##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926605_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 511512 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.39878047826835 31.0 28.0 31.0 22.0 34.0 2 28.872327530927915 31.0 28.0 33.0 23.0 34.0 3 29.233095215752513 31.0 29.0 33.0 25.0 34.0 4 33.02450968892225 35.0 33.0 37.0 27.0 37.0 5 32.689283926867795 35.0 33.0 37.0 26.0 37.0 6 32.77982725722955 35.0 33.0 37.0 26.0 37.0 7 32.57864722626253 35.0 33.0 37.0 25.0 37.0 8 32.540771282003156 35.0 33.0 37.0 25.0 37.0 9 33.7717492453745 37.0 34.0 39.0 23.0 39.0 10 33.68392334881684 37.0 33.0 39.0 23.0 39.0 11 33.92458241448881 37.0 34.0 39.0 25.0 39.0 12 33.71947481193012 37.0 34.0 39.0 23.0 39.0 13 33.86354963324418 37.0 34.0 39.0 25.0 39.0 14 34.52163976602699 38.0 34.0 40.0 22.0 41.0 15 34.6431892115923 38.0 34.0 40.0 24.0 41.0 16 34.60381965623485 38.0 34.0 40.0 24.0 41.0 17 34.513186396409075 38.0 34.0 40.0 22.0 41.0 18 34.47160965920643 38.0 34.0 40.0 22.0 41.0 19 34.52165345094543 39.0 34.0 40.0 22.0 41.0 20 34.505892334881686 39.0 34.0 40.0 21.0 41.0 21 34.42790980465757 39.0 34.0 40.0 19.0 41.0 22 34.362869297298985 39.0 34.0 40.0 19.0 41.0 23 34.01308669200331 39.0 34.0 40.0 17.0 41.0 24 34.0971551009556 39.0 34.0 40.0 18.0 41.0 25 33.90055169771188 39.0 34.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7887637976255952 1101 2 0.7427259351500695 1101 3 0.7833050996263999 1101 4 0.7314675968876756 1101 5 0.9999667637771665 1101 6 0.2856454675881963 1101 7 0.5996543647699397 1101 8 0.74666461495395 1101 9 1.2458902927477382 1101 10 1.0156710188817755 1101 11 0.8563453615549506 1101 12 0.9485298939707505 1101 13 0.8242478259355508 1101 14 1.0726675405884833 1101 15 0.8313823909648477 1101 16 0.9540552924482668 1101 17 0.9481636243566456 1101 18 1.0422286004553882 1101 19 0.8809430886370606 1101 20 0.819414994075153 1101 21 0.7910691068364102 1101 22 0.898394826075517 1101 23 1.4028884212491874 1101 24 1.2005288373956233 1101 25 1.2403040048943481 1102 1 0.7181056483840216 1102 2 0.6636907625958592 1102 3 0.8085863273572329 1102 4 0.46806537760639344 1102 5 0.6624464033984623 1102 6 0.06416399553281593 1102 7 0.4544810470462295 1102 8 0.46315270413241194 1102 9 0.7499140125889312 1102 10 1.21795392634386 1102 11 0.6095584838190291 1102 12 0.8074248344166435 1102 13 0.8251945835452474 1102 14 1.1457676627507567 1102 15 0.7513202918098045 1102 16 0.6344787883252891 1102 17 0.8159662304851167 1102 18 0.7558878695177924 1102 19 0.8345634184751987 1102 20 0.6231498510434932 1102 21 0.7447708781893567 1102 22 0.8186457677430283 1102 23 1.1303545721195931 1102 24 1.1333904886223394 1102 25 1.2206715097299394 1103 1 0.11548188657410563 1103 2 0.23355118538224318 1103 3 0.17836107940144785 1103 4 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2207 9 0.8498449929850764 2207 10 0.8265187126182312 2207 11 0.6875353832245423 2207 12 0.39050956583123764 2207 13 0.44442118265828867 2207 14 -4.363787675591475 2207 15 -1.5924799555422169 2207 16 -0.40972553512370524 2207 17 -0.18621621260548693 2207 18 0.016759092664656805 2207 19 0.27896831823153434 2207 20 0.24024230807783908 2207 21 0.4561049648490041 2207 22 0.5270322215971319 2207 23 0.7589197905609169 2207 24 0.49198482748952443 2207 25 0.5751043350976346 2208 1 0.20223747145718818 2208 2 0.6955631467454175 2208 3 0.6692718714662718 2208 4 0.46923135157116747 