##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926607_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 630825 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.705574446161773 27.0 2.0 30.0 2.0 31.0 2 16.01173067015416 16.0 2.0 30.0 2.0 31.0 3 19.285277216343676 22.0 12.0 30.0 2.0 31.0 4 21.692383783141125 28.0 2.0 35.0 2.0 35.0 5 21.824785003764912 28.0 2.0 35.0 2.0 35.0 6 22.54095826893354 30.0 2.0 35.0 2.0 35.0 7 22.502350097095075 30.0 2.0 35.0 2.0 36.0 8 22.783653152617603 30.0 2.0 35.0 2.0 37.0 9 25.37099195497959 31.0 17.0 37.0 2.0 39.0 10 26.08982522886696 31.0 17.0 37.0 2.0 39.0 11 23.345056077359015 30.0 7.0 37.0 2.0 39.0 12 24.41383902033052 30.0 8.0 37.0 2.0 39.0 13 21.234816311972416 27.0 2.0 35.0 2.0 39.0 14 24.372756311179803 30.0 10.0 37.0 2.0 40.0 15 25.436677366940117 31.0 10.0 38.0 2.0 40.0 16 25.76610153370586 31.0 8.0 38.0 2.0 40.0 17 25.030691554710103 31.0 7.0 38.0 2.0 40.0 18 25.048293900844133 30.0 8.0 38.0 2.0 40.0 19 25.44549756271549 31.0 2.0 38.0 2.0 40.0 20 25.704729520865534 31.0 2.0 38.0 2.0 40.0 21 23.57428763920263 30.0 2.0 37.0 2.0 40.0 22 24.464312606507352 30.0 2.0 37.0 2.0 40.0 23 24.42619268418341 31.0 2.0 38.0 2.0 40.0 24 23.60690999881108 30.0 2.0 38.0 2.0 40.0 25 23.36651052193556 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.770445484305988 1101 2 10.042666690793904 1101 3 6.762210478874611 1101 4 8.50084883764329 1101 5 7.843090178773 1101 6 7.462971213947984 1101 7 7.232901118559042 1101 8 5.831277735704461 1101 9 5.812637429426651 1101 10 5.335764304137015 1101 11 8.350731516348826 1101 12 5.598308067277831 1101 13 10.234059794313488 1101 14 7.961996916056393 1101 15 7.033609276623245 1101 16 6.633146424891994 1101 17 2.528564092421963 1101 18 5.012666349683343 1101 19 5.486646820365024 1101 20 3.3558514197866174 1101 21 0.32219474719400054 1101 22 4.1676766160170295 1101 23 5.5095862912775715 1101 24 7.051552954166095 1101 25 7.4213860060435195 1102 1 4.954953050713506 1102 2 9.46788392259161 1102 3 5.7971192947376835 1102 4 7.221804770783226 1102 5 6.813297850182494 1102 6 5.809638514702776 1102 7 5.579439337088456 1102 8 5.654431312603119 1102 9 4.886874806355053 1102 10 4.358163467140692 1102 11 6.925019091920429 1102 12 4.262056418837421 1102 13 8.801928737356523 1102 14 6.565073602221268 1102 15 4.401554008294514 1102 16 4.804572149394293 1102 17 5.516500565850272 1102 18 5.597641329622757 1102 19 4.48321066662281 1102 20 4.813484142357211 1102 21 3.658740793692285 1102 22 4.608989118045734 1102 23 5.241529310910902 1102 24 6.617919712247133 1102 25 6.221084361264214 1103 1 4.308463888430982 1103 2 8.21154948963931 1103 3 5.4523689872860785 1103 4 7.15228699903437 1103 5 6.331598873006367 1103 6 5.9683532856309895 1103 7 5.908432313940661 1103 8 5.764290144465257 1103 9 4.944590466122559 1103 10 3.945931891570364 1103 11 4.903672225292045 1103 12 3.6355053090281864 1103 13 7.880994363453524 1103 14 6.086637060464806 1103 15 5.026347587573632 1103 16 4.913604135984986 1103 17 2.8631711381403377 1103 18 1.6215454496679804 1103 19 3.57046957768231 1103 20 