##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926607_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 630825 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.341490904767568 31.0 28.0 31.0 22.0 34.0 2 28.798229302897 31.0 28.0 33.0 23.0 34.0 3 29.16188958903024 31.0 28.0 33.0 25.0 34.0 4 33.02316807355447 35.0 33.0 37.0 27.0 37.0 5 32.7591392224468 35.0 33.0 37.0 26.0 37.0 6 32.874001505964415 35.0 33.0 37.0 26.0 37.0 7 32.702838346609596 35.0 33.0 37.0 25.0 37.0 8 32.673744699401574 35.0 33.0 37.0 25.0 37.0 9 33.92747909483613 37.0 34.0 39.0 25.0 39.0 10 33.81819680577022 37.0 33.0 39.0 25.0 39.0 11 34.06545713946023 37.0 34.0 39.0 26.0 39.0 12 33.881426703126856 37.0 34.0 39.0 25.0 39.0 13 33.99814845638648 37.0 34.0 39.0 26.0 39.0 14 34.66427773154203 38.0 34.0 40.0 24.0 41.0 15 34.77449371854318 38.0 34.0 40.0 25.0 41.0 16 34.739361946657155 38.0 34.0 40.0 25.0 41.0 17 34.64436729679388 38.0 34.0 40.0 24.0 41.0 18 34.615513018666036 38.0 34.0 40.0 24.0 41.0 19 34.64820829865652 39.0 34.0 40.0 24.0 41.0 20 34.628386636547376 39.0 34.0 40.0 24.0 41.0 21 34.55472753933341 39.0 34.0 40.0 22.0 41.0 22 34.48152340189434 39.0 34.0 40.0 22.0 41.0 23 34.150494986723736 39.0 34.0 40.0 18.0 41.0 24 34.2111219434867 39.0 34.0 40.0 18.0 41.0 25 34.0162073475211 39.0 34.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8028278023649484 1101 2 0.8481947804263292 1101 3 0.8196429224140367 1101 4 0.7358311723191804 1101 5 0.6797417169374924 1101 6 0.4071779436840899 1101 7 0.7115652952793496 1101 8 0.6820735451778148 1101 9 1.200281977114365 1101 10 1.0801943281240227 1101 11 0.8597008034530589 1101 12 0.8977664645602346 1101 13 0.8864790096937369 1101 14 1.1580339419644972 1101 15 0.9257550090019819 1101 16 0.9177512120652693 1101 17 1.0645627947260508 1101 18 1.0188743588616305 1101 19 1.0320232805346592 1101 20 1.1029225999608983 1101 21 1.1609084511456658 1101 22 0.8751069757025078 1101 23 1.3321269201367656 1101 24 1.2829123578879944 1101 25 1.5920382471788983 1102 1 0.5689730825224437 1102 2 0.6207292533066067 1102 3 0.4953642165275127 1102 4 0.41936986716955005 1102 5 0.607787812124684 1102 6 0.213883727549117 1102 7 0.4878620243659526 1102 8 0.5544685941573491 1102 9 0.8009264824010742 1102 10 0.8111288456924157 1102 11 0.7268955749435477 1102 12 0.7804420446942544 1102 13 0.5392772632338705 1102 14 0.6374694827548524 1102 15 0.6506053000408798 1102 16 0.7634020846535634 1102 17 0.7295340305260609 1102 18 0.742528940865455 1102 19 0.8704817821089392 1102 20 0.8783474578760888 1102 21 0.8290410691672037 1102 22 0.9820142335204736 1102 23 1.2506564093863304 1102 24 1.1312090386631226 1102 25 1.1208564201445554 1103 1 0.5923053077126283 1103 2 0.3681353846703921 1103 3 0.2589965082837793 1103 4 0.24095417090306626 1103 5 0.23137483274621928 1103 6 -0.03060209790516666 1103 7 0.3874518564163978 1103 8 0.43663655611162255 1103 9 0.6920944265320657 1103 10 0.6802687638758087 1103 11 0.49024269419757616 1103 12 0.5802811702065753 1103 13 0.46201333840782155 1103 14 0.8539952292597448 1103 15 0.6803562313073357 1103 16 0.7159890564548093 1103 17 0.7077129096276451 1103 18 0.6702315121450226 1103 19 0.7537206907971665 1103 20 0.5657945207930482 1103 21 0.653726623862589 1103 22 0.6218878587061667 1103 23 1.0400751146876743 