105 5 0.4951478975320214 1105 6 0.06699551654473623 1105 7 0.14199153939500775 1105 8 0.38918618686697215 1105 9 0.6007615408318898 1105 10 0.4541590631686532 1105 11 0.4065123928493577 1105 12 0.5294835102956199 1105 13 0.41434117678262083 1105 14 0.7762717715396619 1105 15 0.862566286538005 1105 16 0.8277019307627498 1105 17 0.7113933891204951 1105 18 0.607032254433058 1105 19 0.5896930552525887 1105 20 0.8749961029663993 1105 21 0.6703027377309354 1105 22 0.39585015268647084 1105 23 1.0247431258705362 1105 24 0.836335885831609 1105 25 0.844837419453107 1106 1 0.5868823011185498 1106 2 0.7939770434178506 1106 3 0.539109815022961 1106 4 0.5489094233751572 1106 5 0.7063313159139923 1106 6 -0.5397309708508615 1106 7 -0.2730959938121842 1106 8 -0.17557500801864023 1106 9 0.1372607859057524 1106 10 0.0212638736726376 1106 11 -0.3039208774141997 1106 12 0.1271246050340693 1106 13 -0.09119654085588991 1106 14 0.15485289265274815 1106 15 -0.011071979616460226 1106 16 -0.007392100542794822 1106 17 0.09937764078802047 1106 18 0.11286859791488979 1106 19 -0.015858040823708563 1106 20 -0.04627656870762564 1106 21 -0.015088963815230727 1106 22 -0.1525989041336686 1106 23 0.06213585946761668 1106 24 0.034838456402866314 1106 25 -0.0017716243708321144 1107 1 0.785869938014077 1107 2 0.8450354071465327 1107 3 0.7752356773890945 1107 4 0.7609297940819602 1107 5 0.44555203040267344 1107 6 0.3934702999309394 1107 7 0.5130983454325175 1107 8 0.7793606842732856 1107 9 0.9311197608022255 1107 10 0.43785891911426944 1107 11 0.4134081982411075 1107 12 0.6448476556557168 1107 13 0.38703293587827403 1107 14 0.7829954922586566 1107 15 0.6123232391136 1107 16 0.5866325930581127 1107 17 0.5921351627635048 1107 18 0.9011533452724763 1107 19 0.7392346358769188 1107 20 0.5170156319156547 1107 21 0.8346240854555163 1107 22 0.6109276085298987 1107 23 1.0239110876074164 1107 24 0.9565698500238042 1107 25 0.9156316345695927 1108 1 1.1654892399804062 1108 2 0.9712976366999406 1108 3 0.8611833641379754 1108 4 0.7055985379180925 1108 5 -0.496995905955643 1108 6 0.46628294448186125 1108 7 0.9834567880790743 1108 8 0.8901279278344987 1108 9 1.3029261127515355 1108 10 1.4345651218215707 1108 11 1.0205543594050184 1108 12 1.0590928489284863 1108 13 1.1963854350273309 1108 14 1.445584622902139 1108 15 1.1440716397803854 1108 16 1.2144369900243959 1108 17 1.270610276004156 1108 18 1.3929258341229627 1108 19 1.2811332385877137 1108 20 1.2714278715058072 1108 21 1.279698662821346 1108 22 1.530075096289437 1108 23 1.6460826897334613 1108 24 1.5788950579589383 1108 25 1.429835808109246 1109 1 0.5986314777945019 1109 2 -0.46683400054181945 1109 3 0.48942557057817737 1109 4 0.8619508001657934 1109 5 -0.7059992620112823 1109 6 0.5607031454649061 1109 7 0.7888302799931708 1109 8 0.8794113671735531 1109 9 1.3222410210542037 1109 10 -0.7421953813015563 1109 11 0.5026816993744063 1109 12 1.2067250708654882 1109 13 1.1304945591253315 1109 14 1.4886277002055053 1109 15 1.5150403324398454 1109 16 1.510174894438606 1109 17 1.5687192860152734 1109 18 1.3948405922112457 1109 19 1.7058178359085687 1109 20 