4.038215572629046 1103 21 6.232633406097136 1103 22 2.1551990747506586 1103 23 4.241778836850365 1103 24 5.395148842928204 1103 25 0.9254635415017631 1104 1 5.544231663614166 1104 2 9.818745659280122 1104 3 6.499618239362611 1104 4 7.382611811511527 1104 5 7.008530002107559 1104 6 6.825111230962893 1104 7 6.8179862232731345 1104 8 6.892628701391061 1104 9 5.90659502489417 1104 10 4.767529691467544 1104 11 7.840797102186631 1104 12 5.080386601340834 1104 13 9.199701895956451 1104 14 6.886699155146033 1104 15 6.269536528836088 1104 16 5.734823328661218 1104 17 5.833971153005898 1104 18 3.23750223424744 1104 19 4.236487682695103 1104 20 3.6396753396114008 1104 21 2.7106935434796817 1104 22 4.87262104604774 1104 23 5.052700532515196 1104 24 6.2372696317939 1104 25 5.19852916055941 1105 1 3.4430825977187567 1105 2 8.878646205853066 1105 3 5.884114869130094 1105 4 5.631319037217892 1105 5 1.434030125372086 1105 6 4.340176561161499 1105 7 5.47105821576903 1105 8 2.546913045593719 1105 9 3.70045915732236 1105 10 3.201069066967367 1105 11 6.368699981766209 1105 12 4.092146337735134 1105 13 4.637741178256267 1105 14 3.775557394928608 1105 15 4.051975392637807 1105 16 4.440983999460755 1105 17 4.830969210838006 1105 18 2.248559212522821 1105 19 2.2389958151473444 1105 20 1.2926368450019687 1105 21 2.499393343249988 1105 22 3.578807271687964 1105 23 0.8224386404949193 1105 24 4.122413400321161 1105 25 5.063678521087102 1106 1 6.938613247695322 1106 2 11.330963913129274 1106 3 7.920289628059752 1106 4 9.621110866105003 1106 5 8.917677935719013 1106 6 7.937035358337667 1106 7 7.793815329788938 1106 8 7.494733356392711 1106 9 6.700882068322844 1106 10 6.304025384615599 1106 11 8.648882578941635 1106 12 5.841227262825271 1106 13 4.907641059796902 1106 14 6.267796276908392 1106 15 6.466261370518012 1106 16 6.376728097711911 1106 17 6.556071156007189 1106 18 6.721263001377306 1106 19 6.588340229290139 1106 20 6.044883329046858 1106 21 6.2626436320177525 1106 22 6.698335353199816 1106 23 7.132136439955396 1106 24 8.0546568583013 1106 25 6.873728746381314 1107 1 3.714795189016403 1107 2 -13.146289305754845 1107 3 -5.182274628014127 1107 4 1.5111273674464378 1107 5 3.9644594257789194 1107 6 4.588891478614109 1107 7 4.470974255293427 1107 8 4.850587590801563 1107 9 3.198330534371614 1107 10 2.808259232197088 1107 11 5.372796053540419 1107 12 2.7116457084063903 1107 13 4.297316683087367 1107 14 3.4776791211259983 1107 15 3.247140768537104 1107 16 2.8872481706021915 1107 17 3.5249969972575492 1107 18 -1.2916319924674298 1107 19 1.436127515399452 1107 20 1.9195442283512563 1107 21 -1.3999028130844167 1107 22 1.6476810993335995 1107 23 -1.4289855015901942 1107 24 2.0356574906656313 1107 25 2.9340637872230673 1108 1 5.211296007843867 1108 2 10.366396608505873 1108 3 0.6536372299957769 1108 4 6.058200221351907 1108 5 7.2586171852656065 1108 6 7.488553650310827 1108 7 7.4945749451374475 1108 8 7.136496054510381 1108 9 5.639280755611395 1108 10 4.72474264474349 1108 11 7.036130203031544 1108 12 2.5218989385224617 1108 13 3.5490853427808418 1108 14 5.121504048415492 1108 