FastQCFastQC Report
Thu 26 May 2016
SRR1926604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874364
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15230.17418374955967994No Hit
GGTATCAACGCAGAGTACTTTTTTT8880.10155953355810624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAG400.00513634714.3079883
GTAGGAC5350.014.2634143
TGTAGGA6300.013.47682
GTCCTAC4050.013.1854611
TAGGACC2400.013.1141464
CTGTAGG6100.012.8187691
GCCGGTT604.1632217E-412.63962311
TCCAACG604.1813406E-412.63311818
GTATTAA1001.3705358E-712.3966881
TCCTACA4950.012.1415092
GGACGTG4200.012.03486
TAGGACG3850.011.8909854
GACGTGG2250.011.8233637
GTACTAA657.767355E-411.7365081
CCTACAG4900.011.6799913
AGGACGT4050.011.539275
TAGGACA2550.011.2206594
GACTAAT600.00570948311.1252331
AGGACCT3450.011.0580425
CGGTTTC700.001517076910.83396213
Produced by FastQC (version 0.11.5)