##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926600_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 843892 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.099235447189926 27.0 7.0 31.0 2.0 31.0 2 16.288361543894244 16.0 2.0 30.0 2.0 31.0 3 19.77617633536045 23.0 15.0 31.0 2.0 31.0 4 21.85241120901727 28.0 2.0 35.0 2.0 37.0 5 22.70692932270954 30.0 2.0 35.0 2.0 37.0 6 23.40779151834595 30.0 2.0 35.0 2.0 37.0 7 23.01477558739744 30.0 2.0 35.0 2.0 37.0 8 23.274133419916293 30.0 7.0 35.0 2.0 37.0 9 25.83121062884824 31.0 17.0 37.0 2.0 39.0 10 26.54624406914629 31.0 20.0 37.0 2.0 39.0 11 23.75109492683898 30.0 7.0 37.0 2.0 39.0 12 24.87528498907443 31.0 10.0 37.0 2.0 39.0 13 21.625389267821 27.0 2.0 35.0 2.0 39.0 14 24.824031985135537 30.0 12.0 38.0 2.0 40.0 15 25.906633787261878 31.0 16.0 38.0 2.0 40.0 16 26.248255700966475 31.0 12.0 38.0 2.0 40.0 17 25.548076057125794 31.0 10.0 38.0 2.0 40.0 18 25.573229749778406 31.0 10.0 38.0 2.0 40.0 19 25.9833912396373 31.0 10.0 38.0 2.0 40.0 20 26.244718518483406 31.0 10.0 39.0 2.0 40.0 21 24.107183146658578 30.0 2.0 37.0 2.0 40.0 22 25.010024979499747 31.0 2.0 38.0 2.0 40.0 23 24.984970825650677 31.0 2.0 38.0 2.0 40.0 24 24.10898550999417 31.0 2.0 38.0 2.0 40.0 25 23.89037578268309 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.463136904076286 1101 2 9.905290353368368 1101 3 6.517767789966051 1101 4 8.224035408973581 1101 5 8.01956085054827 1101 6 7.462184581584896 1101 7 7.1921338663928225 1101 8 5.480369618393812 1101 9 5.472101402419277 1101 10 5.346166328393494 1101 11 8.143746947705456 1101 12 5.496419784034554 1101 13 9.883037954873434 1101 14 7.49634308611191 1101 15 6.664915613475532 1101 16 5.957286375048589 1101 17 2.3311857384388652 1101 18 4.697156629393167 1101 19 5.343146976783846 1101 20 3.3353419453302884 1101 21 0.33119534951767804 1101 22 4.184569614694308 1101 23 5.354155954084515 1101 24 6.7905950719493475 1101 25 7.358162797825873 1102 1 5.567923315189802 1102 2 10.255365303868974 1102 3 6.592000849950502 1102 4 7.830577345181489 1102 5 7.668708338704491 1102 6 6.7479624234914475 1102 7 6.767429059139857 1102 8 6.9106416045420005 1102 9 5.860489285288079 1102 10 5.297620849071013 1102 11 7.8804622720767945 1102 12 5.348510782759995 1102 13 9.699882178073619 1102 14 6.951280600641084 1102 15 5.25790233080393 1102 16 5.592369622208885 1102 17 6.397290476753401 1102 18 6.2346719601773835 1102 19 5.07336926313484 1102 20 5.402023842579965 1102 21 4.330089128225325 1102 22 5.517778698534659 1102 23 5.833165805866894 1102 24 7.352210796323892 1102 25 7.159896679460196 1103 1 5.07454035186656 1103 2 9.146694496349525 1103 3 6.485261377275787 1103 4 8.134085730098295 1103 5 7.535861294222659 1103 6 7.043053997998889 1103 7 6.951813508173437 1103 8 6.517401010718551 1103 9 5.8458235435358965 1103 10 5.0826872010541315 1103 11 5.837730159736893 1103 12 4.991281610390132 1103 13 8.7693920708491 1103 14 7.204300526169522 1103 15 6.089096134939997 