15 5.041973955801076 1108 16 5.185149577215814 1108 17 0.7632197259552598 1108 18 4.073581837059326 1108 19 4.845415391032258 1108 20 5.273676618297717 1108 21 4.89058681293988 1108 22 1.5608701329188754 1108 23 4.650978129808539 1108 24 5.61267036841511 1108 25 6.073227843328958 1109 1 3.2904297034925065 1109 2 -13.146289305754845 1109 3 -5.311746046908738 1109 4 1.7408214457434674 1109 5 2.315942849483232 1109 6 2.343139670924419 1109 7 3.5393447903566084 1109 8 3.734028472623546 1109 9 3.0666932909403926 1109 10 2.4017486323779345 1109 11 0.08249778069028224 1109 12 1.2812275819215913 1109 13 2.199217714710187 1109 14 2.90619313700061 1109 15 3.0231857606451733 1109 16 2.6894305244394197 1109 17 3.4556403759762375 1109 18 3.5232374098525057 1109 19 2.11873415369622 1109 20 1.0579981877669624 1109 21 0.1047683348593047 1109 22 2.5988961649808537 1109 23 3.036462588303273 1109 24 3.9683540678039897 1109 25 4.275741446414816 1110 1 3.8200125046541267 1110 2 9.285572498467804 1110 3 6.49429966158722 1110 4 7.47008815474603 1110 5 8.545635890461046 1110 6 3.975876971939769 1110 7 6.569207650598209 1110 8 7.363376523626407 1110 9 5.728954527196091 1110 10 5.542605862896885 1110 11 3.356252855646222 1110 12 5.975931076059972 1110 13 10.34194616799919 1110 14 7.807969104007153 1110 15 7.121158275611386 1110 16 6.749925069530715 1110 17 7.452372425437883 1110 18 7.414255183793095 1110 19 6.106397283650768 1110 20 6.367768503645063 1110 21 4.496717260645433 1110 22 6.58875160454874 1110 23 6.757875156355848 1110 24 7.841367788915093 1110 25 8.17225337300723 1111 1 4.981405954291642 1111 2 9.229506872589106 1111 3 7.281207669041212 1111 4 7.905714504996386 1111 5 8.387269097407163 1111 6 7.524074687888135 1111 7 7.709106249743481 1111 8 7.915665378090157 1111 9 6.251172422019337 1111 10 5.687682786779877 1111 11 7.589621526170291 1111 12 6.774824329218049 1111 13 8.436769764393748 1111 14 6.017899960709897 1111 15 5.728425569731499 1111 16 5.558782013288692 1111 17 7.045891398365633 1111 18 6.741373363118569 1111 19 5.4159205110132795 1111 20 5.313793418984254 1111 21 7.93527071789762 1111 22 7.379528947063921 1111 23 7.280551503200563 1111 24 7.038668074115911 1111 25 7.472529841407223 1112 1 -2.5757166345331832 1112 2 6.865085101828093 1112 3 5.723210523855371 1112 4 -0.651907460685468 1112 5 3.7361308372913307 1112 6 3.2971557740974866 1112 7 5.577551066013335 1112 8 5.938973943826351 1112 9 4.39846358287647 1112 10 3.2920814430335135 1112 11 -0.53868123937702 1112 12 3.8052449183792447 1112 13 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4.581931586006444 2204 9 3.0197214817706275 2204 10 2.703506041506394 2204 11 4.1748892253412535 2204 12 4.532479647183877 2204 13 2.4872346756767314 2204 14 3.701754759264137 2204 15 3.6765088967459576 2204 16 3.800983730759121 2204 17 -0.9811755058907607 2204 18 2.4926134424836484 2204 19 3.2260406644355832 2204 20 3.344269356705418 2204 21 4.662999050472688 2204 22 4.367977252341447 2204 23 2.9705387706659323 2204 24 4.603880451529722 2204 25 4.573316699409446 2205 1 5.623708603556224 2205 2 