##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926609_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 811261 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.811431832665445 27.0 2.0 30.0 2.0 31.0 2 16.013615840031754 16.0 2.0 30.0 2.0 31.0 3 19.34150661747576 22.0 12.0 30.0 2.0 31.0 4 21.772764868519502 28.0 2.0 35.0 2.0 35.0 5 21.89124461794663 28.0 2.0 35.0 2.0 35.0 6 22.640883759973672 30.0 2.0 35.0 2.0 36.0 7 22.593421845743848 30.0 2.0 35.0 2.0 37.0 8 22.934514293180616 30.0 2.0 35.0 2.0 37.0 9 25.578133301120108 31.0 17.0 37.0 2.0 39.0 10 26.321522913094554 31.0 19.0 37.0 2.0 39.0 11 23.524575937953383 30.0 7.0 37.0 2.0 39.0 12 24.66260796463777 31.0 10.0 37.0 2.0 39.0 13 21.399073787597334 27.0 2.0 35.0 2.0 39.0 14 24.59793703875818 30.0 10.0 37.0 2.0 40.0 15 25.70168540087592 31.0 11.0 38.0 2.0 40.0 16 26.02680271823741 31.0 10.0 38.0 2.0 40.0 17 25.32381194214932 31.0 8.0 38.0 2.0 40.0 18 25.357839215739446 31.0 10.0 38.0 2.0 40.0 19 25.77931393226101 31.0 8.0 38.0 2.0 40.0 20 26.043248720202254 31.0 2.0 39.0 2.0 40.0 21 23.898872249498005 30.0 2.0 37.0 2.0 40.0 22 24.795453004643388 31.0 2.0 38.0 2.0 40.0 23 24.75591332505815 31.0 2.0 38.0 2.0 40.0 24 23.887683495200683 31.0 2.0 38.0 2.0 40.0 25 23.66161938019947 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.310849759896822 1101 2 9.577708923652125 1101 3 6.273394286419705 1101 4 8.068662547317157 1101 5 7.3598773365987 1101 6 6.803453891498467 1101 7 6.374508125256394 1101 8 5.082366480675471 1101 9 5.145824934410758 1101 10 4.933890782231682 1101 11 7.714345512381826 1101 12 5.399713922140737 1101 13 9.517940144811014 1101 14 6.993622503600847 1101 15 6.121128008644931 1101 16 5.60795480261838 1101 17 2.196340834834576 1101 18 4.284328199736002 1101 19 4.878096925416418 1101 20 3.097073417987776 1101 21 0.4190635995910412 1101 22 3.80984705023646 1101 23 4.996397103417205 1101 24 6.3151973550980145 1101 25 6.712370973634098 1102 1 5.131961074751903 1102 2 9.886402308297185 1102 3 6.162345198147499 1102 4 7.789189699111439 1102 5 7.172707014844637 1102 6 6.150877787173087 1102 7 6.116490945670908 1102 8 6.338202753081685 1102 9 5.341544088434052 1102 10 4.717687580170324 1102 11 7.405468758166112 1102 12 4.597783064375989 1102 13 9.202873595945402 1102 14 6.719498331443013 1102 15 4.844194709921609 1102 16 5.278269174268019 1102 17 5.973731128630732 1102 18 5.842486121420524 1102 19 4.606251775065278 1102 20 4.918905827725894 1102 21 3.7704516119839653 1102 22 5.063522419416561 1102 23 5.490616675380238 1102 24 6.764948139181534 1102 25 6.512117590227376 1103 1 4.320192320450456 1103 2 8.366524513210727 1103 3 5.263806178555328 1103 4 7.239647285154966 1103 5 6.305577563031548 1103 6 5.895112144534725 1103 7 5.707251197489455 1103 8 5.474266850221724 1103 9 4.612225632333651 1103 10 3.889426134182827 1103 11 4.498811826819615 1103 12 3.644206016595586 1103 13 7.430309651853957 1103 14 5.884644098040987 1103 15 4.946701197427192 1103 16 4.494029182378618 1103 17 2.4185356651079637 1103 18 1.5039533602484632 1103 