FastQCFastQC Report
Thu 26 May 2016
SRR1926609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811261
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14050.17318717404139974No Hit
GGTATCAACGCAGAGTACTTTTTTT8180.10083068211093593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG350.00220999716.24011819
GTAGGAC4600.012.4448293
CTGTAGG5000.012.2041911
TGTAGGA5200.012.1105282
TAGGACG3300.011.8525434
AGGACGT3100.011.6940125
TAGGACC2300.011.6137314
GGCGAGG1552.7284841E-1111.61255719
GACGTGG2250.011.3982947
TAGACAG1701.0913936E-1111.2233545
GGACGTG3400.011.2226576
GCATTAC600.00571507111.1236111
GTGTTAA951.3043184E-511.0399751
ATAGCCC803.619343E-410.7336653
TAGACAC803.6235E-410.7323325
GTCATAG803.6422585E-410.726341
TACACTG3350.010.5365515
AGGACCT4000.010.4938365
GTCCTAC4850.010.419171
TCCTACA5350.010.3441942
Produced by FastQC (version 0.11.5)