9.512625422927325 2205 3 6.573052341866482 2205 4 7.122182533640391 2205 5 7.29324657752181 2205 6 6.771407828422284 2205 7 7.000763609538893 2205 8 6.6862664804939556 2205 9 5.625099388747369 2205 10 5.1787227189396425 2205 11 7.2357002180199785 2205 12 6.2069790011890476 2205 13 9.37380202063797 2205 14 6.760520101641397 2205 15 5.7947461383480245 2205 16 5.402963435754813 2205 17 6.210173546650154 2205 18 5.906871086755828 2205 19 4.61540732033567 2205 20 4.822897694345382 2205 21 -1.542111037813271 2205 22 3.130702004710095 2205 23 4.385920176781351 2205 24 6.391092445500437 2205 25 6.532491171847003 2206 1 3.404978305288644 2206 2 8.030686639262338 2206 3 5.120860399264565 2206 4 0.7666744842339952 2206 5 4.8633470064091 2206 6 5.127264404250088 2206 7 5.275187097999783 2206 8 5.1445121272435586 2206 9 3.5619250900180504 2206 10 2.9712340626976896 2206 11 2.615440843325217 2206 12 3.8398608041140037 2206 13 6.716965131003192 2206 14 -0.4128196250428502 2206 15 1.1441760645580992 2206 16 2.1803061104842065 2206 17 3.3686598320244734 2206 18 -1.6093875598073026 2206 19 0.10582839353065765 2206 20 1.4246674093972835 2206 21 2.379628131228703 2206 22 -2.023748477899101 2206 23 -3.611067400582634 2206 24 0.2719252682536606 2206 25 1.6112337240371666 2207 1 1.2743827789895583 2207 2 -13.146289305754845 2207 3 -5.7006403850384935 2207 4 -4.391897540174604 2207 5 0.9970502769075154 2207 6 2.151586866145653 2207 7 3.290621547491572 2207 8 3.5436249127434714 2207 9 1.217253363916626 2207 10 1.0803360583636206 2207 11 -1.2578307431430638 2207 12 -2.129340566829608 2207 13 0.4516077517541284 2207 14 -5.053781098988573 2207 15 -1.2245075756961405 2207 16 -0.05404182992689499 2207 17 1.0141979183250136 2207 18 1.126220705749553 2207 19 0.7008945379330918 2207 20 0.4907256745007835 2207 21 -3.0937690343997843 2207 22 -3.814554904650638 2207 23 -0.8392791896096448 2207 24 0.8631106992576605 2207 25 1.345815141948293 2208 1 4.373595223390534 2208 2 5.947223752374892 2208 3 5.760882459556907 2208 4 7.685480593950658 2208 5 2.8724432581859354 2208 6 5.8903753686785905 2208 7 6.0979701399461845 2208 8 6.203634144978643 2208 9 4.643097991178095 2208 10 4.130579867912836 2208 11 6.311372861726163 2208 12 5.813419575106746 2208 13 8.381802125129134 2208 14 2.3842343705624636 2208 15 4.001190195304414 2208 16 4.68336183733031 2208 17 5.449271816958628 2208 18 0.36515480492865393 2208 19 2.9928584181096447 2208 20 4.191883556836466 2208 21 6.482745522671507 2208 22 5.492036066579022 2208 23 1.7159308363310082 2208 24 2.6900322230358533 2208 25 4.004286903237961 2209 1 2.8877113437186246 2209 2 4.648145164622175 2209 3 4.960400275997433 2209 4 0.6527433855030651 2209 5 4.878807839171753 2209 6 5.172903474625873 2209 7 5.556969412435858 2209 8 3.8470376228107526 2209 9 3.6559610089998316 2209 10 2.7871276877262225 2209 11 -0.8800538601365062 2209 12 2.5987510914288414 2209 13 1.9316214575438764 2209 14 3.258745645460028 2209 15 3.616825012345398 2209 16 3.039748878874022 2209 17 0.7953118769139493 2209 18 2.732268464426596 2209 19 3.210573067687129 2209 20 