FastQCFastQC Report
Thu 26 May 2016
SRR1926608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794708
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13220.16635040794857986No Hit
GTCCTACAGTGGACATTTCTAAATT11100.1396739431338303No Hit
CTGTAGGACGTGGAATATGGCAAGA10550.1327531621677396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2950.015.5216964
TCCAACG801.330609E-714.210031518
GTCTAGG905.149559E-712.7151391
GTAGGAC6800.012.4853593
GTTCTAG853.7718164E-612.3411651
ACCCTAC701.0514226E-412.2610261
TGTAGGA6700.012.2484522
AGGACCT5350.012.1248145
CCAGGAC2200.011.7074163
CCAACGA658.2069356E-411.65951419
CTACACT1406.330083E-1011.5835274
CTGTAGG7300.011.4958781
TATGGCG6100.011.4929916
TGGCGAG5950.011.46355618
GTCTAAA751.9991542E-411.44362451
ATGGCGA6300.011.27851317
CTCTTAG1201.2060809E-711.1257471
GGACGTG5250.011.0838456
GTCTTAG951.3014527E-511.0420941
GGCGAGG1900.010.96914819
Produced by FastQC (version 0.11.5)