1103 24 0.7291088361751505 1103 25 0.8313637094791773 1104 1 0.5525306448201626 1104 2 0.6589521852303264 1104 3 0.6085547224345511 1104 4 0.5047489456588679 1104 5 0.5848089318623408 1104 6 0.09413001232296381 1104 7 0.4860740048614005 1104 8 0.3935690169191872 1104 9 0.6816002705551796 1104 10 0.8360877013599364 1104 11 0.4553659234926357 1104 12 0.44259124719778953 1104 13 0.39293803815449024 1104 14 0.842798056193665 1104 15 0.6058008044489824 1104 16 0.6486124560844075 1104 17 0.7923234939060748 1104 18 0.759863094026386 1104 19 0.6394517666301027 1104 20 0.7268659560008928 1104 21 0.7196460896934838 1104 22 0.8270805844923359 1104 23 0.9297114969059876 1104 24 0.576074868371478 1104 25 0.7433487572038189 1105 1 1.0073196219651166 1105 2 1.0313403301894084 1105 3 1.0816056397030103 1105 4 0.829923019220729 1105 5 1.032760485897299 1105 6 0.6876885389210372 1105 7 0.7596506002350694 1105 8 0.7003135406671532 1105 9 1.4361941289753517 1105 10 1.1989694455830389 1105 11 1.0281866968723108 1105 12 0.9394908437319529 1105 13 1.079052439660117 1105 14 1.50240306855018 1105 15 1.2145546902879971 1105 16 1.2514616704985428 1105 17 1.1849277726883471 1105 18 1.4472627536960374 1105 19 1.5764433004617047 1105 20 1.4889263375797555 1105 21 1.28479590118895 1105 22 1.435254225125135 1105 23 1.6881288104608743 1105 24 1.3414671502082953 1105 25 1.5865698095822225 1106 1 0.37513336398940567 1106 2 0.6962612281187681 1106 3 0.3561184248265796 1106 4 0.3548382340528775 1106 5 0.580892998010782 1106 6 -0.7019630576971494 1106 7 -0.5172889412054076 1106 8 -0.34554905385124357 1106 9 -0.3597620152277088 1106 10 -0.33894456251353233 1106 11 -0.475557873740172 1106 12 -0.30456127838821345 1106 13 -0.45992069109909295 1106 14 -0.2752261942340226 1106 15 -0.26496512490063395 1106 16 -0.3731003804352966 1106 17 -0.4453284464282632 1106 18 -0.42376636664170775 1106 19 -0.3342936939179353 1106 20 -0.3152031337076835 1106 21 -0.2999313285963794 1106 22 -0.24662961089168078 1106 23 0.14203704689248298 1106 24 -0.25129465052165045 1106 25 -0.05606449049268747 1107 1 0.6428696867721087 1107 2 0.7540406190284372 1107 3 0.6679533665106163 1107 4 0.5856250225026898 1107 5 0.5257029332402112 1107 6 0.17204152975060794 1107 7 0.537398225540457 1107 8 0.5864856251583817 1107 9 0.8444864253768216 1107 10 0.4216011417120882 1107 11 0.2748295897304942 1107 12 0.36718550646946824 1107 13 0.3015716887236266 1107 14 0.6589023964915555 1107 15 0.7586912879846324 1107 16 0.41616813493040894 1107 17 0.4804708957631547 1107 18 0.9023555187768721 1107 19 0.7796418947234542 1107 20 0.6794116666111165 1107 21 0.7535472364978517 1107 22 0.5478587165607891 1107 23 0.7461717367533254 1107 24 0.687186402870104 1107 25 0.7054465983339213 1108 1 1.502481393983512 1108 2 1.2287782471823725 1108 3 1.281890900691991 1108 4 1.0492911270120757 1108 5 -0.07900133923139663 1108 6 0.6312065621947838 1108 7 0.9673020943572084 1108 8 1.1975132135799242 1108 9 1.541083962716037 1108 10 1.6107788894369506 1108 11 1.308697849659211 1108 12 1.1977648793399212 1108 13 1.1245791005042136 1108 14 1.5709777942159207 1108 15 1.4266332965413184 1108 16 1.4344432045135846 1108 17 1.3008376175776846 1108 18 1.299834277698352 1108 19 1.4409104785218858 1108 20 1.4802722172811684 1108 21 1.5149553298846143 