3.5130181099469873 2209 21 5.55229157740267 2209 22 4.28650003061793 2209 23 -0.1951102294338085 2209 24 -2.86013498333962 2209 25 -0.28404011038508514 2210 1 -2.18074180649549 2210 2 -13.146289305754845 2210 3 -7.24934097263162 2210 4 -2.1709717644455893 2210 5 -0.9033771568813123 2210 6 -1.310585756874172 2210 7 -1.1892652800873158 2210 8 -19.847755913993556 2210 9 -12.4423002432618 2210 10 -6.8103389379575034 2210 11 -9.803341832808336 2210 12 -4.480666159128269 2210 13 -5.105694555840778 2210 14 -3.7248658362827705 2210 15 -5.380333542726589 2210 16 -4.8295184579568335 2210 17 -4.373023214494307 2210 18 -4.560044091022618 2210 19 -9.580139756014049 2210 20 -6.730011879783991 2210 21 -4.805173371706299 2210 22 -9.215345948047657 2210 23 -7.309721578447771 2210 24 -9.94596687877724 2210 25 -7.550936840365736 2211 1 -0.06656379093128706 2211 2 4.3139379669724285 2211 3 0.30250285474722816 2211 4 1.3176351215348383 2211 5 0.35869025851781444 2211 6 -0.400558932207268 2211 7 -19.522922867635955 2211 8 -12.764422580660222 2211 9 -8.971755790956646 2211 10 -7.3988455999077445 2211 11 -9.637058375668849 2211 12 -4.993404191199961 2211 13 -6.753532369777814 2211 14 -4.889233245786368 2211 15 -5.237237946688385 2211 16 -11.387699981362092 2211 17 -7.735594697809951 2211 18 -5.9270519615567565 2211 19 -8.705382946675527 2211 20 -6.9290892291531705 2211 21 -4.628904358618222 2211 22 -6.080176156749463 2211 23 -7.6507522646876005 2211 24 -20.845772326248056 2211 25 -20.582065245034997 2212 1 -1.3778594298765192 2212 2 -13.146289305754845 2212 3 -7.574514017832525 2212 4 -18.81494063604092 2212 5 -18.84112034754279 2212 6 -19.48957408372242 2212 7 -9.447872157696807 2212 8 -9.225037861255219 2212 9 -5.139402081447763 2212 10 -5.635192332958333 2212 11 -5.95434892825352 2212 12 -3.1331235193700984 2212 13 -5.834218797954101 2212 14 -4.0115210323743575 2212 15 -3.966199084435022 2212 16 -4.225401284453859 2212 17 -4.296133529330021 2212 18 -3.391341791908957 2212 19 -3.9453677336937822 2212 20 -4.024800397633101 2212 21 -1.8708022933406987 2212 22 -3.1632252070906013 2212 23 -3.2248406382815524 2212 24 -2.2859345980533305 2212 25 -3.030341107103961 2213 1 -17.910313790931287 2213 2 -13.146289305754845 2213 3 -8.061329433654027 2213 4 -5.524618055395758 2213 5 -18.84112034754279 2213 6 -9.904479534222977 2213 7 -19.522922867635955 2213 8 -10.061326548031376 2213 9 -8.133840346186364 2213 10 -6.7262084947066825 2213 11 -5.985560077896913 2213 12 -4.884121360108175 2213 13 -18.14557782432327 2213 14 -11.636561927486573 2213 15 -6.6297795332997715 2213 16 -5.895753963901612 2213 17 -4.509606015098523 2213 18 -4.543864202430452 2213 19 -5.327393513972524 2213 20 -5.311884085390741 2213 21 -3.444577075778703 2213 22 -4.307764891707496 2213 23 -4.082263919051037 2213 24 -3.18392583014127 2213 25 -4.13490173001831 2214 1 -17.910313790931287 2214 2 -13.146289305754845 2214 3 -11.722981233236862 2214 4 -18.81494063604092 2214 5 -18.84112034754279 2214 6 -19.48957408372242 2214 7 -15.795767140480228 2214 8 -14.309789376027018 2214 9 -13.518446150147005 2214 