##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926600_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 843892 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.28453166992933 31.0 28.0 31.0 22.0 34.0 2 28.763646295971522 31.0 28.0 33.0 23.0 34.0 3 29.130082996402383 31.0 28.0 33.0 25.0 34.0 4 32.91602124442464 35.0 33.0 37.0 27.0 37.0 5 32.62789432771018 35.0 33.0 37.0 26.0 37.0 6 32.73038137581586 35.0 33.0 37.0 26.0 37.0 7 32.563036502301244 35.0 33.0 37.0 25.0 37.0 8 32.523273120257095 35.0 33.0 37.0 25.0 37.0 9 33.758612476478035 37.0 34.0 39.0 23.0 39.0 10 33.65615268304475 37.0 33.0 39.0 23.0 39.0 11 33.902634460333786 37.0 34.0 39.0 25.0 39.0 12 33.71718063448877 37.0 34.0 39.0 23.0 39.0 13 33.84310196091443 37.0 34.0 39.0 25.0 39.0 14 34.47945590194006 38.0 34.0 40.0 22.0 41.0 15 34.599316026221366 38.0 34.0 40.0 24.0 41.0 16 34.57283040957848 38.0 34.0 40.0 23.0 41.0 17 34.496908372161364 38.0 34.0 40.0 22.0 41.0 18 34.46037170633209 38.0 34.0 40.0 22.0 41.0 19 34.50997284012646 39.0 34.0 40.0 21.0 41.0 20 34.48311276798453 39.0 34.0 40.0 19.0 41.0 21 34.40488356329957 39.0 34.0 40.0 19.0 41.0 22 34.33352609101639 39.0 34.0 40.0 18.0 41.0 23 33.988543557706436 39.0 34.0 40.0 16.0 41.0 24 34.067532338261294 39.0 34.0 40.0 17.0 41.0 25 33.85501580770999 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8436376455290002 1101 2 0.8749056347785391 1101 3 0.9387169766984975 1101 4 0.5816728722530442 1101 5 0.7528061872944107 1101 6 0.1717054201135042 1101 7 0.6347638351483909 1101 8 0.5103403558294062 1101 9 1.054541494235707 1101 10 0.9714419499889857 1101 11 0.717102444592939 1101 12 0.7543579817623325 1101 13 0.6842687713238647 1101 14 1.0761913475495177 1101 15 0.8844544365437699 1101 16 0.8792733448504819 1101 17 0.8683602252911413 1101 18 0.9384047094549786 1101 19 0.9449846358345297 1101 20 0.9102404350823932 1101 21 1.1321896704324104 1101 22 0.9380962120093699 1101 23 1.2238372386950545 1101 24 1.123920041187212 1101 25 1.1983113966127945 1102 1 0.9398989382046921 1102 2 0.8944128051766995 1102 3 0.7841666396216453 1102 4 0.8069211708366026 1102 5 0.9379860284153807 1102 6 0.3555385570524763 1102 7 0.6123468217855574 1102 8 0.9938191813109682 1102 9 1.2842868228555702 1102 10 1.1563011472571034 1102 11 1.030848446564896 1102 12 1.0392059564955929 1102 13 0.8335204687322602 1102 14 1.233407036653233 1102 15 1.2096794884479962 1102 16 1.2020494793285152 1102 17 1.0939251596793937 1102 18 1.1026465069844207 1102 19 1.0815588769106057 1102 20 0.9751507750450372 1102 21 1.0108500204151767 1102 22 1.0399086513876412 1102 23 1.2217610697478136 1102 24 1.0790379178836247 1102 25 1.3685051456608832 1103 1 0.22161114627855838 1103 2 0.10182930816716862 1103 3 -0.09947752345034644 1103 4 -0.09280667041830526 1103 5 0.09543101416514332 1103 6 -0.18704516144040895 1103 7 -0.062050942827248434 1103 8 0.046022364824104045 1103 9 0.3987091101053082 1103 10 0.46365581209520457 1103 11 -0.0065636356178302435 1103 12 0.10418872952033809 1103 13 -0.007652932357260056 1103 14 0.3389788490869563 1103 15 0.1342329558584865 1103 16 0.056670906691273615 1103 17 -0.0016506660327024747 1103 18 0.11889319691569966 1103 19 0.20302344887630852 1103 20 0.08456222289943582 1103 21 -0.03491995760481359 1103 22 -0.006555898429724039 1103 23 0.49740109019515444 1103 24 0.12830954772646663 1103 25 0.0836057921264981 1104 1 0.33541126221065554 1104 2 0.485488478299704 1104 3 0.19760764991884727 1104 4 0.006411880815200277 1104 5 