##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926605_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 511512 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.919436885156163 27.0 2.0 31.0 2.0 31.0 2 16.094140508922568 16.0 2.0 30.0 2.0 31.0 3 19.63034689313252 22.0 15.0 31.0 2.0 31.0 4 21.667526861539905 27.0 2.0 35.0 2.0 37.0 5 22.506627410500634 29.0 2.0 35.0 2.0 37.0 6 23.18525860585871 30.0 2.0 35.0 2.0 37.0 7 22.858984735450978 30.0 2.0 35.0 2.0 37.0 8 23.132522795164142 30.0 2.0 35.0 2.0 37.0 9 25.754177810100252 31.0 17.0 37.0 2.0 39.0 10 26.488969955739062 31.0 20.0 37.0 2.0 39.0 11 23.618704937518572 30.0 7.0 37.0 2.0 39.0 12 24.75402141103239 31.0 10.0 37.0 2.0 39.0 13 21.42670748682338 27.0 2.0 35.0 2.0 39.0 14 24.73523592799387 30.0 12.0 38.0 2.0 40.0 15 25.813673970503135 31.0 16.0 38.0 2.0 40.0 16 26.1677008555029 31.0 12.0 38.0 2.0 40.0 17 25.470735779414756 31.0 10.0 38.0 2.0 40.0 18 25.55206915966781 31.0 10.0 38.0 2.0 40.0 19 25.95203436087521 31.0 10.0 38.0 2.0 40.0 20 26.209547381097607 31.0 10.0 39.0 2.0 40.0 21 24.066987675753452 30.0 2.0 37.0 2.0 40.0 22 24.961437854830386 31.0 2.0 38.0 2.0 40.0 23 24.951934656469447 31.0 2.0 38.0 2.0 40.0 24 24.03658956192621 31.0 2.0 38.0 2.0 40.0 25 23.820692378673424 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.748007346721877 1101 2 10.328680273224764 1101 3 6.754972910085556 1101 4 8.304784165300799 1101 5 8.216716096909387 1101 6 7.522871436708165 1101 7 7.249482812421867 1101 8 5.683620071456996 1101 9 5.591424500827706 1101 10 5.203580836280214 1101 11 7.951586605490476 1101 12 5.467183153484722 1101 13 10.035744500594546 1101 14 7.339915162634316 1101 15 6.572976251283116 1101 16 6.092441545249464 1101 17 2.04576586858591 1101 18 4.778571441860123 1101 19 5.469443705278216 1101 20 3.4971436043199944 1101 21 0.4126710454296294 1101 22 4.186710657033483 1101 23 5.274717883716143 1101 24 6.853046872507463 1101 25 7.28317218206201 1102 1 5.605543231889342 1102 2 10.145244713823361 1102 3 6.621697688666437 1102 4 7.924671165198998 1102 5 7.746227193315779 1102 6 6.42898973050843 1102 7 6.81381524650514 1102 8 6.897220804430628 1102 9 5.834546510397086 1102 10 5.250543322282454 1102 11 7.8902509646475565 1102 12 5.12237123248299 1102 13 9.53385480294617 1102 14 7.055158723664551 1102 15 4.958244244767794 1102 16 5.338266395091672 1102 17 6.134304247899767 1102 18 6.185264916358747 1102 19 4.700088620273636 1102 20 5.153465501907288 1102 21 4.034884218594648 1102 22 5.259641533547786 1102 23 5.70914728410299 1102 24 7.212048704941548 1102 25 6.97166729557112 1103 1 5.274400391815263 1103 2 9.548901354640511 1103 3 6.517741200251692 1103 4 8.082882400777013 1103 5 7.373081539072217 1103 6 7.182101650308411 1103 7 7.0297106010917645 1103 8 6.544769591630352 1103 9 5.541577993759294 1103 10 4.8377323686298155 1103 11 5.703985738706862 1103 12 4.824913263896445 1103 13 8.611297849062197 1103 14 7.004344788059971 1103 15 5.919177623690505 1103 16 5.542063617275055 1103 17 2.8934970108828857 1103 18 1.7570205183228182 1103 19 4.0380253789961 1103 20 4.558009356048402 1103 21 6.959570230240278 1103 22 2.586442366867349 1103 23 5.0182737603026695 1103 24 6.256573340364238 1103 25 2.568409773641825 1104 1 5.62555971605277 1104 2 10.238307586627245 1104 3 6.88023475674002 1104 4 7.398815078459229 1104 5 7.44223216361673 1104 6 