10 -13.525074101136248 2214 11 -13.80975054836102 2214 12 -11.916992146037915 2214 13 -18.14557782432327 2214 14 -15.983067339620462 2214 15 -22.62644249214293 2214 16 -17.486148267310377 2214 17 -22.297147728112982 2214 18 -17.011522389777184 2214 19 -14.349607528400108 2214 20 -13.156347331411272 2214 21 -10.728420270634134 2214 22 -11.757475209048515 2214 23 -11.6679110630964 2214 24 -10.59995508043081 2214 25 -10.349118012087764 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 126077.0 3 1.0 4 675.0 5 2552.0 6 3064.0 7 2845.0 8 2481.0 9 2489.0 10 2249.0 11 2224.0 12 2092.0 13 2265.0 14 3180.0 15 6130.0 16 8803.0 17 9247.0 18 10134.0 19 11710.0 20 14052.0 21 16432.0 22 17441.0 23 18984.0 24 19693.0 25 19650.0 26 16693.0 27 14036.0 28 15226.0 29 17154.0 30 16711.0 31 19495.0 32 24440.0 33 30174.0 34 36132.0 35 39676.0 36 38042.0 37 36986.0 38 21590.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.63607164047748 17.708132323157635 11.858082457605263 25.79771357875962 2 15.65007165069555 19.60450765736345 39.19682982228678 25.548590869654227 3 20.082997379644628 23.311989084910522 27.952069680436736 28.652943855008118 4 12.145573965829788 15.968016739988883 37.140760492463734 34.74564880171759 5 14.531814996623035 36.6783698582761 34.713392519252324 14.076422625848542 6 33.464882869612055 35.483930069878824 16.94075873668473 14.11042832382439 7 30.22645133614798 30.26066478290092 20.841893931368176 18.670989949582925 8 26.097829656795664 37.10657397777656 18.762486999835787 18.033109365591987 9 26.50322519220241 15.60913685446568 18.758479518244354 39.129158435087554 10 14.949438508941789 28.848469667922274 33.37315311670086 22.82893870643507 11 34.327287289959784 22.144370838825942 23.24600777586811 20.28233409534616 12 23.99293888157763 24.02517151770624 30.571569812883702 21.410319787832428 13 28.465086728182342 20.57270138270935 24.5655231092476 26.396688779860707 14 21.670827713374884 21.045786518208665 26.620517363059232 30.66286840535722 15 23.299890150128157 29.802839822612796 22.99198502786932 23.90528499938972 16 23.557543616338993 27.6587328716952 25.74885974079247 23.034863771173335 17 22.68477622526651 27.533947288365272 27.307821826150192 22.473454660218025 18 22.576711756578735 27.04607524981525 28.20004498281014 22.177168010795874 19 23.42575538852108 26.70182205315379 26.212387135312376 23.660035423012754 20 23.702338802887773 26.839555673556468 27.098380437426567 22.35972508612919 21 22.930253578367825 25.40962786294167 26.15746467815307 25.50265388053744 22 23.42722619673602 26.48682460735931 27.099483983218605 22.98646521268607 23 23.232211033427802 26.613074703980043 27.151639019095708 23.003075243496447 24 23.06323956482164 26.60378830566512 26.89419617701877 23.438775952494474 25 23.43897032103679 26.355769886873055 26.903711179557487 23.30154861253267 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 594.0 1 594.0 2 1178.0 3 1762.0 4 1762.0 5 1762.0 6 2552.5 7 3343.0 8 3343.0 9 3343.0 10 3928.5 11 4514.0 12 4514.0 13 4514.0 14 5276.0 15 6038.0 16 6038.0 17 6038.0 18 8364.5 19 10691.0 20 10691.0 