FastQCFastQC Report
Thu 26 May 2016
SRR1926600_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926600_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843892
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAGG300.00251762422.0903591
TTAGCCT400.002446585815.9936684
AAAGGCG400.002969001415.5363045
CTAATAC701.7933944E-615.5270023
TACTATA707.3213916E-515.5091282
GTGTAGC1151.1383236E-814.9830271
GTCCTAC4750.014.509881
ATTAGAC450.00471662414.4918693
GCTCTAA658.7799213E-414.2737711
GTCATAC852.9579687E-514.0338751
ATCTAGA852.9579687E-514.0338751
GTATAAG955.439131E-613.9518061
TGTAGGA5250.013.699732
CTAACCT550.001286847613.5703854
GTGTATA2450.013.5247121
TCCTACA5500.013.3237512
GTTATAA700.00153639113.2542171
CTGTAGG5200.013.2542161
GTCTAGA1101.6907743E-613.2542161
ATTGGAC600.002144402812.6803853
Produced by FastQC (version 0.11.5)