2208 5 0.4162825791981888 2208 6 0.1690190625398813 2208 7 0.3487891508081944 2208 8 0.20408179105334057 2208 9 0.6770527853527497 2208 10 0.6132701117628656 2208 11 0.3299822181907217 2208 12 0.34129759264951076 2208 13 0.3260423437821345 2208 14 0.5610450795354396 2208 15 0.535553165959918 2208 16 0.5770290648797385 2208 17 0.49228214141442095 2208 18 0.6079306316129518 2208 19 0.17023760648364572 2208 20 0.11383177577501868 2208 21 0.2896122935845895 2208 22 0.6617797600135944 2208 23 0.8257438738226455 2208 24 0.7964665222703999 2208 25 0.9282452393648697 2209 1 -0.09195837252956451 2209 2 -0.09108771683368388 2209 3 0.6432017465075823 2209 4 0.8837741677509428 2209 5 0.6500539761066477 2209 6 0.5621879543110921 2209 7 0.8561067754749274 2209 8 0.7749695717382892 2209 9 0.9542652797309756 2209 10 0.6634399452589506 2209 11 0.8438037079050531 2209 12 0.5743161066126348 2209 13 0.5520724640694681 2209 14 0.4468019256764748 2209 15 0.5713126211012636 2209 16 0.6968231774841911 2209 17 0.6362741106630097 2209 18 0.7971775747342633 2209 19 0.7026823888557487 2209 20 0.47549512528414795 2209 21 0.7380609323076186 2209 22 0.8069780813082588 2209 23 0.8912670529267857 2209 24 0.8277807378433977 2209 25 0.9187534477767798 2210 1 -0.1706651349895516 2210 2 -0.2634450552863363 2210 3 0.4539836200177376 2210 4 0.7865846684497271 2210 5 0.7790106793537319 2210 6 0.8832174693470165 2210 7 1.0406280374092702 2210 8 1.0343899639701135 2210 9 1.213898976929336 2210 10 1.1415339407241447 2210 11 0.9291921791871403 2210 12 0.7195615527593588 2210 13 0.8741214106338049 2210 14 1.2097937910007062 2210 15 0.9419078389147231 2210 16 1.0036540612731528 2210 17 1.0432031978424092 2210 18 0.6942814750294275 2210 19 0.9220651618480105 2210 20 1.0531637574037234 2210 21 1.231795445189718 2210 22 1.162039434188081 2210 23 1.253949210520375 2210 24 1.1897010919850501 2210 25 1.1546548898014635 2211 1 -1.1098174368355416 2211 2 -0.17078746880626028 2211 3 0.3995614502911913 2211 4 0.6930068899370738 2211 5 1.0656231792998128 2211 6 0.9031827608537881 2211 7 0.9206048025604474 2211 8 0.9422988707100899 2211 9 1.1599455300193853 2211 10 1.4105391412469288 2211 11 1.2251230612630906 2211 12 1.417044108650245 2211 13 1.2362140998518854 2211 14 1.5639455105376783 2211 15 1.2945052965966752 2211 16 1.2431699827520148 2211 17 1.1956747042398987 2211 18 1.4180987766525561 2211 19 1.3295175670718251 2211 20 1.3447352297253516 2211 21 1.4213104320985437 2211 22 1.1833505065424887 2211 23 1.5718755577749661 2211 24 1.3953726380658225 2211 25 1.608431774865167 2212 1 0.9655469723329588 2212 2 0.05387487946688552 2212 3 0.38959680731702306 2212 4 0.5506558695055475 2212 5 0.6072868053481102 2212 6 0.5671751142351695 2212 7 0.5986684223128762 2212 8 0.7598674921008737 2212 9 0.7932784340889469 2212 10 0.3424049648395524 2212 11 0.4789181107071272 2212 12 0.7501899968041386 2212 13 0.4280220300707711 2212 14 0.6109770800261813 2212 15 0.28350469705827663 2212 16 -0.12650482132484342 2212 17 0.47288466144230057 2212 18 0.4591879046890597 2212 19 0.4665614564305187 2212 20 0.5562449360946289 2212 21 0.4313423263966385 2212 22 0.5618752986715094 2212 23 0.6573791771839836 2212 24 0.6266857130109855 2212 25 0.5134743363679277 2213 1 0.5900402608883617 2213 2 0.3415895715137083 2213 3 -0.3155896699875491 2213 4 -1.0897030133240335 2213 5 -1.6540961253884632 2213 