21 10691.0 22 15029.5 23 19368.0 24 19368.0 25 19368.0 26 25313.5 27 31259.0 28 31259.0 29 31259.0 30 38374.5 31 45490.0 32 45490.0 33 45490.0 34 51878.5 35 58267.0 36 58267.0 37 58267.0 38 63284.0 39 68301.0 40 68301.0 41 68301.0 42 71595.5 43 74890.0 44 74890.0 45 74890.0 46 76369.5 47 77849.0 48 77849.0 49 77849.0 50 75063.5 51 72278.0 52 72278.0 53 72278.0 54 65452.5 55 58627.0 56 58627.0 57 58627.0 58 50611.5 59 42596.0 60 42596.0 61 42596.0 62 35171.5 63 27747.0 64 27747.0 65 27747.0 66 21633.0 67 15519.0 68 15519.0 69 15519.0 70 11431.0 71 7343.0 72 7343.0 73 7343.0 74 5121.0 75 2899.0 76 2899.0 77 2899.0 78 1967.5 79 1036.0 80 1036.0 81 1036.0 82 667.0 83 298.0 84 298.0 85 298.0 86 193.5 87 89.0 88 89.0 89 89.0 90 54.5 91 20.0 92 20.0 93 20.0 94 12.0 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.100661831728292 2 34.95438513058296 3 4.959854159235921 4 12.727301549558117 5 12.452581936353187 6 13.478064439424564 7 8.723179962747196 8 7.326437601553522 9 0.09986921888003805 10 1.2711924860301986 11 4.999009233939682 12 5.081123925018825 13 14.668727460072128 14 1.3927792969524035 15 2.5918440137914636 16 2.6141956961122337 17 2.5557008679110687 18 1.326041295129394 19 1.2786430468037888 20 0.2897792573217612 21 3.8906194269409102 22 1.4181429080965402 23 0.1000277414496889 24 8.56814488962866 25 0.10272262513375341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 630825.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.97795316457471 #Duplication Level Percentage of deduplicated Percentage of total 1 96.781139807405 90.95293424034463 2 2.3157300050129477 4.352551319058143 3 0.4637408648049796 1.3074425181942524 4 0.16816947143596112 0.6321689084128015 5 0.08975929556525211 0.42176974373582404 6 0.045649203832421596 0.2574011243858065 7 0.030131223369920798 0.1982169489054829 8 0.021777623826792335 0.1637293209624013 9 0.016874645736148462 0.14272601999944953 >10 0.061713166323963786 1.0053803029669013 >50 0.003085979081117366 0.19237530876496217 >100 0.0022287136055417467 0.3733042442693796 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5852256965085405E-4 0.0 10 1.5852256965085405E-4 0.0 0.0 1.5852256965085405E-4 0.0 11 1.5852256965085405E-4 0.0 0.0 1.5852256965085405E-4 0.0 12 1.5852256965085405E-4 0.0 0.0 1.5852256965085405E-4 0.0 13 1.5852256965085405E-4 0.0 0.0 1.5852256965085405E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAC 150 0.0 16.745861 1 GCACCGT 75 2.048182E-8 16.545845 6 GTAGGAC 185 0.0 16.411001 3 TTTGGAC 40 0.0021859321 16.264475 3 GTATAGG 50 0.0025800029 15.8645 1 CTAGCAC 65 1.5640524E-5 15.013361 3 TGTAGGA 200 0.0 14.882538 2 TAGGACC 95 1.638E-8 14.574374 4 AGCACCG 50 7.8664324E-4 14.482238 5 TTATACC 45 0.0053942064 14.200672 4 GTCCTAA 75 1.644311E-4 14.101777 1 GTCTATG 90 5.1791907E-5 13.220417 1 CCTACAG 250 0.0 13.0115795 3 TCCTACA 220 0.0 12.914598 2 GGACTGT 90 2.8807754E-6 12.639188 6 CTAGGCT 60 0.002495866 12.425588 4 GATTAAA 75 0.0026285136 12.339055 1 CTGTAGG 215 1.8189894E-12 12.298061 1 CTATAGT 70 5.709965E-4 12.172005 4 AGGACGT 170 1.8189894E-12 12.169948 5 >>END_MODULE