19 3.4532299914740356 1103 20 3.891775771517093 1103 21 6.0079708405410805 1103 22 1.6998541078837164 1103 23 3.7901883342904945 1103 24 5.244101361293058 1103 25 1.4635173511986217 1104 1 5.686312539029689 1104 2 10.069008297789438 1104 3 6.559704721876432 1104 4 7.662548568456565 1104 5 7.072717921241427 1104 6 6.787515664713055 1104 7 6.6951979923314795 1104 8 7.0042305493501935 1104 9 5.899600436897192 1104 10 5.025273825323559 1104 11 8.045615956914506 1104 12 5.100797836902402 1104 13 9.026901704362508 1104 14 6.972213178057707 1104 15 6.185139485945591 1104 16 5.736316214685619 1104 17 5.988896661408216 1104 18 3.765635042627448 1104 19 4.562964662289371 1104 20 3.9197618858532266 1104 21 2.884720093625493 1104 22 5.1008490923262 1104 23 5.247414839344749 1104 24 6.43219444322931 1104 25 5.291486824410281 1105 1 3.1797581277774576 1105 2 8.761512616731242 1105 3 5.810514755167503 1105 4 5.401842041478936 1105 5 1.7626253142317658 1105 6 3.987426134717726 1105 7 5.460068683584602 1105 8 2.4845181945815646 1105 9 3.461124967745185 1105 10 3.1593695538345052 1105 11 6.555073141928336 1105 12 3.8443407408624815 1105 13 4.9522210836212075 1105 14 3.8501548779042274 1105 15 3.984752634806352 1105 16 4.050885321461724 1105 17 4.755354980839375 1105 18 2.2913753796902725 1105 19 2.0778307194847656 1105 20 1.7974255422359917 1105 21 2.1739344169418473 1105 22 3.671797442972373 1105 23 1.3255126142555902 1105 24 4.1338158073476166 1105 25 5.036228095699144 1106 1 6.213557249360409 1106 2 10.700339899655575 1106 3 7.290037955317427 1106 4 8.796965733659995 1106 5 8.283483355532617 1106 6 7.299884278540375 1106 7 7.082974037408825 1106 8 6.838575292608386 1106 9 5.844890420655435 1106 10 5.423685629638168 1106 11 7.737235359706315 1106 12 4.9949595921957695 1106 13 4.437258504995899 1106 14 5.563555009639224 1106 15 5.5635543240275105 1106 16 5.5807829190893905 1106 17 5.968982938790738 1106 18 6.088670678294193 1106 19 5.89301634732513 1106 20 5.280208127126521 1106 21 5.6108490620336156 1106 22 6.109346878046036 1106 23 6.3385800094762 1106 24 7.0653338189391945 1106 25 5.981922318007921 1107 1 3.786271017872437 1107 2 -13.355855090188705 1107 3 -5.219335991101152 1107 4 1.5232803047082442 1107 5 4.356248109347099 1107 6 4.4562185363459506 1107 7 4.542158235823411 1107 8 5.099850623346828 1107 9 3.383784397867302 1107 10 2.7665608699810456 1107 11 5.334584675171211 1107 12 2.7670001709845984 1107 13 4.287363553092035 1107 14 3.836586356873017 1107 15 3.5878733946435304 1107 16 3.282907031095373 1107 17 4.01654901069012 1107 18 -0.8158159364562678 1107 19 1.6958768524562728 1107 20 2.096611430974118 1107 21 -1.5458756237637168 1107 22 1.6480525311677603 1107 23 -1.1928048891365393 1107 24 2.1650234057193956 1107 25 3.007734201533605 1108 1 5.356927014328008 1108 2 10.691763957430343 1108 3 0.24406961537481564 1108 4 6.143250029368801 1108 5 7.539897579990171 1108 6 7.537992412789336 1108 7 7.516729719776556 1108 8 7.227881157134291 1108 9 5.903010161442747 1108 10 5.0134331874290545 1108 11 7.057292102782693 