FastQCFastQC Report
Thu 26 May 2016
SRR1926601_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926601_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751937
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTAG352.2424373E-422.815381
CCTTAGG500.002103342416.3581562
GTCCTAA2100.015.2102521
GTGTAGG1055.76365E-815.2102521
CTAGGAC1154.1836756E-1115.1745953
TAGGACC2100.014.2400924
TGTAGGA3650.014.1920062
CTTAGGC550.001084203213.8813063
CATAGAA1006.90816E-613.6317962
ATCTTAT700.001488874713.3089721
GTCTTAG700.001488874713.3089721
GTCTAGG802.6871442E-413.3089711
GTAGGAC3800.012.9158763
TCTAGAC851.2906292E-512.8314593
ATAAGGG600.00208853712.7245293
AGTAGGA750.002089202212.7230092
CCTCTAG958.0807156E-512.6084991
TAGGACG3150.012.5448444
AGGACGT2750.012.47279555
GGACGTG2500.012.4671166
Produced by FastQC (version 0.11.5)