FastQCFastQC Report
Thu 26 May 2016
SRR1926602_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926602_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000877
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG500.00241504116.0245251
TATTAGC603.755696E-415.9641262
CTAGTCC601.2146509E-414.6166633
GTCCTAA1406.166374E-1014.307611
GTGTAGC1451.547778E-812.8932951
GTATAAT854.3626214E-412.56825351
TCTAGAC704.482697E-412.5285683
GTAGGAC3450.012.0746353
CTAGACT801.21743346E-412.0695354
AGAGCGC656.361388E-412.0186118
TGTTAGG800.003297212811.9730952
CTGTAGG3600.011.8700171
GTGTTAG800.00395725511.6845481
GTGCTAG800.00395725511.6845481
TGTAGGA3700.011.4645852
CCTCTAG1051.9932636E-411.4460881
TCTAAGA1102.4474968E-411.1956212
TAAAACT1351.2051714E-711.1258264
GTTTAGG850.006218480410.9972221
GGATAGA850.006218480410.9972221