FastQCFastQC Report
Thu 26 May 2016
SRR1926606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728609
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15010.2060089842425773No Hit
CTGTAGGACGTGGAATATGGCAAGA13490.18514731495218972No Hit
GTCCTACAGTGGACATTTCTAAATT13330.18295134976372787No Hit
CTTTAGGACGTGAAATATGGCGAGG11780.1616779370005037No Hit
GTCCTAAAGTGTGTATTTCTCATTT11210.15385481101660836No Hit
GGTATCAACGCAGAGTACTTTTTTT9600.131757911307711No Hit
GTCCTACAGTGTGCATTTCTCATTT9150.12558175921516204No Hit
CTGTAGGACCTGGAATATGGCGAGA7830.10746504641035179No Hit
TATCAACGCAGAGTACTTTTTTTTT7550.10362210733054354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3250.015.2569024
GTAGGAC8500.013.4629023
TGTAGGA8700.013.3726352
CTCCTAG853.7916216E-612.33503151
CTGTAGG9200.012.3289381
TATAGGA1404.9112714E-1112.2608572
TAAGCTG550.002981114412.1361725
GTATAAA802.7657568E-511.9145171
TAGGACA3050.011.5677334
CCAACGA752.1201238E-411.37121919
GATATAC1950.011.2424161
TAATCTG855.1150735E-511.218315
TCCAACG1105.118254E-711.19892718
TCTATAC600.00570654911.1255933
GGGCTAT600.005709484711.1248256
AGGACCT6000.011.1248255
GGACCTG5850.010.9210736
GACGTGG4100.010.6564927
GGACGTG6850.010.5796046
AGGACGT7100.010.475695
Produced by FastQC (version 0.11.5)