6 -0.44332402182649844 2213 7 -0.13171238491872828 2213 8 -0.07673202265501544 2213 9 -0.9365194208263574 2213 10 -0.5465643484905769 2213 11 -0.46549461353844634 2213 12 -0.7084750873492993 2213 13 -0.8229501815364273 2213 14 -7.785598337369173 2213 15 -3.6349314321982895 2213 16 -2.1308762144010487 2213 17 -1.3408463382834555 2213 18 -5.492298174139879 2213 19 -2.8735245327826107 2213 20 -4.995855865202554 2213 21 -2.1043698719929793 2213 22 -1.6419227330776565 2213 23 -3.9845337456250327 2213 24 -1.9856479994661456 2213 25 -1.7233946252426335 2214 1 0.39267793903973924 2214 2 0.2569936119177463 2214 3 -1.7834878296665302 2214 4 -2.0651233122032338 2214 5 -0.5799574786470743 2214 6 -0.6634706940279642 2214 7 -0.5014819988664243 2214 8 -0.5323757890864513 2214 9 -5.581129909272462 2214 10 -1.865010799300041 2214 11 -1.045801103091513 2214 12 -1.3618236413827844 2214 13 -1.576636012448283 2214 14 -2.583266802845859 2214 15 -2.0774559928735385 2214 16 -1.6854649095719338 2214 17 -1.9071394059463884 2214 18 -1.5339077631809772 2214 19 -4.657440749746776 2214 20 -2.633110258450099 2214 21 -4.212339984072678 2214 22 -6.058170830490141 2214 23 -6.414030770670003 2214 24 -5.553132778765992 2214 25 -3.5631050860147404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16161.0 3 7.0 4 329.0 5 2025.0 6 2522.0 7 2065.0 8 1575.0 9 1260.0 10 1107.0 11 1041.0 12 1030.0 13 965.0 14 980.0 15 971.0 16 997.0 17 1047.0 18 1229.0 19 1404.0 20 1859.0 21 2375.0 22 3204.0 23 3895.0 24 4731.0 25 5374.0 26 6168.0 27 6901.0 28 7947.0 29 9603.0 30 11942.0 31 14697.0 32 19103.0 33 24791.0 34 32543.0 35 42250.0 36 57506.0 37 83970.0 38 135938.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.03923732594454 18.807773890310813 8.829181048989911 30.323807734754737 2 14.230484928838777 21.425610218744012 37.84255750194574 26.501347350471477 3 18.022586164751893 24.549694451234416 29.152578831581522 28.27514055243217 4 12.279744513413828 17.196062095913074 36.59145222924954 33.93274116142356 5 13.34350130032655 38.13828435110772 34.85813730666197 13.660077041903756 6 32.24288428026438 37.706249901879204 17.19449110633154 12.856374711524875 7 28.556285317929948 31.634676427020715 21.621039101504024 18.187999153545313 8 25.911041442147546 36.78436367198981 19.692955814637013 17.61163907122563 9 26.447944161292664 15.382098144914927 19.393055824990643 38.776901868801765 10 14.406913879574141 29.85149628007469 33.83890425958333 21.902685580767837 11 34.993802565776875 23.02923546731 23.069900019941272 18.907061946971858 12 22.483416325022436 25.335436962484632 31.27486573723795 20.906280975254987 13 28.688476516679962 21.88159808567541 25.46939270241949 23.960532695225137 14 21.098164118135006 21.569459878233925 27.306590706337158 30.02578529729391 15 23.32884982326629 29.948152898120057 23.08681848040289 23.636178798210768 16 22.854333171715908 28.60296072696561 25.989841562788367 22.552864538530116 17 22.20873216966967 28.368016453953953 26.944718155655657 22.47853322072072 18 22.029345350394433 28.080626399085034 27.702519086208078 22.18750916431246 19 23.15601173020528 27.532551319648096 27.02815249266862 22.283284457478008 20 23.03070943726735 27.46215083362007 27.546412149269607 21.960727579842974 21 23.07355448161529 26.80249925710443 27.157916138819814 22.96603012246047 22 22.705039177966498 27.31822518337791 27.18039850482491 22.796337133830683 