FastQCFastQC Report
Thu 26 May 2016
SRR1926605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511512
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG258.319804E-426.7113421
TCTAGGG350.005094271619.5570262
GGCCTAT350.005876931419.0795291
CCCTAAG450.001190295817.8075621
GAGTAGA602.2354003E-517.8075621
GTCCTGA858.317511E-817.283811
CCTATTC452.2590211E-417.061783
CCCTAGG552.3226232E-416.9981271
GTGTTAA500.00240890416.0268061
ATTCAGG500.00240890416.0268061
TCTTATA603.7342566E-415.9715712
CTAGAAC555.743149E-515.9538733
TCCTAAA706.777676E-515.6456212
GTTACAC505.040674E-415.3556033
GTCCTAA658.273059E-414.383031
TAGGGGA450.005140444714.3030144
CTAGACC500.00891266613.1619453
TTCATAC500.00891266613.1619453
CTAAGGG600.001999784312.7963363
TGTACTG1151.8879291E-812.70756155
Produced by FastQC (version 0.11.5)