7.262184025685183 1104 7 7.137994608232319 1104 8 7.049859648434829 1104 9 6.164347522995694 1104 10 5.013004773640393 1104 11 7.914775541833773 1104 12 4.862309755071056 1104 13 9.287282531914457 1104 14 7.146486751001206 1104 15 6.164752232572521 1104 16 5.875035098228924 1104 17 6.290638250119365 1104 18 3.5371742484495066 1104 19 4.51629646102359 1104 20 3.832398841253081 1104 21 2.6514345190924296 1104 22 5.051689302549043 1104 23 5.498451867852811 1104 24 6.791632392133252 1104 25 5.860488126743725 1105 1 3.544149590305068 1105 2 9.403070548304017 1105 3 6.429877041387325 1105 4 5.895943793074601 1105 5 2.6237145118802445 1105 6 5.239447773756943 1105 7 6.121728185159363 1105 8 3.6521853644317375 1105 9 4.410060097514485 1105 10 3.8044666630518655 1105 11 7.190926006247199 1105 12 4.662281067381848 1105 13 5.211855299244714 1105 14 4.026374667696185 1105 15 4.729366219582534 1105 16 4.6639301943955935 1105 17 5.3367222547194615 1105 18 2.9744350270937865 1105 19 2.9759499942648233 1105 20 1.8903995609617752 1105 21 3.192607644263866 1105 22 4.417838584224402 1105 23 2.003238182315183 1105 24 4.810933670748593 1105 25 5.551618597850943 1106 1 6.504690217324264 1106 2 11.053486076572463 1106 3 7.689889468169149 1106 4 8.901942629769074 1106 5 8.381506364524903 1106 6 7.74129380816251 1106 7 7.3920168908021395 1106 8 7.121507211879969 1106 9 6.194843614789821 1106 10 5.615446878176574 1106 11 8.147086254683622 1106 12 5.427851440544959 1106 13 4.080603927275913 1106 14 5.600981866052177 1106 15 5.836583798642042 1106 16 5.863290748416748 1106 17 6.286097381064611 1106 18 6.342373185871349 1106 19 6.276337059994063 1106 20 5.818878594798093 1106 21 5.419386490954334 1106 22 6.006355840956505 1106 23 6.68923126449193 1106 24 7.308045870202161 1106 25 6.071426416801909 1107 1 3.974837596118501 1107 2 -13.656546999502508 1107 3 -5.31917230310833 1107 4 1.759550858784305 1107 5 4.657192766257737 1107 6 4.971725016756434 1107 7 4.752952241223152 1107 8 5.433780972502852 1107 9 3.752979207183298 1107 10 3.4541339336253465 1107 11 5.922523854082591 1107 12 3.2170121961234983 1107 13 4.614837420948529 1107 14 4.3959992334307785 1107 15 4.005588882015218 1107 16 4.050194964396688 1107 17 4.4245570994789105 1107 18 -0.8416417520256374 1107 19 2.116668162237186 1107 20 2.507039018640185 1107 21 -0.8454745700168722 1107 22 2.3923824188999347 1107 23 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0.5771277310316023 1109 12 1.1245068257474173 1109 13 2.7498124448454178 1109 14 3.0613001800559196 1109 15 3.054898698754343 1109 16 2.7994986141967786 1109 17 3.212813962768152 1109 18 3.1917432294221513 1109 19 1.7383303064818918 1109 20 0.3649347562574867 1109 21 0.00785634793200174 1109 22 2.120381863875423 1109 23 2.5565043898928934 1109 24 3.782629281967921 1109 25 4.004900352265789 1110 1 3.181304521026039 1110 2 8.904570579077236 1110 3 6.480262623282719 1110 4 7.592798770020874 1110 5 8.323913948533896 1110 6 4.430417208747535 1110 7 6.631328774957584 1110 8 7.256230929748877 1110 9 5.660353752600383 1110 10 5.297367215791272 1110 11 3.1539889761117053 1110 12 5.810181089774275 1110 13 10.339222381851823 1110 14 7.980645663216386 1110 15 7.1060710232166535 1110 16 6.660010864754177 1110 17 7.404469170522624 1110 18 7.390578003401028 1110 19 6.195486143631481 1110 20 6.600662495207917 1110 21 4.19360024428665 1110 