FastQCFastQC Report
Thu 26 May 2016
SRR1926607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630825
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC1500.016.7458611
GCACCGT752.048182E-816.5458456
GTAGGAC1850.016.4110013
TTTGGAC400.002185932116.2644753
GTATAGG500.002580002915.86451
CTAGCAC651.5640524E-515.0133613
TGTAGGA2000.014.8825382
TAGGACC951.638E-814.5743744
AGCACCG507.8664324E-414.4822385
TTATACC450.005394206414.2006724
GTCCTAA751.644311E-414.1017771
GTCTATG905.1791907E-513.2204171
CCTACAG2500.013.01157953
TCCTACA2200.012.9145982
GGACTGT902.8807754E-612.6391886
CTAGGCT600.00249586612.4255884
GATTAAA750.002628513612.3390551
CTGTAGG2151.8189894E-1212.2980611
CTATAGT705.709965E-412.1720054
AGGACGT1701.8189894E-1212.1699485
Produced by FastQC (version 0.11.5)