0.07807311185410981 1104 6 -0.1965199435868641 1104 7 0.039076203905537454 1104 8 0.268156914249559 1104 9 0.5520179397419582 1104 10 0.6849064448693625 1104 11 0.3623382356450833 1104 12 0.43245447805610837 1104 13 0.19126765765491882 1104 14 0.49973845785547866 1104 15 0.4154666447110884 1104 16 0.5462204064608969 1104 17 0.3881456484457857 1104 18 0.35409065305428555 1104 19 0.26357086519055883 1104 20 0.46056121920377535 1104 21 0.47239311937811834 1104 22 0.4494917613768976 1104 23 0.6989598785211584 1104 24 0.4446848249886486 1104 25 0.5965921976457764 1105 1 0.8878725561861209 1105 2 0.9404643121758767 1105 3 0.7956969387072697 1105 4 0.6540017133089719 1105 5 0.8811773795734084 1105 6 0.4565999389001121 1105 7 0.47412655612451715 1105 8 0.637997228378893 1105 9 0.9681246807366506 1105 10 0.9990543759775292 1105 11 0.8291977295535986 1105 12 0.7565564991520475 1105 13 0.8616079038751465 1105 14 1.0933985161448447 1105 15 1.1950618199641454 1105 16 1.0343151193265143 1105 17 1.0361671578963936 1105 18 0.9965609179516832 1105 19 1.118715130937467 1105 20 1.140473620789649 1105 21 1.1058418549279736 1105 22 1.2137204480841035 1105 23 1.4897652458488366 1105 24 1.1152106317444392 1105 25 1.3150021384999775 1106 1 0.7452162723877223 1106 2 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1107 14 1.2082659972330632 1107 15 1.1095956324912706 1107 16 0.8847944430499837 1107 17 0.9657121576755117 1107 18 1.0775015306648683 1107 19 1.1815482472822936 1107 20 0.9626274981274676 1107 21 1.1314529959237305 1107 22 1.0933048176350297 1107 23 1.3800756043866187 1107 24 1.2840952588230437 1107 25 1.3852960215028887 1108 1 1.2888434852598962 1108 2 1.1112103822556776 1108 3 0.9810263582982408 1108 4 0.6742356577762791 1108 5 -0.5751618735540909 1108 6 0.2907434583174009 1108 7 0.744429741734784 1108 8 0.8020573219256164 1108 9 1.182325715600534 1108 10 1.248952779160291 1108 11 0.975596801851701 1108 12 0.8483094260463417 1108 13 0.9354319045377935 1108 14 1.2676881796717154 1108 15 1.1256563101810855 1108 16 1.150056543131491 1108 17 1.2342770688276943 1108 18 1.1919322446119764 1108 19 1.1820468335448169 1108 20 1.0078528888266405 1108 21 1.2878088728478403 1108 22 1.4367351686216736 1108 23 1.5293763565285374 1108 24 1.4378174119406921 1108 25 1.3934245275911934 1109 1 0.7588225919396159 1109 2 -0.3074401486330771 1109 3 0.6056078433850729 1109 4 0.7485209455748247 1109 5 -0.6430622063636129 1109 6 0.2829272357987591 1109 7 0.803793439784215 1109 8 1.1134770164398802 1109 9 1.4456388928642667 1109 10 -0.8892935563169857 1109 11 0.4142058919705889 1109 12 0.8680204146767494 1109 13 1.0396937140877327 1109 14 1.2913689446648675 1109 15 1.0467022231500067 1109 16 1.2687962267417277 1109 17 1.1192809803521513 1109 18 1.2908062079669804 1109 19 1.3082068087513505 1109 20 1.4292881338691998 1109 21 1.3807065547405486 1109 22 1.1604477446165191 1109 23 1.2625698852879168 1109 24 1.0549817910823265 1109 25 1.2289602835666784 1110 1 0.767687882298361 1110 2 0.05335985641035634 1110 3 0.6241196714505222 1110 4 0.8436376889518016 1110 5 1.1695043827279221 1110 6 0.9129341171767038 1110 7 1.1523461439135758 1110 8 1.2426975585411597 1110 9 1.4980755747307484 1110 10 1.1781221249806535 1110 11 0.9558265898363913 1110 12 1.1517591706070291 1110 13 1.1336102171256783 1110 14 1.49217909477008 1110 15 1.2540281022230033 1110 16 0.740009613955749 1110 17 1.1694015620734888 1110 18 1.249315717992502 1110 19 1.192364945629187 1110 20 1.0969369770072461 