1103 16 5.64305653068222 1103 17 3.4054454938386236 1103 18 2.4245633713187047 1103 19 4.459154727270924 1103 20 4.901385970521154 1103 21 6.974463495336639 1103 22 2.8226452193766853 1103 23 4.862634609050584 1103 24 6.18960879216532 1103 25 2.5489490230641536 1104 1 5.700703497875427 1104 2 9.920152603858618 1104 3 6.818168781105044 1104 4 7.3129201666517005 1104 5 7.505691409114338 1104 6 7.094284880350546 1104 7 6.8101678319772 1104 8 6.873180960061646 1104 9 5.749891046440755 1104 10 4.576417163205711 1104 11 7.606445630376609 1104 12 4.660503125498462 1104 13 8.871203793979475 1104 14 6.570638077414394 1104 15 5.899358748238264 1104 16 5.515130797166915 1104 17 5.954758163398058 1104 18 3.559466450863333 1104 19 4.454199635657467 1104 20 3.493397234994088 1104 21 2.8842545989909496 1104 22 4.827568399063566 1104 23 5.106808059551689 1104 24 6.157571403627557 1104 25 5.232692213365027 1105 1 3.6008074809820627 1105 2 9.332014377263686 1105 3 6.36923426191445 1105 4 5.757425887885276 1105 5 2.583098267673954 1105 6 4.9914320060654305 1105 7 5.844384502666507 1105 8 3.0125589819084766 1105 9 3.9259828041791494 1105 10 3.6800023311661114 1105 11 6.973185915698643 1105 12 4.406068920765932 1105 13 5.072703071201726 1105 14 3.900200941776994 1105 15 4.482674131076902 1105 16 4.710303714498615 1105 17 5.467954155982557 1105 18 2.743727630128653 1105 19 2.7863162282296834 1105 20 2.034020718393556 1105 21 2.898762059043527 1105 22 4.376380335839759 1105 23 2.104620261908696 1105 24 4.857100884395443 1105 25 5.7525519486319965 1106 1 6.8153769262458646 1106 2 11.321228386270036 1106 3 7.888082707433888 1106 4 9.43794621117004 1106 5 9.04663040933718 1106 6 8.044103431546716 1106 7 7.515691996816312 1106 8 7.461323066623244 1106 9 6.379672851963939 1106 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2204 8 4.783523060564864 2204 9 3.2559547645858906 2204 10 3.1253303216446042 2204 11 4.302096321532126 2204 12 4.485294414707148 2204 13 2.2657799682920015 2204 14 3.7029921676451423 2204 15 3.947477814944129 2204 16 3.9464976580504185 2204 17 -1.6358975849929287 2204 18 2.1979170675956823 2204 19 3.1173562398717003 2204 20 3.444262835993534 2204 21 4.742774605397212 2204 22 4.211814689668763 2204 23 3.0766118526293837 2204 24 4.623813547429176 2204 25 4.602093154360492 2205 1 5.741040537695557 2205 2 10.076836220201564 2205 3 6.960882691558975 2205 4 7.519864137028286 2205 5 7.6675586104013895 2205 6 7.11833553543282 2205 7 7.017172036858245 2205 8 6.836478068677273 2205 9 5.524737886329326 2205 10 5.025813130740595 2205 11 7.306124935039744 2205 12 6.466909855358672 2205 13 9.43395426378725 2205 14 7.053934109498449 2205 15 5.901750613418919 2205 16 5.542610974247815 2205 17 6.131538744477332 2205 18 6.1953911637134915 2205 19 4.676952813881968 2205 20 5.203959333991417 2205 21 -0.8416156080405983 2205 22 3.537347022307845 2205 23 4.930815226176133 2205 24 6.642229760780577 2205 25 7.067427529709384 2206 1 