22 6.476074610891036 1110 23 6.70367332873678 1110 24 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16 5.6700133155843915 2205 17 6.686375916432802 2205 18 6.4292692090052554 2205 19 4.985292987574869 2205 20 5.608905964766564 2205 21 -0.6616909960376773 2205 22 4.124469827687392 2205 23 5.172006559952187 2205 24 7.075873826255471 2205 25 7.471130714757383 2206 1 3.5512778566997056 2206 2 7.747000672337846 2206 3 5.536541646227462 2206 4 0.791591260644477 2206 5 4.908151795357281 2206 6 5.278082079723685 2206 7 5.451672391135833 2206 8 5.089163308707551 2206 9 3.8207759420693925 2206 10 2.9032759582314327 2206 11 3.043604343849676 2206 12 4.19614656811887 2206 13 7.172523879751974 2206 14 -0.37962474867388707 2206 15 1.621839887646754 2206 16 2.386870591812656 2206 17 3.3669687510692796 2206 18 -1.4428254539270178 2206 19 0.12714880505648907 2206 20 1.589937395894271 2206 21 2.476972597536058 2206 22 -2.788233910594009 2206 23 -3.488475020940175 2206 24 0.20908900111056639 2206 25 1.8236100978048029 2207 1 1.64670459158825 2207 2 -13.656546999502508 2207 3 -6.1031441572965495 2207 4 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17 5.836164705107308 2208 18 0.7325548770799983 2208 19 3.5276422854399208 2208 20 4.323775432361799 2208 21 7.158184750813181 2208 22 6.090043333034274 2208 23 1.6229332258577323 2208 24 3.2796047506380113 2208 25 4.604605331342231 2209 1 3.2402571644871614 2209 2 5.094884844712842 2209 3 5.357502067640489 2209 4 1.6879092303846264 2209 5 5.167110504906887 2209 6 5.694151245101374 2209 7 5.993280323616283 2209 8 4.650734514860098 2209 9 4.279171819373996 2209 10 3.1614130241381737 2209 11 -0.6807678670701804 2209 12 2.9034699909313524 2209 13 2.255174626596837 2209 14 3.40230061509709 2209 15 4.131940897747764 2209 16 3.5439658085005306 2209 17 0.9816674097709566 2209 18 3.010993601601868 2209 19 3.9397264294269227 2209 20 4.124452778536384 2209 21 6.065904410003199 2209 22 4.697426058470533 2209 23 0.09773152528648765 2209 24 -2.813101726888558 2209 25 0.16741018060413282 2210 1 -2.290530083200075 2210 2 -13.656546999502508 2210 3 -7.590782558750323 2210 4 -2.4397634877716428 2210 5 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-7.644120359929925 2211 18 -5.570455690341802 2211 19 -8.522111261752375 2211 20 -6.446121988588882 2211 21 -4.313736910467032 2211 22 -5.742399709413387 2211 23 -7.998571490405574 2211 24 -21.640134945674355 2211 25 -21.42250963611981 2212 1 -2.0749305156564155 2212 2 -13.656546999502508 2212 3 -8.265417364501468 2212 4 -19.190670380153747 2212 5 -20.025329357636068 2212 6 -20.695310381473654 2212 7 -10.456477968674294 2212 8 -10.03474772200105 2212 9 -6.151266397323479 2212 10 -6.661265734116899 2212 11 -6.288266802232968 2212 12 -3.7937688758337345 2212 13 -7.215834921788124 2212 14 -4.94379422364727 2212 15 -4.908572539877589 2212 16 -5.073304817292929 2212 17 -5.2162144946128635 2212 18 -4.395517826272467 2212 19 -4.897707044092748 2212 20 -4.995652588656707 2212 21 -2.8341290858470387 2212 22 -4.093696300629791 2212 23 -4.302374338141419 2212 24 -3.280327484783861 2212 25 -3.7414025362401446 2213 1 -18.499719926005394 2213 2 -13.656546999502508 2213 3 -8.40556569514483 2213 4 -5.581704004064084 2213 5 -20.025329357636068 2213 6 -10.6753851012744 2213 7 -20.369835369396316 2213 8 -10.412021273499418 2213 9 -8.3532361467439 2213 10 -7.0055567726861625 2213 11 -6.288139421906784 2213 12 -5.103598291098091 2213 13 -18.901755499405454 2213 14 -12.060972134999556 2213 15 -6.662718020198827 2213 16 -6.03210702262102 2213 17 -4.613683072883578 2213 18 -4.514143377567027 2213 19 -5.302505236191273 2213 20 -5.421847678369922 2213 21 -3.6396328150934103 2213 22 -4.673314249566765 2213 23 -4.463264681663681 2213 24 -3.5890764151637704 2213 25 -4.550778627402497 2214 1 -18.499719926005394 2214 2 -13.656546999502508 2214 3 -11.821163453550806 2214 4 -19.190670380153747 2214 5 -20.025329357636068 2214 6 -20.695310381473654 2214 7 -15.857335369396317 2214 8 -14.454763766926844 2214 9 -13.188626004222797 2214 10 -13.014474719275341 2214 11 -13.838943697539827 2214 12 -11.585089573788006 2214 13 -18.901755499405454 2214 14 -16.158695948476794 2214 15 -23.423614657807793 2214 16 -17.636346333538413 2214 17 -23.103097767777726 2214 18 -17.057792194503513 2214 19 -14.28232397148946 2214 20 -12.852351345174956 2214 21 -10.409424914166332 2214 22 -11.399652979330153 2214 23 -11.335888176889917 2214 24 -10.260968279007688 2214 25 -9.975287413897586 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 97809.0 3 0.0 4 409.0 5 1729.0 6 2445.0 7 2329.0 8 1909.0 9 1871.0 10 1738.0 11 1633.0 12 1577.0 13 1696.0 14 2532.0 15 5393.0 16 7650.0 17 8071.0 18 8498.0 19 9955.0 20 11610.0 21 13541.0 22 13944.0 23 14965.0 24 15741.0 25 15861.0 26 13199.0 27 10897.0 28 11608.0 29 13355.0 30 12714.0 31 14924.0 32 19094.0 33 23813.0 34 28389.0 35 32672.0 36 31683.0 37 32632.0 38 23626.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17702547826853 18.248688335966833 11.434364806488585 26.13992137927605 2 15.20192078908092 20.496760058958582 39.152454908116695 25.148864243843803 3 18.926654071344394 24.41164672407307 28.499514685189688 28.162184519392845 4 11.66196004602029 17.207586981532767 37.61044642410518 33.52000654834177 5 12.847398477157359 37.1673313995649 36.546863669325596 13.43840645395214 6 32.59036824325102 36.86600606561643 17.835307230150306 12.708318460982243 7 28.869949481314134 30.934325708373922 22.266450970524648 17.9292738397873 8 25.381999440976426 37.04293470435276 20.160634576454946 17.414431278215865 9 26.46927004742138 15.532800601231822 19.541795592923783 38.456133758423015 10 14.046615595042653 29.698433904994076 34.58630197900889 21.668648520954388 11 33.71856120611874 22.756925993838017 23.80783095710312 19.716681842940122 12 22.73186762901179 25.007383988185616 31.675229319633086 20.5855190631695 13 28.00948322513992 21.24636003926355 25.187005545156598 25.557151190439935 14 20.928466491591834 21.537782566602324 27.83371734493254 29.700033596873304 15 23.053679775847126 30.001814186941882 23.61426354088976 23.330242496321233 16 22.634748891501207 28.33562863324468 26.40152760106545 22.628094874188665 17 21.979561114122824 27.8353361775535 27.91131192174297 22.273790786580708 18 21.695935934663527 27.75965965289998 28.871706462543152 21.672697949893344 19 22.66123492672313 27.451727793593882 27.218853855225074 22.66818342445792 20 22.947039863227488 27.494490671393056 27.824658264777156 21.7338112006023 21 22.39055477953016 26.266032445382514 26.85799965950604 24.48541311558129 22 22.477038686334538 27.237087988549163 27.982386386227187 22.30348693888911 23 22.51596526490695 27.246145927316213 27.641485616093846 22.59640319168299 24 22.06116491258308 