1108 12 2.6527633772168855 1108 13 3.443468003013116 1108 14 4.6229966061830545 1108 15 4.945396539586717 1108 16 4.5997209208303715 1108 17 0.2498869591666626 1108 18 3.599896780109326 1108 19 4.615445982686385 1108 20 4.892146741434804 1108 21 4.740081418483303 1108 22 1.5021733925380865 1108 23 4.356986044876145 1108 24 5.172271949641711 1108 25 5.5234930695312805 1109 1 3.5987010923802814 1109 2 -13.355855090188705 1109 3 -5.129083505522665 1109 4 2.2355760825971096 1109 5 2.57924893456709 1109 6 3.040283855682283 1109 7 3.830851333334259 1109 8 4.237225947682088 1109 9 3.732789968102253 1109 10 2.958381868655934 1109 11 0.9515933320463255 1109 12 1.61890492155392 1109 13 2.9847676807789583 1109 14 3.515030261750546 1109 15 3.624287218665369 1109 16 3.3567265300916205 1109 17 3.9351745829881217 1109 18 4.047187626272354 1109 19 2.7359466390892955 1109 20 1.7425017957372262 1109 21 0.9723674908069704 1109 22 3.0598055682153777 1109 23 3.615021013249457 1109 24 4.693434976255865 1109 25 5.085502259172049 1110 1 4.131704735800344 1110 2 9.861601644198053 1110 3 7.0139042309366175 1110 4 8.556678877205904 1110 5 9.096820845910536 1110 6 5.022640990053198 1110 7 7.605911396962103 1110 8 8.200435325528513 1110 9 6.472379489309333 1110 10 6.063745682630298 1110 11 4.122223538110774 1110 12 6.646877411092287 1110 13 11.042755483242228 1110 14 8.754258411130767 1110 15 7.979720336825704 1110 16 7.574457181022936 1110 17 8.097057610722995 1110 18 8.046893908531803 1110 19 7.258359942852842 1110 20 7.439228328019777 1110 21 5.398959506964747 1110 22 7.587830860943058 1110 23 7.871141232910727 1110 24 8.930265530821362 1110 25 9.286891973110773 1111 1 5.496248658478624 1111 2 9.66879279713524 1111 3 7.886850107853498 1111 4 8.731589590545383 1111 5 9.195349338158227 1111 6 8.505727394105808 1111 7 8.316715347830595 1111 8 8.476798470538526 1111 9 7.1352176923424295 1111 10 6.470561672625955 1111 11 8.121295168797829 1111 12 7.255359908787263 1111 13 8.575316235263763 1111 14 7.123073256561515 1111 15 6.708681008303785 1111 16 6.455915229539773 1111 17 7.724007875138685 1111 18 7.403422697518547 1111 19 6.519714450636943 1111 20 6.107103146532054 1111 21 8.603643744822289 1111 22 8.203706400107311 1111 23 8.016069114723802 1111 24 7.753296190323901 1111 25 8.161420634923633 1112 1 -2.2359227874632133 1112 2 7.549900305494749 1112 3 5.905362621786015 1112 4 0.22433454950779108 1112 5 3.874420166002995 1112 6 3.2176282959195674 1112 7 5.936418458581432 1112 8 6.023809312569128 1112 9 4.560402614826977 1112 10 3.4039338481249892 1112 11 -0.12874029578824775 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2204 7 4.607144940204883 2204 8 4.709003486094105 2204 9 3.1734680128966666 2204 10 2.6755995924526026 2204 11 4.164093383633777 2204 12 4.413983066221636 2204 13 2.1564517427334238 2204 14 3.698321499725509 2204 15 3.8740174163072005 2204 16 3.8945576699266233 2204 17 -1.117645290148026 2204 18 2.3446583968237604 2204 19 2.9461763270471337 2204 20 3.0124377653955534 2204 21 4.454008033589474 2204 