FastQCFastQC Report
Thu 26 May 2016
SRR1926604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874364
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC2950.014.8912911
GTCCTAA1353.7471182E-1014.7910121
GTTAACC450.004592883414.5500683
GACCTAA658.496418E-414.3359051
TCCTACA3850.013.4602172
TAGGACC1201.7334969E-913.3552954
GGGTAGG600.00827630613.3119121
ATATTAT600.00827630613.3119121
TAGATTG550.00154416613.2463595
GTGCGCC500.001908678412.87897211
CTTATAC851.28439315E-512.8382943
GTATAAA958.069164E-512.6112841
CTAGGAC704.6571792E-412.4714853
GTTTAAG750.002498142412.424451
CCTAGGA800.003380752911.932662
TTTAAGG1152.897727E-511.8585432
CTACTAA907.2429486E-411.8328091
GTCCTAG907.2429486E-411.8328091
CCTACAG4450.011.7708423
TTTGTAC650.00379124711.7519783
Produced by FastQC (version 0.11.5)