FastQCFastQC Report
Thu 26 May 2016
SRR1926600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843892
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14390.17051945035620672No Hit
GTCCTACAGTGGACATTTCTAAATT12750.15108568395007893No Hit
CTGTAGGACGTGGAATATGGCAAGA12390.14681973522678257No Hit
GTCCTACAGTGTGCATTTCTCATTT9760.11565460983158982No Hit
GTCCTAAAGTGTGTATTTCTCATTT8630.10226427078346519No Hit
GGTATCAACGCAGAGTACTTTTTTT8610.10202727363217093No Hit
CTTTAGGACGTGAAATATGGCGAGG8590.10179027648087671No Hit
CTGTAGGACCTGGAATATGGCGAGA8540.10119778360264109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG2500.013.265408519
GATATAC2300.013.2639651
TAGGACC3600.013.2393594
TCCAACG802.0555399E-613.02852618
GCCTATA751.4242123E-512.71131
GTCTTAT701.05454295E-412.2573251
TGTAGGA8550.012.1595852
CTACACT2000.011.9154224
GGACCTG4650.011.6841046
GCCATAT2450.011.6736411
TTAATAC907.153447E-611.6534143
GTAGCCC1501.6552804E-1011.4415333
TTAGGAC3950.011.344983
ACTGTTC3100.011.3408028
CTGTAGG8500.011.32801151
TGGCGAG6450.011.31158918
GTAGGAC8250.011.0948213
CGTGGAA2750.011.0565849
GACGTGG2750.011.0546157
TAGGACG6300.011.0454084
Produced by FastQC (version 0.11.5)