1110 21 1.1216277670851298 1110 22 1.1318257708588675 1110 23 1.5140093250399715 1110 24 1.3040338436872858 1110 25 1.4066642310722912 1111 1 0.17806207681042352 1111 2 0.1407784907509253 1111 3 0.4631845235861576 1111 4 0.32790462199448456 1111 5 0.6348816463211548 1111 6 0.4139425962427694 1111 7 0.5865518120280342 1111 8 0.6414937318753928 1111 9 0.779202009205278 1111 10 0.8400293430394541 1111 11 0.3288126074293416 1111 12 0.38090027132190585 1111 13 0.26325987509233784 1111 14 0.6906317177258714 1111 15 0.6494349830329611 1111 16 0.5151806306268085 1111 17 0.47757792104629004 1111 18 0.5536299358902639 1111 19 0.2337450285760596 1111 20 0.28999221813432996 1111 21 0.4456513208243962 1111 22 0.46511398208633636 1111 23 0.7341961772362922 1111 24 0.7004746430954043 1111 25 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0.5979097901870887 2203 1 0.16175357147957925 2203 2 0.2857645400127353 2203 3 0.3194951538638797 2203 4 0.007531000900229401 2203 5 0.23393234940139962 2203 6 0.11462627480297982 2203 7 0.3084798779568203 2203 8 0.2683392020429878 2203 9 0.7093041914333327 2203 10 0.7188487040771676 2203 11 0.610536216513033 2203 12 0.7562134700136767 2203 13 0.4285932145849358 2203 14 0.8717270816461209 2203 15 0.8294045870537303 2203 16 0.8424335362606925 2203 17 0.7945336112844643 2203 18 0.7256707163924929 2203 19 0.748125375589126 2203 20 0.7938522303296764 2203 21 0.851279658376825 2203 22 0.8690271179535927 2203 23 1.0253674849101202 2203 24 0.9756742033237913 2203 25 0.570483325580966 2204 1 0.5510946926524056 2204 2 0.5224781734539512 2204 3 0.3050105533720533 2204 4 0.3033326870546631 2204 5 0.48849054207051523 2204 6 0.33929491022384894 2204 7 0.6669279947453575 2204 8 0.5090293920531934 2204 9 1.120236225824783 2204 10 0.9145912731197754 2204 11 0.6754724136209234 2204 12 0.7052978358532513 2204 13 0.44427922734914915 2204 14 0.9281871017695522 2204 15 0.621758683115857 2204 16 0.4850574279283535 2204 17 0.6514452280239666 2204 18 0.592294430073153 2204 19 0.6121859856123351 2204 20 0.645073257496307 2204 21 0.6252071050624508 2204 22 0.5725947584634312 2204 23 0.7732379975490176 2204 24 0.6174285921563794 2204 25 0.563468276598222 2205 1 0.4952151113236489 2205 2 0.47714151388377246 2205 3 0.6000027617820116 2205 4 0.4622896791030868 2205 5 0.3040234179607353 2205 6 0.22714905542247266 2205 7 0.12642947841980146 2205 8 0.3250409277984758 2205 9 0.7674895780478579 2205 10 0.591572439415792 2205 11 0.5334900945203387 2205 12 0.47867228516984284 2205 13 0.5993260945225316 2205 14 0.8174400116920992 2205 15 0.8290051398993725 2205 16 0.8170091068096141 2205 17 0.7099734794354546 2205 18 0.710528119599573 2205 19 0.6450248748648164 2205 20 0.8427995590212163 2205 21 0.7788274033201574 2205 22 0.6715622818841851 2205 23 1.0094093688988082 2205 24 0.8550928347252764 2205 25 0.9802424171599569 2206 1 0.6165066094332907 2206 2 0.6129739445233859 2206 3 0.6382993129971908 2206 4 0.601658944311815 2206 5 0.7678729733434295 2206 6 0.7152407085946777 2206 7 0.8302794918647791 2206 8 0.7339204650537852 2206 9 1.0491300178215752 2206 10 1.124979175815369 2206 11 0.9630396920617343 2206 12 1.0028774834980894 2206 13 0.7890067148406459 2206 14 1.068716860291488 2206 15 0.9886504470089221 2206 16 1.041108865987617 2206 17 1.0151778303484846 2206 18 0.9061156226427158 2206 19 0.9292554871668415 2206 20 0.8738366713966172 2206 21 1.1087259764561637 2206 22 1.2872895957136805 2206 23 1.5546485325449453 2206 24 