FastQCFastQC Report
Thu 26 May 2016
SRR1926603_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926603_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909606
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGA704.654932E-617.0593571
GCTTAAA552.4466185E-416.887041
GTCTTAA1053.3705874E-916.4275281
TAGGACC1700.015.7141784
TGTAGGA3250.015.4771192
GTCCTAC2650.014.5201231
GTGTAGC1201.967419E-814.3740871
CAACGCG450.006352920613.855825
CTGTAGG3100.013.6964011
GTAGGAC3350.013.6696423
GGACCTG2650.013.3289646
GTATTAG700.001524314313.2683891
TATTGGA956.6279215E-512.8792522
TATTAGC750.002133451612.688452
GTATATA1051.5106507E-512.636561
AGGACCT2650.012.5486675
GTATAGA1801.6916601E-1012.5312551
TGGACAG1750.012.4702385
GCATTAA750.002560560812.3838291
CCTACAG3450.012.3253193
Produced by FastQC (version 0.11.5)