FastQCFastQC Report
Thu 26 May 2016
SRR1926606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728609
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA2050.018.0973991
GTATAAG552.4713727E-416.8634851
TGTAGGA4150.015.3886822
TAGGACC1750.015.2509044
CCTTTAA1056.085975E-815.1427211
AAGTAAG550.004795232314.4544151
GTCTTAG658.798037E-414.2691031
GACAGGC509.0035814E-414.2269017
GTCCTAC4600.014.1140031
GACAGTC652.8873465E-514.0705627
GTATAGA852.9651346E-514.0292851
TCGCCAC400.00653628513.79612210
GTGTTAG802.790042E-413.2498811
CTGTAGG4100.013.2498811
GACCTAC600.00853277113.2498811
TCCTAAA2800.013.1025992
ATAGCCC755.816404E-513.0599863
GTAGGAC4000.013.0599863
GTATAGC1051.530785E-512.6189341
TAACACA600.002452929712.4549054
Produced by FastQC (version 0.11.5)