4.369681814705327 2206 2 8.815293686998412 2206 3 6.0665792897679545 2206 4 2.416654647335111 2206 5 6.010705786659514 2206 6 6.174915557640357 2206 7 6.384568097013698 2206 8 6.331807529970586 2206 9 5.2424099290071595 2206 10 4.180111409020217 2206 11 4.047145854065359 2206 12 5.05079547890443 2206 13 8.229530400099797 2206 14 0.2856262509311165 2206 15 2.6972465888328507 2206 16 3.913696980530055 2206 17 4.600866809528959 2206 18 -0.70061088569107 2206 19 1.700525207656053 2206 20 3.1651924206780073 2206 21 3.974574603168527 2206 22 -1.1322831941944393 2206 23 -2.0782594965053285 2206 24 1.742741772615549 2206 25 3.3019728053483597 2207 1 1.3552698422366518 2207 2 -13.52108180191945 2207 3 -6.078727914158325 2207 4 -4.351064199466762 2207 5 1.0305122708883978 2207 6 1.7014576713104894 2207 7 2.929294049808071 2207 8 3.003701479963418 2207 9 0.6185189796846693 2207 10 0.2556757727982806 2207 11 -1.572508021700365 2207 12 -2.2558792959809217 2207 13 0.3822641772965909 2207 14 -5.113613968351281 2207 15 -1.5651717317359015 2207 16 -0.4577342516759977 2207 17 0.6099426356789088 2207 18 0.4259356912187009 2207 19 -0.027484110215016955 2207 20 -0.3390364988832566 2207 21 -3.8757073104969706 2207 22 -4.689958121001602 2207 23 -1.4836177873592078 2207 24 0.1696244658030146 2207 25 0.7822100952005826 2208 1 4.57516597273186 2208 2 6.155108674271027 2208 3 6.064530077210268 2208 4 8.07907843823348 2208 5 3.500207245652497 2208 6 6.114068306962601 2208 7 6.441669150385938 2208 8 6.684362733712231 2208 9 5.117004825635952 2208 10 4.551452233920362 2208 11 6.64060294311567 2208 12 6.17847371404029 2208 13 8.676340689621952 2208 14 3.4455111870872415 2208 15 4.742586296206458 2208 16 5.354601349231032 2208 17 6.272899259188531 2208 18 1.2545978227379742 2208 19 3.5505862577171037 2208 20 4.781060055867677 2208 21 7.218202425440417 2208 22 6.251438996985378 2208 23 2.4745154874560953 2208 24 4.050262312339477 2208 25 5.2928003958369665 2209 1 3.2310364006131564 2209 2 4.989108415471854 2209 3 5.299199676934215 2209 4 0.7157386349208501 2209 5 5.443420237370926 2209 6 5.604656643746587 2209 7 6.254051828094703 2209 8 4.861682005623894 2209 9 4.618732310108001 2209 10 3.7950797356456896 2209 11 -0.08264412382704833 2209 12 3.3598526822942603 2209 13 2.2586590988697104 2209 14 3.928939413450248 2209 15 4.445433518428679 2209 16 3.917834182219302 2209 17 1.685818945178866 2209 18 3.580272425912579 2209 19 4.0964282176895 2209 20 4.294890283610947 2209 21 6.52870277050598 2209 22 5.138835045984688 2209 23 0.9412679891814228 2209 24 -2.4025952618496156 2209 25 0.3827602819653322 2210 1 -2.3855608504490426 2210 2 -13.52108180191945 2210 3 -7.52395924553328 2210 4 -2.4916251760557344 2210 5 -0.8258905115988213 2210 6 -1.430353978238525 2210 7 -1.2742661446235672 2210 8 -20.441986472636977 2210 9 -12.888936184125777 2210 10 -7.173288911129379 2210 11 -10.037301025721437 2210 12 -4.78908422071823 2210 13 -5.440934889725634 2210 14 -4.327851069890006 