1108 22 1.5471935861014003 1108 23 1.7475942921509855 1108 24 1.7550060640349514 1108 25 1.6886604446414921 1109 1 0.2662592953517873 1109 2 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1110 15 0.9902316327377747 1110 16 0.7070228556656346 1110 17 0.9090486593356601 1110 18 0.9211686790363842 1110 19 1.27847804745101 1110 20 1.3675784105329214 1110 21 1.2752344552626553 1110 22 1.3070176340071171 1110 23 1.511941407110946 1110 24 1.5279677451683824 1110 25 1.6618788835074625 1111 1 -0.09103916117002342 1111 2 -0.12721387800580075 1111 3 0.020789780370247257 1111 4 -0.06165041930737658 1111 5 0.07427090301221284 1111 6 -0.34305620909893264 1111 7 -0.01805085117428007 1111 8 0.07861883970786465 1111 9 0.30083931176874046 1111 10 0.17297692678419452 1111 11 -0.13869447470788998 1111 12 -0.05117056423918598 1111 13 0.054410323830595075 1111 14 0.25219280157567425 1111 15 0.12128691069942477 1111 16 0.0664509869802643 1111 17 -0.08991130950706605 1111 18 0.10692208598983655 1111 19 0.043481653940240506 1111 20 -0.19538058726476493 1111 21 0.1934684456814466 1111 22 -0.09829499534184549 1111 23 0.2789394982040534 1111 24 0.04406945340427626 1111 25 0.09649401958475323 1112 1 -0.18240787637428113 1112 2 -0.14891909041346096 1112 3 0.1966773817657419 1112 4 0.2503816305095654 1112 5 0.16544642233395734 1112 6 -0.05356080396975216 1112 7 0.12293643153425649 1112 8 0.11663185455548586 1112 9 -0.1281650825751015 1112 10 0.1640597293319601 1112 11 -0.07932900243451257 1112 12 0.14358468503956345 1112 13 -0.19317537449920508 1112 14 0.32008735457301185 1112 15 0.08904907276833995 1112 16 0.007100176894404342 1112 17 0.03745738844755664 1112 18 -0.03762638332091228 1112 19 0.03185971413283539 1112 20 -0.2231257584458035 1112 21 0.05843573606606611 1112 22 -0.10094281391020843 1112 23 0.04986610533073588 1112 24 -0.19441447640696907 1112 25 -0.04933178500278501 1113 1 -0.0882704501093734 1113 2 -0.15988188589732033 1113 3 -1.5386521736255538 1113 4 -1.2372395704122177 1113 5 -1.5556883129927037 1113 6 -0.7068945237933164 1113 7 -0.590252064583936 1113 8 -0.7073083167563077 1113 9 -0.0619805624751919 1113 10 -0.0559198833081993 1113 11 -0.3168890895880665 1113 12 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1.172292710872739 2202 24 1.2186476570285976 2202 25 0.9827268489915539 2203 1 0.3969458980751952 2203 2 0.5006178860120514 2203 3 0.12987019333565897 2203 4 0.029499747343372462 2203 5 0.41202982330975146 2203 6 0.3684880207969883 2203 7 0.731502149460816 2203 8 0.5037684399639204 2203 9 0.9455154529786469 2203 10 0.9598240018918176 2203 11 0.655906175137865 2203 12 0.6815238155502428 2203 13 0.47158682951046416 2203 14 0.9781088779685732 2203 15 0.8574299303875037 2203 16 1.026939929715752 2203 17 1.0717298610196266 2203 18 0.9457283337590283 2203 19 0.9625676470664217 2203 20 0.9113177840991256 2203 21 1.082657847732861 2203 22 1.1274225077624322 2203 23 1.292254800747635 2203 24 1.1461160150700778 2203 25 1.0568782295730728 2204 1 0.4862549066694264 2204 2 0.5769300407081843 2204 3 0.4896432519605831 2204 4 0.28748010339002406 2204 5 0.23401129200220083 2204 6 0.3996612866042355 2204 7 0.40677743337732863 2204 8 0.5330952892579646 2204 9 0.7413929084728181 2204 10 0.7173583404430772 2204 11 0.650793759545472 2204 