22 4.018339576725243 2204 23 2.9234587595795283 2204 24 4.5288828791228894 2204 25 4.6226663732917395 2205 1 6.000508906823477 2205 2 10.13210086301157 2205 3 6.96688752436911 2205 4 7.7781779991802615 2205 5 7.730174323860467 2205 6 7.119219149870954 2205 7 7.5934351989400035 2205 8 7.208127047644659 2205 9 6.110986621125036 2205 10 5.499518275283883 2205 11 7.86727192402984 2205 12 6.925381137328504 2205 13 9.874587291747194 2205 14 7.24048938443682 2205 15 6.599184386452926 2205 16 6.240416455754595 2205 17 6.995066297247387 2205 18 6.607111035391618 2205 19 5.339218051734235 2205 20 5.453661505633669 2205 21 -0.7739393033635906 2205 22 4.018787388446157 2205 23 5.273823832985194 2205 24 6.980364519085182 2205 25 7.039302724432471 2206 1 3.7668407472630427 2206 2 8.25905599052265 2206 3 5.789979141976154 2206 4 1.6238729762219606 2206 5 5.2795683320119124 2206 6 5.687150680747681 2206 7 5.73289711696799 2206 8 5.54486748654978 2206 9 4.4650331101571155 2206 10 3.728740705785235 2206 11 3.4044477092113077 2206 12 4.659760594708533 2206 13 7.7397716407472785 2206 14 0.12117541258933784 2206 15 2.1587879426595755 2206 16 2.953323757313804 2206 17 3.829832425725378 2206 18 -1.0413336655573104 2206 19 0.9778877035607785 2206 20 2.2425147956332268 2206 21 3.0752396678295533 2206 22 -2.335071272769895 2206 23 -2.958483398715245 2206 24 0.8920863870445821 2206 25 2.425310540705798 2207 1 1.6437217322151625 2207 2 -13.355855090188705 2207 3 -5.810812337406297 2207 4 -4.041783984236641 2207 5 1.2516222698661217 2207 6 1.892414181496818 2207 7 3.086717714974636 2207 8 3.2194978037929545 2207 9 1.0837224463567132 2207 10 0.8372026952321789 2207 11 -1.3143165406746924 2207 12 -1.6018184555162058 2207 13 0.9448885712404085 2207 14 -4.704194270167488 2207 15 -1.161826349568944 2207 16 -0.0523599115025597 2207 17 1.138802525393153 2207 18 1.0918335548192104 2207 19 0.4811122491929858 2207 20 0.3445276938157562 2207 21 -2.714920582317017 2207 22 -3.4864620616435857 2207 23 -0.7423736990214174 2207 24 0.902444018469243 2207 25 1.5510841059458471 2208 1 4.1383741386322725 2208 2 5.79922512371504 2208 3 5.683649083525712 2208 4 7.485877080189127 2208 5 2.808917915397064 2208 6 5.3053884621181595 2208 7 5.852378524753092 2208 8 5.715741755919261 2208 9 4.222059599399746 2208 10 3.7695938335056702 2208 11 5.849230696401957 2208 12 5.399422950136966 2208 13 7.912962164313999 2208 14 2.7826913931887667 2208 15 4.190764686845622 2208 16 4.459417526745465 2208 17 5.2668582931548045 2208 18 -0.2486617342054913 2208 19 2.413980851179236 2208 20 3.5254864409509743 2208 21 6.039376591097806 2208 22 5.1483418599250435 2208 23 1.0651765938229936 2208 24 2.5633492896686327 2208 25 3.876109570677194 2209 1 2.7297364799946138 2209 2 4.633461149127534 2209 3 5.36015776003439 2209 4 0.054776862324203535 2209 5 4.716207249156984 2209 6 5.445629484712121 2209 7 6.016767512671493 2209 8 4.638373046197611 2209 9 4.500652466227908 2209 10 3.4462548631078747 2209 11 -0.2938707555230238 2209 12 3.2846344162307837 2209 13 2.500174788291332 