FastQCFastQC Report
Thu 26 May 2016
SRR1926602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000877
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19150.1913322016591449No Hit
CCCATGTACTCTGCGTTGATACCAC13790.13777916766995343No Hit
GTACATGGGAAGCAGTGGTATCAAC13520.13508153349512478No Hit
GGTATCAACGCAGAGTACTTTTTTT10910.10900440313844759No Hit
TATCAACGCAGAGTACTTTTTTTTT10050.10041193872973402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC759.927517E-713.8999710
GCGTGCG551.9556281E-413.8210269
CCTATAC852.546476E-713.4746373
TAGGACC2600.013.2148444
AAGGCGT655.2270254E-513.2121836
GGTTAAT655.241862E-513.2081941
TCTAGGA1600.013.1263782
AAAGGCG603.9331615E-412.7254065
CCCTATA802.7244405E-511.9330712
GTAGGAC5000.011.6443323
TTAGGAC3200.011.3341343
AGGACCG600.00567429611.1347295
GGACCGG600.005682811611.1324886
TAGGACG4050.011.0758164
TGTAGGA6050.011.0454872
CTCCTAG1657.4578566E-1110.9845921
TATATTG1053.2748267E-610.907495
ACCGTGC700.001488034710.8593778
ACGGTTG700.001521337910.83060413
CTAGGAC1158.287898E-710.78947453
Produced by FastQC (version 0.11.5)