1.6432122853369364 1109 21 1.5752369152005343 1109 22 1.4681544437124998 1109 23 1.6297055185175822 1109 24 1.5861890123051765 1109 25 1.8728921955482818 1110 1 0.5678514673880066 1110 2 -0.5687305947983461 1110 3 0.22270210351515374 1110 4 0.43087725194453697 1110 5 0.7486008445412864 1110 6 0.7010768257367204 1110 7 0.8524280668507203 1110 8 0.991150599456077 1110 9 1.2219074742011387 1110 10 0.846393543450354 1110 11 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0.08986617616583459 1111 23 0.4592997132597674 1111 24 0.2872284672521772 1111 25 0.33553918164056284 1112 1 0.4686170030219614 1112 2 0.29430640475181136 1112 3 0.5245785896021644 1112 4 0.8783641583130191 1112 5 0.873120878600794 1112 6 0.6529798204028765 1112 7 0.8936689896705872 1112 8 0.6460832034018367 1112 9 0.5492623196480864 1112 10 1.0604824516182134 1112 11 0.7284106075629921 1112 12 0.823246989806762 1112 13 0.7385383970078792 1112 14 1.0661711245231231 1112 15 0.9016718460874884 1112 16 0.8064941665643346 1112 17 0.8929024952791309 1112 18 0.6749015930383777 1112 19 1.0281141551806954 1112 20 0.6950371405892142 1112 21 0.9287573866645573 1112 22 0.816298571090492 1112 23 1.3363070487078232 1112 24 0.7971329742572024 1112 25 0.7771777621322329 1113 1 -0.17949453990665276 1113 2 -0.08487868861011094 1113 3 -1.4197169522543511 1113 4 -1.2862560284108255 1113 5 -1.4013726664720174 1113 6 -0.7690975940525888 1113 7 -0.4246772981878948 1113 8 -0.5952646255984284 1113 9 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0.09582148743314178 2202 18 0.16879213336030574 2202 19 -0.09996711554646964 2202 20 0.14476869846351548 2202 21 0.06288997988626477 2202 22 0.17388016536372675 2202 23 0.20028878857678478 2202 24 0.27712620001146604 2202 25 0.2624892763278197 2203 1 0.27899522400405985 2203 2 0.07863734414767265 2203 3 0.05861160797153886 2203 4 -0.12431052513763063 2203 5 -0.054489596593150225 2203 6 0.09985475434499591 2203 7 0.2240799225135106 2203 8 0.13971588461181028 2203 9 0.4962171836217024 2203 10 0.4659549504027467 2203 11 0.2574503109895474 2203 12 0.23356417331026336 2203 13 0.41185577075806634 2203 14 0.7716322613527709 2203 15 0.6788863703650847 2203 16 0.4795710896412544 2203 17 0.7302666802834636 2203 18 0.5438685271073282 2203 19 0.6162941549693102 2203 20 0.4689824605207207 2203 21 0.6984274407791702 2203 22 0.6809122910473704 2203 23 0.9173660093267557 2203 24 0.7508799091716938 2203 25 0.7133638709533372 2204 1 0.3015102931831528 2204 2 0.3436927399613161 2204 3 0.08505993883252927 2204 4 -0.14410136953704722 2204 5 -0.038525639384239696 2204 6 -0.19589876298594078 2204 7 0.03025132943939468 2204 8 0.1623503007812488 2204 9 0.48000091130819555 2204 10 0.45492378591989535 2204 11 0.0827619914924611 2204 12 0.11279539707743425 2204 13 0.05143304890725631 2204 14 0.6706971387658953 2204 15 0.4505645512056091 2204 16 0.284449138421742 2204 17 0.0992726560651036 2204 18 0.14460354889788363 2204 19 0.24383554859190326 2204 20 0.08909580124405636 2204 21 0.17371580321522373 2204 22 0.23028400049193465 2204 23 0.4730955405071313 2204 24 0.30958468298644704 2204 25 0.2553691468686594 