FastQCFastQC Report
Thu 26 May 2016
SRR1926601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751937
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13880.18458993240125168No Hit
CTGTAGGACGTGGAATATGGCAAGA9800.13033006754555237No Hit
GTCCTACAGTGGACATTTCTAAATT9580.12740429051901955No Hit
GGTATCAACGCAGAGTACTTTTTTT8050.1070568411981323No Hit
GTCCTACAGTGTGCATTTCTCATTT7920.10532797295518109No Hit
GTACATGGGAAGCAGTGGTATCAAC7580.10080631755053947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAA250.005888679519.0777382
TAGGACC2200.014.30542854
GGACCTG3950.013.2784136
CCTTAAG751.4222993E-512.7125321
GTCTTAC701.05329054E-412.2585131
ATAGGAC907.1203613E-611.6578373
CTAGCAC907.1203613E-611.6578373
AGGACCT4500.011.6562765
TCTTACA1156.6624125E-811.6125352
TCGAACT907.680956E-611.57876419
CCTAGGC855.1010255E-511.2214473
GTCCTAT855.1219482E-511.2169411
GACAGAT855.3029118E-511.1787857
GACCCAT600.00570642811.1256996
TGACGAA600.00599108311.0531918
GTCCTAC6150.011.0071941
CTGTAGG6500.011.001231
TGGCGAG6900.010.98453818
CTAGTAC700.001440738210.9008343
TAGGCTC700.001442349210.8993755
Produced by FastQC (version 0.11.5)