2210 15 -5.841040122276684 2210 16 -5.354208932657869 2210 17 -4.681817044183173 2210 18 -4.8000086174379 2210 19 -9.83194526089083 2210 20 -7.058702023835625 2210 21 -5.426896533075148 2210 22 -9.706210257181143 2210 23 -7.941834768541479 2210 24 -10.634127083879454 2210 25 -8.279015845722112 2211 1 -0.1898863552729182 2211 2 4.463304287505675 2211 3 0.3973005887742467 2211 4 1.4031407586904052 2211 5 0.6591920035016976 2211 6 -0.5250047719969224 2211 7 -20.178013389296602 2211 8 -13.38165999996132 2211 9 -9.3528724936381 2211 10 -7.89191207939427 2211 11 -9.980617555493048 2211 12 -5.560270188536116 2211 13 -7.1227134633853595 2211 14 -5.452993887996968 2211 15 -5.931884171422279 2211 16 -12.277479041004078 2211 17 -8.561268393204816 2211 18 -6.598977041795017 2211 19 -9.444531297230117 2211 20 -7.381553077391306 2211 21 -5.219997356009898 2211 22 -6.5521330511856775 2211 23 -8.492851664751369 2211 24 -21.41957895750179 2211 25 -21.196723413686843 2212 1 -1.7135334225261225 2212 2 -13.52108180191945 2212 3 -7.838443101252963 2212 4 -19.03901679986176 2212 5 -19.795030849585597 2212 6 -20.479582486688194 2212 7 -9.984940434248703 2212 8 -9.411035846255253 2212 9 -5.486658717894944 2212 10 -5.886778223086807 2212 11 -5.983804772316365 2212 12 -3.2988729072414813 2212 13 -6.035590624785412 2212 14 -4.219503843734717 2212 15 -4.335206219964835 2212 16 -4.336713993904503 2212 17 -4.682790579507941 2212 18 -3.5828171098280244 2212 19 -4.27912447206732 2212 20 -4.358389008946165 2212 21 -2.3359803540334454 2212 22 -3.6658697255702144 2212 23 -3.6176280130293357 2212 24 -2.6097042338466707 2212 25 -3.1959864940110876 2213 1 -18.370865773299887 2213 2 -13.52108180191945 2213 3 -8.543904942631004 2213 4 -5.90776679986176 2213 5 -19.795030849585597 2213 6 -10.776457486688194 2213 7 -20.178013389296602 2213 8 -10.633392722636977 2213 9 -8.667035352935475 2213 10 -7.488446079571702 2213 11 -6.5937174933922655 2213 12 -5.35889731770574 2213 13 -18.70397677069551 2213 14 -12.26277254307149 2213 15 -7.479566481571322 2213 16 -6.671398154737222 2213 17 -5.125763935255915 2213 18 -5.168946324087422 2213 19 -6.022491619267022 2213 20 -6.020361075084704 2213 21 -4.130366522972281 2213 22 -4.790173106189226 2213 23 -4.696164076035966 2213 24 -3.8969227075017905 2213 25 -4.767035913686843 2214 1 -18.370865773299887 2214 2 -13.52108180191945 2214 3 -11.93803164291243 2214 4 -19.03901679986176 2214 5 -19.795030849585597 2214 6 -20.479582486688194 2214 7 -15.959439280478591 2214 8 -14.58645176344373 2214 9 -13.431454032579003 2214 10 -13.488362531260256 2214 11 -13.85522957125343 2214 12 -11.919726351476847 2214 13 -18.70397677069551 2214 14 -16.271326717555542 2214 15 -23.19519148157132 2214 16 -17.694151144887314 2214 17 -22.851545185255915 2214 18 -17.25521068853395 2214 19 -14.524615505289537 2214 20 -13.241205645910222 2214 21 -10.735032036574534 2214 22 -11.764155868853392 2214 23 -11.679445619187938 2214 24 -10.592186837426745 2214 