2205 1 0.5870980770271998 2205 2 0.573812695096958 2205 3 0.7125894615569202 2205 4 0.528593613516172 2205 5 0.4765437206976415 2205 6 0.29595776672259433 2205 7 0.2827660578401421 2205 8 0.374238743302314 2205 9 0.7669893266628947 2205 10 0.7238977742794432 2205 11 0.4137378404106826 2205 12 0.5117000345536482 2205 13 0.36929913406245873 2205 14 0.8964717020957238 2205 15 0.7046638819809274 2205 16 0.6697503569834211 2205 17 0.5158777435953752 2205 18 0.7130489889653902 2205 19 0.7643087461833531 2205 20 0.7263733268114052 2205 21 0.6905580291890843 2205 22 0.5413364180837235 2205 23 0.9693354826666933 2205 24 0.8634657629935347 2205 25 0.9053198120153212 2206 1 0.5449186775379218 2206 2 0.8237249029203113 2206 3 0.8498009862531326 2206 4 0.8076387527661879 2206 5 1.1484512030382632 2206 6 0.9497158224960174 2206 7 1.25277417020434 2206 8 1.2056887764002724 2206 9 1.4608105928035044 2206 10 1.4971321114821592 2206 11 1.269701162246399 2206 12 1.1635792765676172 2206 13 0.8547733525285537 2206 14 1.4592899104990522 2206 15 1.3061394023407615 2206 16 1.4065726594999646 2206 17 1.3805725633063446 2206 18 1.3960390864972965 2206 19 1.2532090008112604 2206 20 1.4363538595845 2206 21 1.567379181536353 2206 22 1.6105980216399445 2206 23 1.8937540659762675 2206 24 1.6056265640007936 2206 25 1.651736239369285 2207 1 -0.7316958966674214 2207 2 0.15301240085388912 2207 3 0.3225260894499016 2207 4 0.5943163326232721 2207 5 0.30729965014595706 2207 6 0.45411341562791563 2207 7 0.6185456569463099 2207 8 0.5580468583138298 2207 9 0.7921116862355788 2207 10 0.48232224088674513 2207 11 0.5076012555927107 2207 12 0.5016607178048105 2207 13 0.21515847131203714 2207 14 -5.042772781926722 2207 15 -1.8883161251653249 2207 16 -0.47925709721543797 2207 17 -0.546546466289783 2207 18 0.07608124770674607 2207 19 0.3479436971747276 2207 20 0.551683784721007 2207 21 0.48323885630338737 2207 22 0.3688006266359025 2207 23 0.7714983666930806 2207 24 0.38103919570795597 2207 25 0.5966799394877356 2208 1 0.3022708491758088 2208 2 0.6551290543244619 2208 3 0.516202521226095 2208 4 0.4104261241249887 2208 5 0.6517141834440991 2208 6 0.6336137235367758 2208 7 0.6045806705822727 2208 8 0.6556451692138054 2208 9 0.7601419492777168 2208 10 0.8290223376580954 2208 11 0.6495965049988897 2208 12 0.5301273316871047 2208 13 0.4832819867514715 2208 14 0.8138349421869435 2208 15 0.6172772591929032 2208 16 0.6662377563079218 2208 17 0.8794480793247175 2208 18 0.7905248124115971 2208 19 0.49405788737443146 2208 20 0.5676475394496094 2208 21 0.8494304635876304 2208 22 0.6621568331987575 2208 23 0.9305271341779076 2208 24 0.8168516352258592 2208 25 0.9029647991692684 2209 1 -0.5027224031858637 2209 2 -0.38402524167193874 2209 3 0.10582015505425701 2209 4 0.3156532946153163 2209 5 0.2509464521500533 2209 6 0.19830708086171 2209 7 0.4967054269175506 2209 8 0.5521813815394907 2209 9 0.7143179876642805 2209 10 0.516234813291625 2209 11 0.40265993279916046 2209 12 0.512484506675861 2209 13 0.22838320132636625 2209 14 0.5549025685177256 2209 15 0.38009627253823197 2209 16 0.46580590745502093 2209 17 