FastQCFastQC Report
Thu 26 May 2016
SRR1926608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794708
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACC400.002422505816.0171134
TCCTAGA1301.546141E-1015.6794552
GTCTAGG550.00475574514.4728031
GTATAGG751.5975087E-414.1511841
TAGGACC1450.013.99196154
GTCCTAC3950.013.7705361
GTCCTAA2050.013.5903161
GACAGTC602.2903954E-413.5640837
GTCTAGC600.00846284113.2667361
CTATACT1055.902075E-813.2204754
GTAGGAC3400.013.1411623
CCTAGAT600.002102088612.7138053
GTACTTG1051.5115766E-512.6349861
CTAAGAC952.9375751E-612.6182143
GTAGAAC1655.456968E-1212.5486923
AAAGTGT2650.012.5333696
GTGTAGC854.6067554E-412.486341
GTCTTAC854.6067554E-412.486341
CTAAAGT2850.012.3640894
AGGACGT2400.012.1142645
Produced by FastQC (version 0.11.5)