2209 14 3.8037542083692273 2209 15 4.100539280443744 2209 16 3.6928338925147735 2209 17 1.1850808657891392 2209 18 3.422581238508073 2209 19 4.034136022804997 2209 20 4.069293668581729 2209 21 6.154797390342132 2209 22 5.0235932425293655 2209 23 0.9869470891229035 2209 24 -2.7580757449916966 2209 25 0.1828744289126405 2210 1 -2.5012627649244514 2210 2 -13.355855090188705 2210 3 -7.592722651643349 2210 4 -2.770122090080317 2210 5 -1.166855143370885 2210 6 -1.5895563349948567 2210 7 -1.7492763708556858 2210 8 -20.20137054354598 2210 9 -12.795826255083776 2210 10 -7.4068628597203165 2210 11 -9.660914645395422 2210 12 -4.82280440000536 2210 13 -5.282580812949794 2210 14 -4.293345075255669 2210 15 -5.874451043098915 2210 16 -5.358335531149084 2210 17 -4.994983728529352 2210 18 -5.250038828878143 2210 19 -10.426921788475532 2210 20 -7.489601057907652 2210 21 -5.509067862609978 2210 22 -9.844185106318024 2210 23 -8.150401498114327 2210 24 -10.71800728009027 2210 25 -8.401875474322786 2211 1 0.016937882580634778 2211 2 4.84532834176396 2211 3 1.0441923863767073 2211 4 1.8352940659183297 2211 5 1.1424345119227084 2211 6 0.2563899493187414 2211 7 -19.862862116958137 2211 8 -12.774595395616986 2211 9 -8.68612158593076 2211 10 -6.961234727074022 2211 11 -9.477110948378602 2211 12 -4.689587586837373 2211 13 -6.766672680479216 2211 14 -4.6758370692989715 2211 15 -5.047444502155422 2211 16 -11.351818748291715 2211 17 -7.334999125444696 2211 18 -5.639055842359777 2211 19 -8.323441229003027 2211 20 -6.578443565693966 2211 21 -4.311124152502487 2211 22 -5.767251596392395 2211 23 -7.76612106779141 2211 24 -21.248816485732437 2211 25 -21.033649787611576 2212 1 -1.0853938321852645 2212 2 -13.355855090188705 2212 3 -7.381439250642087 2212 4 -19.065593508046167 2212 5 -19.134932351982616 2212 6 -19.84568105659842 2212 7 -9.415183124039176 2212 8 -8.946453155662423 2212 9 -4.9757435115449375 2212 10 -5.45645875046333 2212 11 -5.766689228538194 2212 12 -2.8223785914509882 2212 13 -5.566072933739239 2212 14 -3.7358600442662677 2212 15 -3.7135660855892354 2212 16 -3.853317824171956 2212 17 -3.9908512243837038 2212 18 -3.0093974175067757 2212 19 -3.5235125747727913 2212 20 -3.6935750374419705 2212 21 -1.6187629217705002 2212 22 -2.784243644772509 2212 23 -2.875906262322111 2212 24 -1.9238754943870404 2212 25 -2.3286930606249534 2213 1 -18.179807679549544 2213 2 -13.355855090188705 2213 3 -8.284915062608526 2213 4 -5.778422734894086 2213 5 -19.134932351982616 2213 6 -10.19997162584226 2213 7 -19.862862116958137 2213 8 -10.288881163766881 2213 9 -8.13049425136741 2213 10 -7.17970398247714 2213 11 -6.624456144519627 2213 12 -5.0927368279628915 2213 13 -18.58102179290525 2213 14 -11.970157324888099 2213 15 -7.0569800780101914 2213 16 -6.139612909558199 2213 17 -4.783575261745494 2213 18 -4.893765187651439 2213 19 -5.6654896371010395 2213 20 -5.71992187530671 2213 21 -3.9925652850579247 2213 22 -4.667022476123737 2213 23 -4.551933206766741 2213 24 -3.634542908842036 2213 25 -4.502638742581841 2214 1 -18.179807679549544 2214 2 -13.355855090188705 2214 3 -11.876007267837485 2214 4 -19.065593508046167 2214 5 -19.134932351982616 2214 6 -19.84568105659842 2214 7 -15.745215058134608 2214 8 -14.554311720016567 2214 9 -13.750391527101524 2214 10 -13.647299302288141 2214 11 -14.442169321131136 2214 12 -12.324989691223603 2214 13 -18.58102179290525 2214 14 -16.313587207670356 2214 15 -23.051882371977907 2214 16 -17.788675749313096 2214 17 -22.73514705443878 2214 18 -17.28093358482353 2214 19 -14.65272161222043 2214 20 -13.508757882709936 2214 21 -11.015754030450367 2214 22 -12.03692814971816 2214 23 -11.92178316772693 2214 24 -10.815836736455678 2214 25 -10.580419315094701 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 158460.0 3 3.0 4 816.0 5 2948.0 6 3645.0 7 3526.0 8 3032.0 9 2883.0 10 2768.0 11 2629.0 12 2507.0 13 2801.0 14 4067.0 15 7951.0 16 11732.0 17 12655.0 18 13055.0 19 15194.0 20 18377.0 21 21355.0 22 22247.0 23 24127.0 24 25479.0 25 25408.0 26 21248.0 27 17356.0 28 18955.0 29 21579.0 30 21650.0 31 25003.0 32 31373.0 33 38646.0 34 46451.0 35 51930.0 36 49790.0 37 49825.0 38 29790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21865563230853 18.846644163126665 12.181276320600622 25.75342388396418 2 14.735071101853572 20.462118932964394 40.55095552996319 24.251854435218846 3 19.232481837356456 24.485847459833867 28.783428377991306 27.498242324818374 4 11.34081604280806 16.953841177789066 39.411899891334265 32.29344288806861 5 13.608338538546194 37.44283118255354 35.94599609361168 13.002834185288581 6 31.163423778502562 37.40207510419983 18.065224936820336 13.369276180477268 7 28.80861556961151 31.072193175955377 22.16575465451192 17.953436599921186 8 24.678834169032964 38.15280844378648 20.165789659532905 17.002567727647644 9 26.1812218668562 15.903863618415857 19.94442470188739 37.970489812840555 10 13.675737935928122 29.888091585506622 35.163327819947135 21.27284265861812 11 33.2220167235406 22.82515824845659 24.35645627653755 19.596368751465263 12 22.84888066017223 25.285726561828646 31.940180354720184 19.925212423278936 13 27.662473794549264 21.461972979268577 25.349988353133007 25.52556487304915 14 20.386979592755765 22.334886955825585 28.264921405715633 29.013212045703018 15 22.442529354461534 30.92311531625798 23.92542212279406 22.70893320648642 16 21.986823178419847 28.89983433838098 27.27505663699303 21.838285846206144 17 21.37525423814674 28.52900277857282 28.662359076713432 21.433383906567006 18 21.169294610995493 28.120566711038613 29.922942797682662 20.787195880283228 19 22.200330827969456 27.641376484228203 27.67885699436293 22.479435693439413 20 22.555296101305004 27.946024725481884 28.5827658372509 20.915913335962212 21 21.78652822034868 26.474387731099135 27.247171152503896 24.49191289604829 22 22.112639404274272 28.15134356069231 28.291342109411556 21.44467492562186 23 21.880390603599533 27.851112608243938 28.22769909604436 22.040797692112164 24 21.618986179353286 27.9035327244621 28.62844383494207 21.849037261242547 25 22.288211094192295 27.59530408215142 28.327529657973905 21.788955165682385 