12 0.5629838754029137 2204 13 0.6610579390868878 2204 14 0.9179061078642192 2204 15 0.8595401206883011 2204 16 0.5638586682056186 2204 17 0.6665829150405003 2204 18 0.6396404347412243 2204 19 0.6936718521001879 2204 20 0.8569468126442459 2204 21 0.7567053845652794 2204 22 1.0608364119981601 2204 23 1.1391556985376852 2204 24 0.8719543361259454 2204 25 0.7503539048339647 2205 1 0.388453980743499 2205 2 0.48471427843428216 2205 3 0.24197825411389928 2205 4 -0.015491472837361187 2205 5 0.06068953274294131 2205 6 -0.0548229941881857 2205 7 -0.28975976515841495 2205 8 -0.12446688774462444 2205 9 0.37012083698272136 2205 10 0.29084698170061074 2205 11 0.17363236850327013 2205 12 0.28774297316346775 2205 13 0.16510213589998557 2205 14 0.35678557599513994 2205 15 0.36842254962886756 2205 16 0.2985028327189738 2205 17 0.443539741052561 2205 18 0.42379068237257655 2205 19 0.24053339387452155 2205 20 0.41899596208438084 2205 21 0.589022621860714 2205 22 0.4683245687595772 2205 23 0.7244741201569838 2205 24 0.5228345040845994 2205 25 0.5501089651268174 2206 1 0.4547006397736624 2206 2 0.6295503156222715 2206 3 0.772244554938819 2206 4 0.6978466555091529 2206 5 0.9694333280846763 2206 6 0.6795510345996547 2206 7 0.864189016997031 2206 8 0.7594072165202803 2206 9 1.0743861788050069 2206 10 1.0178397980604856 2206 11 0.973262974894844 2206 12 0.9311915644063546 2206 13 0.7139735318703941 2206 14 0.8933231324919362 2206 15 0.6803448171373248 2206 16 0.8985343554908454 2206 17 0.9146320272970883 2206 18 1.0092262738019357 2206 19 0.8857179574839193 2206 20 1.098114123640812 2206 21 1.03771376085394 2206 22 0.8040709480806356 2206 23 1.1214634734517759 2206 24 1.0035945136746278 2206 25 0.8010590881100299 2207 1 -0.7671211194226295 2207 2 0.19209055082252036 2207 3 0.46375504102537235 2207 4 0.6066680248206922 2207 5 0.5512592581411653 2207 6 0.6694593663462456 2207 7 0.7317256706977631 2207 8 0.966442822972418 2207 9 0.9575272898858955 2207 10 0.7017906000443617 2207 11 0.449656812902262 2207 12 0.7180039391672111 2207 13 0.398971582446606 2207 14 -4.916950330863429 2207 15 -1.611014701944356 2207 16 -0.43719948922621654 2207 17 -0.28628556194572496 2207 18 0.33357412354216365 2207 19 0.43933844547456147 2207 20 0.4128848623364192 2207 21 0.7497861343453991 2207 22 0.5769698772576177 2207 23 0.9929307095315281 2207 24 0.740216026246145 2207 25 0.685687201521155 2208 1 0.1876364825376271 2208 2 0.5853370892900429 2208 3 0.21360962961666985 2208 4 0.023111480344063295 2208 5 0.3106817321875397 2208 6 0.09263435128644204 2208 7 0.30504673390152703 2208 8 0.3547709363140328 2208 9 0.5293138459435625 2208 10 0.30677245047770896 2208 11 0.21828855267474268 2208 12 0.22184242988199543 2208 13 -0.13817112227227568 2208 14 0.17446954393086145 2208 15 0.3251806242921589 2208 16 -0.053826848503170766 2208 17 -0.01303511126297252 2208 18 0.21306974415805513 2208 19 -0.10856359206344024 2208 20 0.14121782149007345 2208 21 0.3871660171123139 2208 22 0.12039657768195866 2208 23 0.4940573237834016 2208 24 0.5893104743829838 2208 25 0.4930335711262046 2209 1 -0.2573336381141367 2209 2 -0.12440897784358285 2209 3 0.41797801906610843 2209 4 0.4197861077786129 2209 5 0.5335933162391839 2209 6 0.5289189666361551 2209 7 0.7439835100779106 2209 8 0.7788890380112079 2209 