25 -10.387154933386656 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 159267.0 3 2.0 4 668.0 5 2931.0 6 4212.0 7 3962.0 8 3473.0 9 3270.0 10 3022.0 11 2731.0 12 2741.0 13 2715.0 14 4220.0 15 8332.0 16 11852.0 17 12592.0 18 13017.0 19 15506.0 20 18397.0 21 21951.0 22 22890.0 23 24524.0 24 26291.0 25 26653.0 26 22786.0 27 18258.0 28 19712.0 29 22440.0 30 21856.0 31 25373.0 32 31882.0 33 39909.0 34 47661.0 35 53985.0 36 53003.0 37 53740.0 38 38068.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.173793246966646 18.707796919825466 12.313536200901865 25.804873632306023 2 14.433572834195232 20.23614479886504 41.16754381950483 24.162738547434902 3 19.02503987758393 24.127373621134247 28.67054750237768 28.17703899890415 4 11.32582046843537 17.518906712392422 38.12321496993133 33.03205784924088 5 12.409142478707178 37.41986353051021 37.13229506505914 13.03869892572347 6 32.21586139928352 37.40104455258148 17.893860510536047 12.489233537598956 7 28.619790886485514 31.12527142163063 21.96499491349026 18.289942778393602 8 24.312478196476423 38.781456175077345 19.99956552685619 16.906500101590044 9 25.971037323135032 15.950468318513527 19.975658824980783 38.10283553337066 10 13.848147126216153 30.072819319313314 34.932999028275965 21.14603452619456 11 33.453978868096016 22.68290033696685 24.395950405071858 19.467170389865277 12 23.037408208853655 25.063961931257 32.069244955779176 19.82938490411017 13 28.020236829971907 21.50525352452086 25.32027485760532 25.15423478790192 14 20.11364045888642 22.50105111418103 28.124211664364225 29.26109676256832 15 22.309436404196987 31.528230877956098 23.63321875068421 22.529113967162708 16 21.528334308232814 28.94040790118532 27.83437329367256 21.696884496909306 17 21.10862663704752 28.694459541778684 28.824195291820327 21.372718529353474 18 21.016954442438443 28.13436311604502 30.459056774094417 20.389625667422116 19 22.402648040483633 27.382840524905163 27.611748700094296 22.602762734516904 20 22.55093745840385 28.22108710947156 28.47434824773242 20.753627184392172 21 21.686338018085628 26.658034477736116 27.074872924139598 24.58075458003866 22 21.903490601856078 28.479788343514056 28.2608747891365 21.355846265493362 23 21.79479119093233 28.009300173785135 28.3251976583491 21.870710976933434 24 22.011529873679677 27.80123286143664 28.648987181249304 21.538250083634374 25 22.21209942798304 27.722418746782257 28.368340012005 21.6971418132297 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 748.0 1 748.0 2 1653.5 3 2559.0 4 2559.0 5 2559.0 6 3570.5 7 4582.0 8 4582.0 9 4582.0 10 5591.5 11 6601.0 12 6601.0 13 6601.0 14 8373.0 15 10145.0 16 10145.0 17 10145.0 18 14430.5 19 18716.0 20 18716.0 21 18716.0 22 26158.5 23 33601.0 24 33601.0 25 33601.0 26 43590.5 27 53580.0 28 53580.0 29 53580.0 30 64639.0 31 75698.0 32 75698.0 33 75698.0 34 83662.5 35 91627.0 36 91627.0 37 91627.0 38 96756.5 39 101886.0 40 101886.0 41 101886.0 42 103161.0 43 104436.0 44 104436.0 45 104436.0 46 102379.0 