27.372106300696963 27.777143411144984 22.78958537557497 25 22.700716926551724 27.042594792643232 27.630736831392106 22.625951449412934 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 536.0 1 536.0 2 1163.5 3 1791.0 4 1791.0 5 1791.0 6 2549.0 7 3307.0 8 3307.0 9 3307.0 10 3874.0 11 4441.0 12 4441.0 13 4441.0 14 5413.0 15 6385.0 16 6385.0 17 6385.0 18 8681.0 19 10977.0 20 10977.0 21 10977.0 22 14947.5 23 18918.0 24 18918.0 25 18918.0 26 24487.0 27 30056.0 28 30056.0 29 30056.0 30 35904.5 31 41753.0 32 41753.0 33 41753.0 34 46682.5 35 51612.0 36 51612.0 37 51612.0 38 55251.5 39 58891.0 40 58891.0 41 58891.0 42 60561.0 43 62231.0 44 62231.0 45 62231.0 46 61998.0 47 61765.0 48 61765.0 49 61765.0 50 58240.0 51 54715.0 52 54715.0 53 54715.0 54 48175.5 55 41636.0 56 41636.0 57 41636.0 58 35057.0 59 28478.0 60 28478.0 61 28478.0 62 23104.0 63 17730.0 64 17730.0 65 17730.0 66 13656.5 67 9583.0 68 9583.0 69 9583.0 70 6904.5 71 4226.0 72 4226.0 73 4226.0 74 2960.5 75 1695.0 76 1695.0 77 1695.0 78 1120.0 79 545.0 80 545.0 81 545.0 82 365.5 83 186.0 84 186.0 85 186.0 86 115.5 87 45.0 88 45.0 89 45.0 90 27.0 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.515811945760802 2 36.733253569808724 3 5.939450100877399 4 14.018439450100878 5 13.730274167565963 6 15.104435477564554 7 9.48462597162921 8 7.675284255305838 9 0.10967484633791583 10 1.520003440779493 11 5.962323464552152 12 6.010025180249926 13 16.549562864605328 14 1.6595896088459312 15 3.014983030701137 16 3.0441123570903517 17 2.9913276716870767 18 1.569073648321056 19 1.5260639046591282 20 0.28699228952595446 21 4.689039553324262 22 1.660176105350412 23 0.10928384866826193 24 9.258238320899608 25 0.1135848230344547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 511512.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.70527513316921 #Duplication Level Percentage of deduplicated Percentage of total 1 97.57997305035613 93.38918168271569 2 1.8486146191955173 3.5384434149061166 3 0.29248474857914475 0.8397700000506859 4 0.09727692177105776 0.3723965824882744 5 0.052092746472493914 0.249277531679623 6 0.032971045842407974 0.1893301808265594 7 0.02297241721836315 0.15390070572501793 8 0.016618616913877494 0.1272391443260308 9 0.01025765748532737 0.08835407386695616 >10 0.04342267008567032 0.7165498884600617 >50 0.0018649724987053543 0.13822879936905003 >100 0.0014505335812972867 0.1973279955859289 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 1.9549883482694442E-4 0.0 0.0 0.0 0.0 11 1.9549883482694442E-4 0.0 0.0 0.0 0.0 12 1.9549883482694442E-4 0.0 0.0 0.0 0.0 13 1.9549883482694442E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 25 8.319804E-4 26.711342 1 TCTAGGG 35 0.0050942716 19.557026 2 GGCCTAT 35 0.0058769314 19.079529 1 CCCTAAG 45 0.0011902958 17.807562 1 GAGTAGA 60 2.2354003E-5 17.807562 1 GTCCTGA 85 8.317511E-8 17.28381 1 CCTATTC 45 2.2590211E-4 17.06178 3 CCCTAGG 55 2.3226232E-4 16.998127 1 GTGTTAA 50 0.002408904 16.026806 1 ATTCAGG 50 0.002408904 16.026806 1 TCTTATA 60 3.7342566E-4 15.971571 2 CTAGAAC 55 5.743149E-5 15.953873 3 TCCTAAA 70 6.777676E-5 15.645621 2 GTTACAC 50 5.040674E-4 15.355603 3 GTCCTAA 65 8.273059E-4 14.38303 1 TAGGGGA 45 0.0051404447 14.303014 4 CTAGACC 50 0.008912666 13.161945 3 TTCATAC 50 0.008912666 13.161945 3 CTAAGGG 60 0.0019997843 12.796336 3 TGTACTG 115 1.8879291E-8 12.7075615 5 >>END_MODULE