FastQCFastQC Report
Thu 26 May 2016
SRR1926607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630825
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15130.23984464788174217No Hit
GGTATCAACGCAGAGTACTTTTTTT8840.14013395157135497No Hit
TATCAACGCAGAGTACTTTTTTTTT6890.10922205048943844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC551.0867929E-515.6039381
TTAGGCC500.001453390313.3532314
GTGTTAC500.001456015413.3500371
TCCAACG500.001528448513.26432318
GTGCTAG1001.3684803E-712.3964621
ATAGGAC853.7575883E-612.3443093
GCTAGGC701.0482579E-412.2641513
GTCATAG701.05138744E-412.2602371
TTTAGGA1103.583773E-812.141242
TAGGACC1650.012.1393024
TGTCCAA658.1400585E-411.67019410
TAGGACG1800.011.6575834
TTAGGAC1354.4092303E-911.3052253
CTTACAC1104.6955392E-711.27310853
TAGGCTG1104.700396E-711.2722095
TGGCGAG2450.011.21473418
TTAGGAG600.005693711.1285813
CTAGACT600.005697084611.1276934
TAAGGGT600.005697084611.1276934
TAGGATC600.005697084611.1276934
Produced by FastQC (version 0.11.5)