23 22.713445627864058 27.16886407357012 27.126831824082327 22.990858474483492 24 22.540820156711867 27.033383381035048 27.656438167628522 22.769358294624563 25 22.61456742865745 27.09423648758287 27.316128098906965 22.97506798485272 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 126.0 1 126.0 2 125.5 3 125.0 4 125.0 5 125.0 6 271.5 7 418.0 8 418.0 9 418.0 10 700.0 11 982.0 12 982.0 13 982.0 14 1571.5 15 2161.0 16 2161.0 17 2161.0 18 3721.0 19 5281.0 20 5281.0 21 5281.0 22 8289.5 23 11298.0 24 11298.0 25 11298.0 26 16044.5 27 20791.0 28 20791.0 29 20791.0 30 26760.5 31 32730.0 32 32730.0 33 32730.0 34 38598.0 35 44466.0 36 44466.0 37 44466.0 38 49585.0 39 54704.0 40 54704.0 41 54704.0 42 58868.5 43 63033.0 44 63033.0 45 63033.0 46 65893.5 47 68754.0 48 68754.0 49 68754.0 50 67373.0 51 65992.0 52 65992.0 53 65992.0 54 59690.5 55 53389.0 56 53389.0 57 53389.0 58 45697.5 59 38006.0 60 38006.0 61 38006.0 62 31427.5 63 24849.0 64 24849.0 65 24849.0 66 19554.0 67 14259.0 68 14259.0 69 14259.0 70 10334.5 71 6410.0 72 6410.0 73 6410.0 74 4442.5 75 2475.0 76 2475.0 77 2475.0 78 1687.5 79 900.0 80 900.0 81 900.0 82 581.0 83 262.0 84 262.0 85 262.0 86 169.0 87 76.0 88 76.0 89 76.0 90 48.5 91 21.0 92 21.0 93 21.0 94 12.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09149345469900999 2 0.026978839206118328 3 0.02678334037129139 4 0.034016797259888334 5 0.019940881152348332 6 0.3784857442249644 7 0.22443266238133222 8 0.2271696460689094 9 0.23166611926992917 10 0.015053410281674721 11 0.001954988348269444 12 0.0029324825224041665 13 0.0 14 0.07194357121631555 15 0.0015639906786155554 16 0.0031279813572311107 17 0.004691972035846667 18 0.0033234801920580554 19 0.0023459860179233334 20 0.0015639906786155554 21 0.0 22 0.0 23 0.0 24 0.0 25 1.9549883482694442E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 511512.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.22608111336466 #Duplication Level Percentage of deduplicated Percentage of total 1 93.08740268524612 81.19649337255717 2 5.20038193865794 9.07217873603708 3 0.8399375278406469 2.197933768007617 4 0.29964900635932423 1.0454883413695015 5 0.16122630259299814 0.7031569273792365 6 0.093511709410136 0.4893995970034745 7 0.0596872072736912 0.36443968281596384 8 0.04382156395777034 0.30579066338359784 9 0.03301298166786399 0.2591633715079612 >10 0.16539814285866614 2.5278731545269437 >50 0.009897485960234891 0.5692016970786166 >100 0.005623563124636062 0.9751564900292233 >500 4.4988504996803814E-4 0.29372419830361346 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 962 0.18806987910352055 No Hit GGTATCAACGCAGAGTACTTTTTTT 535 0.10459187663241526 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCACT 35 0.002112016 16.349052 4 TGGCGAG 170 0.0 14.490392 18 CCTAGCC 40 0.0051285196 14.309646 3 ATTATGC 40 0.0051285196 14.309646 3 CCTAACC 55 1.8879697E-4 13.8760195 3 TAGGACC 160 0.0 13.709362 4 CTCCTAG 85 2.5659938E-7 13.462603 1 AGTACCG 50 0.0014539646 13.3517275 5 CTAGACA 60 3.9520473E-4 12.715929 4 GGCGAGG 60 4.177381E-4 12.632649 19 GTAGGAC 245 0.0 12.460098 3 TGTAGGA 240 0.0 12.32462 2 TTAGGAC 125 1.3096724E-9 12.210897 3 ATGGCGA 195 0.0 12.146777 17 TAACATA 55 0.002975773 12.137933 4 GGCGAGA 145 1.0186341E-10 11.761432 19 CTATAAG 65 7.756962E-4 11.736628 1 ACTGTCC 100 1.919845E-6 11.400588 8 CTGTAGG 235 0.0 11.362055 1 TATGGCG 210 0.0 11.279151 16 >>END_MODULE