FastQCFastQC Report
Thu 26 May 2016
SRR1926609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811261
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC2650.016.5456471
TTGTGCG550.00470882814.4945341
GGGTAAG550.00470882814.4945341
GCCCTAT658.6179434E-414.3087061
TCCTATA600.0071095813.62097552
TCCTAGG802.2088E-413.62097552
TCCTACA3100.013.6209752
TAAGGCT952.485358E-713.4988564
GAATAAT802.7263857E-413.2866551
GTATAGA904.947403E-513.2866551
CTCTAGA904.947403E-513.2866551
CTTAGAC1151.0348231E-813.2694373
GTCTAAG958.211592E-512.58735751
TCTAGAA1305.8819023E-712.5732082
GTACTGG854.5492363E-412.5050871
GTGTTAG750.002533564312.4008791
TCTTAGA1101.8595229E-512.3827062
CCTACAC801.0493424E-412.2623813
GTAAGAC902.3371045E-512.1109933
TTAGACA1151.9140316E-712.0804994
Produced by FastQC (version 0.11.5)