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 781.0 1 781.0 2 1665.5 3 2550.0 4 2550.0 5 2550.0 6 3678.0 7 4806.0 8 4806.0 9 4806.0 10 5736.0 11 6666.0 12 6666.0 13 6666.0 14 8298.5 15 9931.0 16 9931.0 17 9931.0 18 14055.0 19 18179.0 20 18179.0 21 18179.0 22 25135.0 23 32091.0 24 32091.0 25 32091.0 26 41452.0 27 50813.0 28 50813.0 29 50813.0 30 60690.0 31 70567.0 32 70567.0 33 70567.0 34 79340.5 35 88114.0 36 88114.0 37 88114.0 38 93372.5 39 98631.0 40 98631.0 41 98631.0 42 99636.0 43 100641.0 44 100641.0 45 100641.0 46 98833.5 47 97026.0 48 97026.0 49 97026.0 50 90007.0 51 82988.0 52 82988.0 53 82988.0 54 72190.0 55 61392.0 56 61392.0 57 61392.0 58 51096.0 59 40800.0 60 40800.0 61 40800.0 62 32315.5 63 23831.0 64 23831.0 65 23831.0 66 18179.5 67 12528.0 68 12528.0 69 12528.0 70 9015.5 71 5503.0 72 5503.0 73 5503.0 74 3879.5 75 2256.0 76 2256.0 77 2256.0 78 1519.0 79 782.0 80 782.0 81 782.0 82 532.0 83 282.0 84 282.0 85 282.0 86 176.5 87 71.0 88 71.0 89 71.0 90 49.0 91 27.0 92 27.0 93 27.0 94 16.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.687408861020067 2 35.67335296532189 3 5.325166623318513 4 13.108851528669566 5 12.97215076282479 6 14.057498141781743 7 8.971711939807287 8 7.49351934827386 9 0.10218659592905366 10 1.3294118662181469 11 5.3596807932342365 12 5.3273853913845235 13 15.331810601027291 14 1.4593330629723356 15 2.6943240214924664 16 2.8236288937838747 17 2.6683151291631177 18 1.3813063859842887 19 1.3368077597715162 20 0.28326272309404743 21 4.103981332764671 22 1.4751109692195237 23 0.10144700657371672 24 8.8578151790854 25 0.10539148313551373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 811261.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.1000743424621 #Duplication Level Percentage of deduplicated Percentage of total 1 97.39481162383565 92.62253825996861 2 2.027959401429609 3.857181796789015 3 0.2924413022367431 0.8343356875056208 4 0.10205975087766547 0.3882355958335659 5 0.05219094646339613 0.24816814443362153 6 0.02873901082082408 0.16398492393535155 7 0.019690450791228598 0.1310794333857702 8 0.016649989468640283 0.12667321890151215 9 0.009124347878912831 0.07809535454499869 >10 0.050012283574155235 0.81915898993534 >50 0.004082585930626689 0.2642531343737138 >100 0.0022383066925746667 0.4662954603928873 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAC 265 0.0 16.545647 1 TTGTGCG 55 0.004708828 14.494534 1 GGGTAAG 55 0.004708828 14.494534 1 GCCCTAT 65 8.6179434E-4 14.308706 1 TCCTATA 60 0.00710958 13.6209755 2 TCCTAGG 80 2.2088E-4 13.6209755 2 TCCTACA 310 0.0 13.620975 2 TAAGGCT 95 2.485358E-7 13.498856 4 GAATAAT 80 2.7263857E-4 13.286655 1 GTATAGA 90 4.947403E-5 13.286655 1 CTCTAGA 90 4.947403E-5 13.286655 1 CTTAGAC 115 1.0348231E-8 13.269437 3 GTCTAAG 95 8.211592E-5 12.5873575 1 TCTAGAA 130 5.8819023E-7 12.573208 2 GTACTGG 85 4.5492363E-4 12.505087 1 GTGTTAG 75 0.0025335643 12.400879 1 TCTTAGA 110 1.8595229E-5 12.382706 2 CCTACAC 80 1.0493424E-4 12.262381 3 GTAAGAC 90 2.3371045E-5 12.110993 3 TTAGACA 115 1.9140316E-7 12.080499 4 >>END_MODULE