9 0.9066413874275341 2209 10 0.7742931225097038 2209 11 0.600564979373921 2209 12 0.5071368900647926 2209 13 0.603864454646434 2209 14 0.9373072274532817 2209 15 0.6105998653521709 2209 16 0.6056468938190278 2209 17 0.8281846255930603 2209 18 0.7666960276995312 2209 19 0.961731147931026 2209 20 0.8429348063605815 2209 21 0.8355831149662336 2209 22 1.057488865618751 2209 23 1.1803408047615065 2209 24 1.0783645445839483 2209 25 1.1632210751910392 2210 1 -0.29856864499507907 2210 2 -0.4226646415226867 2210 3 0.5183769041438957 2210 4 0.43252982236970894 2210 5 0.7285091032862496 2210 6 0.6922872053245115 2210 7 0.9491770496765071 2210 8 0.8948853076323715 2210 9 1.275203165714494 2210 10 1.2259274336121209 2210 11 1.038608556346162 2210 12 0.705199747646752 2210 13 0.810083149852133 2210 14 1.26195671854299 2210 15 0.9672195413541047 2210 16 1.0938724490097727 2210 17 1.0626256625545523 2210 18 1.2342787561852333 2210 19 1.077980650197894 2210 20 1.2245598963018338 2210 21 1.3105619831385553 2210 22 1.5560131305455016 2210 23 1.1820889832328874 2210 24 1.165694878711328 2210 25 1.2277709497972182 2211 1 -1.1947741337346152 2211 2 -0.1986381411099991 2211 3 0.3299999438797734 2211 4 0.6025053559152767 2211 5 0.843623034426443 2211 6 0.4798380542810037 2211 7 0.630814680852076 2211 8 0.5554279912781652 2211 9 0.9167401306950396 2211 10 0.9682263959986273 2211 11 0.635800072676787 2211 12 0.8378418299483741 2211 13 0.9141355906020436 2211 14 1.1744559816015894 2211 15 0.6594138580620381 2211 16 0.9715291227510789 2211 17 0.9385210463536282 2211 18 0.8947698539331412 2211 19 0.9793410440193782 2211 20 0.8732820399169725 2211 21 0.8961466724440683 2211 22 1.0826167150284647 2211 23 1.1474416833861696 2211 24 1.1912409522875578 2211 25 1.233150622005688 2212 1 0.7425971705864853 2212 2 -0.14310883691183207 2212 3 0.23986686870613028 2212 4 0.26037845813421967 2212 5 0.49743357902045915 2212 6 0.5442339145059734 2212 7 0.6523068592281263 2212 8 0.675007406116201 2212 9 0.7978253233928072 2212 10 0.27785283278024053 2212 11 0.5695909479610677 2212 12 0.8460668284117077 2212 13 0.6616865310409139 2212 14 0.37818508296000175 2212 15 0.41961731248456147 2212 16 -0.03666322158477442 2212 17 0.4095645186014636 2212 18 0.40647155655776857 2212 19 0.21920289785182234 2212 20 0.4760042416503296 2212 21 0.6233752206031369 2212 22 0.6618548366093862 2212 23 1.0582363695992143 2212 24 0.7717141682197024 2212 25 0.24600482324731132 2213 1 0.8066552743619226 2213 2 0.507941349569883 2213 3 0.07540308541750917 2213 4 -0.9058519940021412 2213 5 -1.652242531690792 2213 6 -0.21373245335374236 2213 7 0.04859685464787589 2213 8 -0.07415833359458901 2213 9 -0.4435212708622487 2213 10 -0.35117942528427903 2213 11 -0.2912348061448 2213 12 -0.7486789675707612 2213 13 -0.7248540696264953 2213 14 -7.91158132415902 2213 15 -3.445559681561111 2213 16 -2.1299040774376863 2213 17 -1.0066168594002392 2213 18 -5.554007406996508 2213 19 -3.1453197743983914 2213 20 -5.444299363662761 2213 21 -2.3321636133954193 2213 22 -1.7893327574493725 2213 23 -4.784628251221303 2213 24 -2.351625490224322 2213 25 -1.8691558127534762 2214 1 0.1616557539452721 2214 2 -0.021605995327853122 2214 3 -1.5101925213126925 2214 4 -1.8747893088793859 2214 5 -0.42007694802354223 2214 6 -0.8628735696806231 2214 7 -0.8665125977252011 2214 8 -0.7192809709307966 2214 9 -5.602725763770856 2214 10 -1.6694023041300703 2214 11 -0.8850076668809521 2214 12 -1.2086437790372386 2214 13 -1.5471849019684498 2214 14 -3.1706322072366007 2214 15 -2.278839330191154 2214 16 -1.5108879534160025 2214 17 -1.9541203775377909 2214 18 -1.1183195341562424 2214 19 -4.922064419886084 2214 20 -2.670029940895006 2214 21 -4.733229863182469 2214 22 -6.9570609496492 2214 23 -6.99140454921006 2214 24 -6.55360738631078 2214 25 -4.112131348276286 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18962.0 3 12.0 4 339.0 5 2243.0 6 2814.0 7 2390.0 8 1892.0 9 1438.0 10 1330.0 11 1243.0 12 1148.0 13 1196.0 14 1125.0 15 1190.0 16 1278.0 17 1300.0 18 1428.0 19 1753.0 20 2227.0 21 2782.0 22 3715.0 23 4563.0 24 5626.0 25 6422.0 26 7560.0 27 8432.0 28 9908.0 29 12110.0 30 14669.0 31 18575.0 32 23784.0 33 31422.0 34 40906.0 35 53762.0 36 72491.0 37 106039.0 38 162751.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.593964624149436 18.378530168153485 9.087649547767235 29.939855659929844 2 14.914582424987119 20.3976376392247 37.49748305521424 27.190296880573943 3 19.036883069112747 23.546152589964215 28.289756358972163 29.12720798195087 4 12.90210045256618 16.33265992838862 35.39482989917955 35.370409719865656 5 14.124335885414768 37.50751117361394 34.082098911256374 14.286054029714917 6 33.99168481866827 36.42163983776964 16.324072497688903 13.262602845873198 7 29.78704484428121 31.03879750443425 20.352453406631447 18.821704244653095 8 26.687491166526655 36.6170298249821 18.613895033722034 18.08158397476922 9 26.59333888108956 15.552086443358892 18.579319150558057 39.27525552499349 10 15.32902955780609 29.087582677514888 32.62123393218383 22.962153832495197 11 35.841943901054826 22.51345087458427 22.422457332906372 19.222147891454533 12 23.651812748688194 24.47821055468366 30.18547581680696 21.684500879821183 13 29.42908096540245 21.153251694209963 24.936234296357945 24.481433044029643 14 21.802402590089198 21.100153393940065 26.170877993918175 30.92656602205256 15 23.87757560769916 29.468376182187683 22.480351034047636 24.173697176065513 16 23.58955823675162 27.82171578581925 25.53942293516462 23.04930304226451 17 22.77965897489553 28.024235737249604 26.237626901541528 22.958478386313338 18 22.73593400252373 27.315903946025124 27.374717031381774 22.57344502006937 19 24.12716781332234 26.64531879141435 26.16578421736787 23.061729177895437 20 23.895964313229406 26.807403673302453 26.78568597797794 22.5109460354902 21 23.733561183467973 26.051481871330196 26.524355446210034 23.690601498991796 22 23.704196885031507 26.659216105893073 26.413030555225298 23.223556453850115 23 23.46327977375623 26.531488973152573 26.634845852408912 23.37038540068228 24 23.513447533535608 26.22768387912914 26.958951970603433 23.29991661673182 25 23.51226332542833 26.277535413998198 26.758810698388142 23.45139056218533 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 152.0 1 152.0 2 152.0 3 152.0 4 152.0 5 152.0 6 285.5 7 419.0 8 419.0 9 419.0 10 710.5 11 1002.0 12 1002.0 13 1002.0 14 1515.5 15 2029.0 16 2029.0 17 2029.0 18 3633.5 19 5238.0 20 5238.0 21 5238.0 22 8312.5 23 11387.0 24 11387.0 25 11387.0 26 16347.5 27 21308.0 28 21308.0 29 21308.0 30 28499.5 31 35691.0 32 35691.0 