0.6583714561428309 2209 18 0.5948492988413463 2209 19 0.8411624007947296 2209 20 0.5086251972693745 2209 21 0.5394439588642825 2209 22 0.8932469516572965 2209 23 0.820441664092435 2209 24 0.9372055113692142 2209 25 0.9536200698245381 2210 1 -0.14119994120219914 2210 2 -0.29233944510280097 2210 3 0.3768783516957406 2210 4 0.33834135894743866 2210 5 0.5979837519817082 2210 6 0.4570539679048551 2210 7 0.7559476045265257 2210 8 0.6878903009434723 2210 9 1.1879006357992878 2210 10 1.139701986378988 2210 11 0.7866068142772349 2210 12 0.7503220625003948 2210 13 0.7780242547927045 2210 14 1.0589017615836553 2210 15 0.9822791683185272 2210 16 1.0268219487477808 2210 17 0.8964545703518212 2210 18 0.6992456982458819 2210 19 1.025115542968166 2210 20 1.2152344046653383 2210 21 1.1286400855807628 2210 22 1.1390335036836774 2210 23 1.405704682860609 2210 24 1.1653014252220402 2210 25 1.1093298239879061 2211 1 -1.4533118729105787 2211 2 -0.34217602581680495 2211 3 0.22161952747439884 2211 4 0.4088069988872789 2211 5 0.7473548607962712 2211 6 0.3777031919783269 2211 7 0.5584392235117264 2211 8 0.4942216429981059 2211 9 0.735565294383214 2211 10 0.7021310943365364 2211 11 0.546206121950803 2211 12 0.4687472719207335 2211 13 0.38421108210599186 2211 14 0.6412604606149017 2211 15 0.38696665519037055 2211 16 0.4469712526404379 2211 17 0.407192351714464 2211 18 0.14094408253746593 2211 19 0.5227167663022598 2211 20 0.35834390818998685 2211 21 0.5507344570676622 2211 22 0.4492780864813781 2211 23 0.552847155911131 2211 24 0.44190642106304523 2211 25 0.611045936541359 2212 1 1.0870066035306962 2212 2 0.16369317202941147 2212 3 0.5014244482017887 2212 4 0.3975573120001954 2212 5 0.5122252879941769 2212 6 0.35980143472006176 2212 7 0.7931205124613498 2212 8 0.636729708137139 2212 9 0.8575273772211602 2212 10 0.4608807505439714 2212 11 0.544589123835614 2212 12 0.6539202654534648 2212 13 0.39637321792950075 2212 14 0.7413374845876248 2212 15 0.5160103235238438 2212 16 -0.16701881292738818 2212 17 0.4702783781798132 2212 18 0.5246743383768901 2212 19 0.23358090020125388 2212 20 0.5075307530440725 2212 21 0.46398611755869723 2212 22 0.6448547303873511 2212 23 0.7752825716348539 2212 24 0.8524413081583191 2212 25 0.18392599065304438 2213 1 0.5079526673576282 2213 2 0.3460255189709258 2213 3 -0.04790101412744008 2213 4 -1.0967960980972364 2213 5 -1.9339886785087614 2213 6 -0.517329037405986 2213 7 -0.07777623177463511 2213 8 -0.06392553785690325 2213 9 -0.41475283186706235 2213 10 -0.45772850409274213 2213 11 -0.3675247071223211 2213 12 -0.5965519612421915 2213 13 -0.5495589223394362 2213 14 -7.459178525826527 2213 15 -3.3403153333996904 2213 16 -2.2098228497526833 2213 17 -1.1863515839749468 2213 18 -5.118877544490761 2213 19 -2.782200530133988 2213 20 -4.889898756846687 2213 21 -1.860796809139643 2213 22 -1.5546950186530921 2213 23 -4.442174886024112 2213 24 -2.2837965129222866 2213 25 -1.6499055712011028 2214 1 0.40824505148402324 2214 2 0.3358441482371113 2214 3 -1.4339309931436688 2214 4 -1.9371728801746286 2214 5 -0.18660972090831507 2214 6 -0.1182517060475945 2214 7 -0.262957988239485 