47 100322.0 48 100322.0 49 100322.0 50 93356.0 51 86390.0 52 86390.0 53 86390.0 54 75105.0 55 63820.0 56 63820.0 57 63820.0 58 53165.5 59 42511.0 60 42511.0 61 42511.0 62 33640.5 63 24770.0 64 24770.0 65 24770.0 66 18838.5 67 12907.0 68 12907.0 69 12907.0 70 9315.0 71 5723.0 72 5723.0 73 5723.0 74 3932.5 75 2142.0 76 2142.0 77 2142.0 78 1465.5 79 789.0 80 789.0 81 789.0 82 517.0 83 245.0 84 245.0 85 245.0 86 153.5 87 62.0 88 62.0 89 62.0 90 44.5 91 27.0 92 27.0 93 27.0 94 16.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.308328553890782 2 35.26719058836913 3 5.058704194375584 4 12.787181298080796 5 12.682191560057449 6 13.600792518473929 7 9.02662899991942 8 7.26811013731615 9 0.10380475226687774 10 1.22314229782958 11 5.086314362501363 12 5.0984012172173685 13 14.929991041507682 14 1.3558607025543554 15 2.580661980442995 16 2.557791755343101 17 2.5419129462063865 18 1.2840505657122 19 1.228830229460642 20 0.29245448469709395 21 3.998852933787736 22 1.3745834775066004 23 0.1062932223554673 24 8.61223948088144 25 0.10783370383887986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 843892.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.16877297040453 #Duplication Level Percentage of deduplicated Percentage of total 1 97.23593814689335 91.56588983919096 2 2.105306214205778 3.965082058374515 3 0.31592406757008346 0.892505453846818 4 0.11375148419077155 0.4284735075922931 5 0.06846776743014642 0.32237628234599586 6 0.03727194204344241 0.21059118290730022 7 0.02275654536143212 0.15000691676119896 8 0.014359175416474744 0.10817487438689792 9 0.013657184551792345 0.11574722803253845 >10 0.06382635138938297 1.0845084812597956 >50 0.004885356870469851 0.3324240725848521 >100 0.0037272386185188476 0.7496651009044154 >500 1.2852545836627492E-4 0.07455500181243528 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.1849857564712073E-4 0.0 5 0.0 0.0 0.0 1.1849857564712073E-4 0.0 6 0.0 0.0 0.0 1.1849857564712073E-4 0.0 7 0.0 0.0 0.0 1.1849857564712073E-4 0.0 8 0.0 0.0 0.0 1.1849857564712073E-4 0.0 9 0.0 0.0 0.0 2.3699715129424145E-4 0.0 10 0.0 0.0 0.0 2.3699715129424145E-4 0.0 11 0.0 0.0 0.0 2.3699715129424145E-4 0.0 12 0.0 0.0 0.0 2.3699715129424145E-4 0.0 13 0.0 0.0 0.0 2.3699715129424145E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTAGG 30 0.002517624 22.090359 1 TTAGCCT 40 0.0024465858 15.993668 4 AAAGGCG 40 0.0029690014 15.536304 5 CTAATAC 70 1.7933944E-6 15.527002 3 TACTATA 70 7.3213916E-5 15.509128 2 GTGTAGC 115 1.1383236E-8 14.983027 1 GTCCTAC 475 0.0 14.50988 1 ATTAGAC 45 0.004716624 14.491869 3 GCTCTAA 65 8.7799213E-4 14.273771 1 GTCATAC 85 2.9579687E-5 14.033875 1 ATCTAGA 85 2.9579687E-5 14.033875 1 GTATAAG 95 5.439131E-6 13.951806 1 TGTAGGA 525 0.0 13.69973 2 CTAACCT 55 0.0012868476 13.570385 4 GTGTATA 245 0.0 13.524712 1 TCCTACA 550 0.0 13.323751 2 GTTATAA 70 0.001536391 13.254217 1 CTGTAGG 520 0.0 13.254216 1 GTCTAGA 110 1.6907743E-6 13.254216 1 ATTGGAC 60 0.0021444028 12.680385 3 >>END_MODULE