33 35691.0 34 42105.5 35 48520.0 36 48520.0 37 48520.0 38 55047.5 39 61575.0 40 61575.0 41 61575.0 42 67595.0 43 73615.0 44 73615.0 45 73615.0 46 78314.5 47 83014.0 48 83014.0 49 83014.0 50 83718.5 51 84423.0 52 84423.0 53 84423.0 54 78034.0 55 71645.0 56 71645.0 57 71645.0 58 62950.0 59 54255.0 60 54255.0 61 54255.0 62 45873.0 63 37491.0 64 37491.0 65 37491.0 66 29785.0 67 22079.0 68 22079.0 69 22079.0 70 16246.0 71 10413.0 72 10413.0 73 10413.0 74 7344.5 75 4276.0 76 4276.0 77 4276.0 78 2892.5 79 1509.0 80 1509.0 81 1509.0 82 982.0 83 455.0 84 455.0 85 455.0 86 300.0 87 145.0 88 145.0 89 145.0 90 87.5 91 30.0 92 30.0 93 30.0 94 16.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08052946538263386 2 0.015852256965085406 3 0.016169302104387114 4 0.03154599136051996 5 0.015535211825783697 6 0.370308722704395 7 0.16930210438711213 8 0.1772282328696548 9 0.18087425197162446 10 0.009669876748702096 11 0.0017437482661593943 12 0.0023778385447628106 13 0.0 14 0.0668965243926604 15 4.7556770895256217E-4 16 0.0019022708358102487 17 0.0033289739626679348 18 0.0020607934054611026 19 7.926128482542701E-4 20 4.7556770895256217E-4 21 1.5852256965085405E-4 22 0.0 23 1.5852256965085405E-4 24 4.7556770895256217E-4 25 1.5852256965085405E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 630825.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.42341692566775 #Duplication Level Percentage of deduplicated Percentage of total 1 90.93082379024166 74.94829200657516 2 6.303060408957732 10.39039551990386 3 1.3330976093723028 3.2963538017971277 4 0.5030127778226915 1.6584012762167193 5 0.2737432188770373 1.1281425730038181 6 0.15662809888575627 0.7745893858041243 7 0.0994667552000795 0.5738872883869638 8 0.06869754552135213 0.4529829149021157 9 0.05162533420626784 0.3829622800689136 >10 0.25703152415210045 3.667703056432745 >50 0.012759867235505346 0.6861605530116286 >100 0.009279756487199297 1.451976769721443 >500 5.799847802249131E-4 0.34705941656840367 >1k 1.93328260074971E-4 0.24109315760697642 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1513 0.23984464788174217 No Hit GGTATCAACGCAGAGTACTTTTTTT 884 0.14013395157135497 No Hit TATCAACGCAGAGTACTTTTTTTTT 689 0.10922205048943844 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.5852256965085405E-4 0.0 0.0 0.0 0.0 8 1.5852256965085405E-4 0.0 0.0 0.0 0.0 9 1.5852256965085405E-4 0.0 0.0 0.0 0.0 10 1.5852256965085405E-4 0.0 0.0 0.0 0.0 11 1.5852256965085405E-4 0.0 0.0 0.0 0.0 12 1.5852256965085405E-4 0.0 0.0 0.0 0.0 13 1.5852256965085405E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTTAC 55 1.0867929E-5 15.603938 1 TTAGGCC 50 0.0014533903 13.353231 4 GTGTTAC 50 0.0014560154 13.350037 1 TCCAACG 50 0.0015284485 13.264323 18 GTGCTAG 100 1.3684803E-7 12.396462 1 ATAGGAC 85 3.7575883E-6 12.344309 3 GCTAGGC 70 1.0482579E-4 12.264151 3 GTCATAG 70 1.05138744E-4 12.260237 1 TTTAGGA 110 3.583773E-8 12.14124 2 TAGGACC 165 0.0 12.139302 4 TGTCCAA 65 8.1400585E-4 11.670194 10 TAGGACG 180 0.0 11.657583 4 TTAGGAC 135 4.4092303E-9 11.305225 3 CTTACAC 110 4.6955392E-7 11.2731085 3 TAGGCTG 110 4.700396E-7 11.272209 5 TGGCGAG 245 0.0 11.214734 18 TTAGGAG 60 0.0056937 11.128581 3 CTAGACT 60 0.0056970846 11.127693 4 TAAGGGT 60 0.0056970846 11.127693 4 TAGGATC 60 0.0056970846 11.127693 4 >>END_MODULE