2214 8 -0.3456673449296659 2214 9 -4.99535326079998 2214 10 -1.2509535241507592 2214 11 -0.5480545472753846 2214 12 -0.870203059804183 2214 13 -0.869019438910442 2214 14 -2.252866423129497 2214 15 -1.9692189314988084 2214 16 -1.0112639409086412 2214 17 -1.163010998898109 2214 18 -0.945024708596172 2214 19 -3.9697333172721727 2214 20 -1.7189678335608747 2214 21 -3.8834276491075457 2214 22 -6.2222951544304195 2214 23 -6.3984522729277735 2214 24 -5.460094883594383 2214 25 -3.186862461900354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22646.0 3 15.0 4 451.0 5 2810.0 6 3619.0 7 2850.0 8 2132.0 9 1764.0 10 1590.0 11 1404.0 12 1429.0 13 1350.0 14 1408.0 15 1358.0 16 1423.0 17 1556.0 18 1690.0 19 2059.0 20 2577.0 21 3367.0 22 4258.0 23 5409.0 24 6584.0 25 7588.0 26 8682.0 27 9715.0 28 11373.0 29 13643.0 30 16742.0 31 21324.0 32 27132.0 33 35191.0 34 46016.0 35 60465.0 36 81886.0 37 119800.0 38 188820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.63102145365937 19.060740551823933 9.027225273125957 30.28101272139073 2 13.911905256596718 21.086086294064685 38.620957129995155 26.381051319343445 3 18.268640756392728 24.102538530901597 29.071837086347724 28.556983626357948 4 11.963307886560308 17.02717416148242 37.23845025412142 33.771067697835846 5 13.156826759929361 38.22334568371481 35.15689601440493 13.462931541950898 6 32.321455880104 37.97814511532206 17.019004852006837 12.681394152567115 7 28.673745096052834 31.715406616495024 21.01782837191036 18.593019915541785 8 25.666788327752187 37.58134061264452 19.321075632024716 17.43079542757858 9 25.932541169482544 15.79400964252704 19.463943221780898 38.80950596620952 10 14.050093830716923 30.205596604089774 33.985970403506705 21.7583391616866 11 34.97481689103199 23.17012204494877 23.044379603689737 18.810681460329505 12 22.503943310497444 25.353374629038 31.270763832053056 20.871918228411502 13 28.6366922116085 21.857127426515593 25.52781647524116 23.978363886634742 14 20.63391982395581 22.13453578461193 27.40075689023706 29.8307875011952 15 23.016939106736313 30.288485506483724 23.127170703875834 23.56740468290413 16 22.27430723850937 29.119524726149752 26.527121906634722 22.079046128706153 17 21.67485569945603 28.740181469705195 27.318225629549207 22.266737201289565 18 21.551436425450593 28.549864631874865 28.40805700002077 21.490641942653767 19 22.835911632048546 27.697866135266167 27.41231684062278 22.053905392062507 20 22.809889201394228 27.734548642125073 27.93354576310618 21.522016393374518 21 22.681807166349316 27.141145923489702 27.429600138480183 22.747446771680803 22 22.354686024322625 27.961186828891353 27.415160235194413 22.268966911591605 23 22.272844351262798 27.633681656663793 27.37970935820065 22.71376463387276 24 22.24919508395361 27.597576596849578 28.10787606023888 22.045352258957937 25 22.326574586706474 27.385248557730925 27.74100364756289 22.547173207999712 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 206.0 1 206.0 2 207.5 3 209.0 4 209.0 5 209.0 6 338.0 7 467.0 8 467.0 9 467.0 10 863.5 11 1260.0 12 1260.0 13 1260.0 14 2063.0 15 2866.0 16 2866.0 17 2866.0 18 5091.5 