FastQCFastQC Report
Thu 26 May 2016
SRR1926605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511512
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9620.18806987910352055No Hit
GGTATCAACGCAGAGTACTTTTTTT5350.10459187663241526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACT350.00211201616.3490524
TGGCGAG1700.014.49039218
CCTAGCC400.005128519614.3096463
ATTATGC400.005128519614.3096463
CCTAACC551.8879697E-413.87601953
TAGGACC1600.013.7093624
CTCCTAG852.5659938E-713.4626031
AGTACCG500.001453964613.35172755
CTAGACA603.9520473E-412.7159294
GGCGAGG604.177381E-412.63264919
GTAGGAC2450.012.4600983
TGTAGGA2400.012.324622
TTAGGAC1251.3096724E-912.2108973
ATGGCGA1950.012.14677717
TAACATA550.00297577312.1379334
GGCGAGA1451.0186341E-1011.76143219
CTATAAG657.756962E-411.7366281
ACTGTCC1001.919845E-611.4005888
CTGTAGG2350.011.3620551
TATGGCG2100.011.27915116
Produced by FastQC (version 0.11.5)