FastQCFastQC Report
Thu 26 May 2016
SRR1926603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909606
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15930.17513077090520512No Hit
GGTATCAACGCAGAGTACTTTTTTT10160.1116967126426167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT1750.013.6261394
CTAGGAC905.132697E-712.7191383
GTCCTAA1750.012.5318871
TAGGACC2900.012.4984594
GTAGGAC4950.012.333713
CTACACT1700.011.7826034
TCTAGCC657.7444076E-411.7407433
ATAGGAC907.1156555E-611.659213
CTAAACT907.124434E-611.657924
GTCTTAT1406.384653E-1011.5783741
TGTAGGA5300.011.5198572
AATCCCG752.1258232E-411.36856819
GTCCTAC4200.011.3513471
TTATACT855.1034054E-511.2215274
TAAGACA855.1034054E-511.2215274
CCCTTAG855.120702E-511.2178021
ACTGATC855.31445E-511.176998
TCTATAC1201.2016972E-711.1292453
CAATACA1552.910383E-1011.0767324
GGACGTG3200.011.0280476
Produced by FastQC (version 0.11.5)