19 7317.0 20 7317.0 21 7317.0 22 11781.0 23 16245.0 24 16245.0 25 16245.0 26 23321.5 27 30398.0 28 30398.0 29 30398.0 30 39362.5 31 48327.0 32 48327.0 33 48327.0 34 57222.5 35 66118.0 36 66118.0 37 66118.0 38 73553.0 39 80988.0 40 80988.0 41 80988.0 42 86233.5 43 91479.0 44 91479.0 45 91479.0 46 95020.5 47 98562.0 48 98562.0 49 98562.0 50 95439.5 51 92317.0 52 92317.0 53 92317.0 54 82246.0 55 72175.0 56 72175.0 57 72175.0 58 61743.0 59 51311.0 60 51311.0 61 51311.0 62 41553.5 63 31796.0 64 31796.0 65 31796.0 66 24621.0 67 17446.0 68 17446.0 69 17446.0 70 12645.5 71 7845.0 72 7845.0 73 7845.0 74 5503.5 75 3162.0 76 3162.0 77 3162.0 78 2162.0 79 1162.0 80 1162.0 81 1162.0 82 754.5 83 347.0 84 347.0 85 347.0 86 226.0 87 105.0 88 105.0 89 105.0 90 60.5 91 16.0 92 16.0 93 16.0 94 9.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0919507122025796 2 0.02437247793321387 3 0.02395703796844318 4 0.031434957334315616 5 0.020910478226791446 6 0.3927292466965599 7 0.21228982199782306 8 0.21395158185690588 9 0.21713662158681452 10 0.011216879048808658 11 0.0020771998238534547 12 0.0023541598003672492 13 0.0 14 0.06771671425762263 15 0.0013847998825689699 16 0.002215679812110352 17 0.003046559741651734 18 0.002908079753394837 19 0.001246319894312073 20 6.923999412844849E-4 21 1.38479988256897E-4 22 0.0 23 0.0 24 1.38479988256897E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 722126.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.97346039743967 #Duplication Level Percentage of deduplicated Percentage of total 1 92.26744996342444 80.24819405366642 2 6.0319935814447705 10.492467097467943 3 0.922342078599321 2.4065784673785076 4 0.3120999566448906 1.0857765287718812 5 0.14044099590289622 0.6107319697668765 6 0.07837708359805971 0.40900357058295994 7 0.052204421105413686 0.31782794061080727 8 0.040242325583004404 0.2800011448315446 9 0.02775366602771078 0.21724491358303832 >10 0.11192472900093779 1.6677065865934408 >50 0.008144804268493953 0.48947143964637296 >100 0.005748868153238625 0.9606924367288667 >500 0.0011178354660286954 0.6266653544048246 >1k 1.5969078086124221E-4 0.18763849596651552 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1351 0.18708646413506783 No Hit GGTATCAACGCAGAGTACTTTTTTT 793 0.10981463068771931 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 1.38479988256897E-4 12 0.0 0.0 0.0 0.0 1.38479988256897E-4 13 0.0 0.0 0.0 0.0 1.38479988256897E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGTAC 55 1.8834163E-4 13.881447 3 TAGGACC 195 0.0 13.701569 4 ACTAGAC 70 6.9153593E-6 13.633564 3 GTAGGAC 275 0.0 12.840339 3 GGCGAGA 215 0.0 12.777249 19 GTCCTAA 180 0.0 12.721997 1 TATGGCG 305 0.0 12.423328 16 GTCCTAG 85 3.7477857E-6 12.34782 1 GTAATAC 55 0.0029625546 12.146266 3 ACCTAAT 55 0.0029672035 12.143725 1 GTCCTAC 325 0.0 12.036967 1 ATGGCGA 315 0.0 12.028936 17 AGGACCT 265 0.0 11.882694 5 TGGCGAG 305 0.0 11.802162 18 ATTACTC 75 1.9848143E-4 11.452194 3 CTAATAC 75 1.9848143E-4 11.452194 3 TGCGTTG 235 0.0 11.293764 12 TTGGACT 85 5.078984E-5 11.226074 4 TGTAGGA 350 0.0 11.182643 2 CCTAGCC 60 0.0056741578 11.134077 3 >>END_MODULE