1.303167234218357 2206 25 1.3264475832541862 2207 1 -0.8916400903872344 2207 2 0.0855692239608139 2207 3 0.38833815176523245 2207 4 0.4667364973921835 2207 5 0.36279320580955243 2207 6 0.32747530677007575 2207 7 0.4386319298665171 2207 8 0.42137223846197713 2207 9 0.9908066337932766 2207 10 0.6596933096078459 2207 11 0.5371599403208549 2207 12 0.5970295237565111 2207 13 0.2825521836653522 2207 14 -5.10701547579389 2207 15 -2.0812151322294596 2207 16 -0.8929223063080229 2207 17 -0.681006872240971 2207 18 0.04771533356211677 2207 19 0.1743590128686705 2207 20 0.4001830891747318 2207 21 0.38935173982034144 2207 22 0.4353240965561156 2207 23 0.6851047357184825 2207 24 0.3192645088097379 2207 25 0.3959582491094622 2208 1 0.6360989191950921 2208 2 0.8564126150807034 2208 3 0.9319852880390975 2208 4 0.810202227770354 2208 5 0.7299031465600478 2208 6 0.6807281778109626 2208 7 0.8403289527617019 2208 8 0.8640026239495029 2208 9 1.0639161356585376 2208 10 1.0073723382085333 2208 11 0.7844308056562355 2208 12 0.8331761114238319 2208 13 0.7578736670974351 2208 14 0.9908939675533404 2208 15 0.9633873108983337 2208 16 0.958760818554822 2208 17 0.6081804586053465 2208 18 0.9121817222681372 2208 19 0.6512955939527174 2208 20 0.7515370501139458 2208 21 1.0538649429510585 2208 22 1.0423608897680268 2208 23 1.4980694054573434 2208 24 1.5067006875823168 2208 25 1.3978103447864427 2209 1 -0.2686038930892387 2209 2 -0.05346272998486157 2209 3 0.4307430520142539 2209 4 0.49841047483729994 2209 5 0.5724652583612837 2209 6 0.5757946029317367 2209 7 0.8995012267437588 2209 8 0.9086864576279012 2209 9 1.1835602867972526 2209 10 0.5591869517351071 2209 11 0.6225730589343641 2209 12 0.7176895689807665 2209 13 0.7793656443231072 2209 14 1.1631860655519617 2209 15 1.1458605683172394 2209 16 0.8928666674161079 2209 17 1.1393045096750498 2209 18 1.067645404117691 2209 19 1.184570697472381 2209 20 0.8076343586025558 2209 21 0.9829004847039897 2209 22 0.8785755205478694 2209 23 1.251211227403516 2209 24 1.0621803287141347 2209 25 1.2275515456146024 2210 1 -0.11468843370835202 2210 2 -0.45781245285487415 2210 3 0.3865460101971756 2210 4 0.45971110896181955 2210 5 0.7671530678364178 2210 6 0.6946819699572586 2210 7 0.8142183664747691 2210 8 0.8711915489929467 2210 9 1.3228196029969794 2210 10 1.1679340167128984 2210 11 0.8916871414504115 2210 12 0.6290558916974405 2210 13 0.7822143129810613 2210 14 1.1311134606898037 2210 15 0.7948828265672461 2210 16 1.0206214952281343 2210 17 0.9552077378001513 2210 18 1.0461872970672488 2210 19 1.0664595140394866 2210 20 1.3099863695919183 2210 21 1.154124089470308 2210 22 1.0750702654605035 2210 23 1.316989667169672 2210 24 1.3529911136229273 2210 25 1.2961512005997449 2211 1 -1.462951854177895 2211 2 -0.5621370711765792 2211 3 0.33068750845884765 2211 4 0.5494517236415675 2211 5 0.8480943662700753 2211 6 0.5497826687817096 2211 7 0.58121013608433 2211 8 0.5590875090982621 2211 9 1.0032746810639281 2211 10 0.9758827080531844 2211 11 0.8235119957381372 2211 12 0.7352937902909815 2211 13 0.7507737313104812 2211 14 1.016567472346786 2211 15 0.604892374717096 2211 16 0.868685452709407 2211 17 0.8737976361600701 2211 18 0.8967115588064871 2211 19 1.0207639774736137 2211 20 0.9109800863774211 2211 21 0.9496919957972736 2211 22 0.9893501988581122 2211 23 1.1941267240554723 2211 24 0.7941286732076094 2211 25 0.9509253537424698 2212 1 0.7543672749284447 2212 2 -0.08143113455376394 2212 3 0.26898918318595477 2212 4 0.35436570696699476 2212 5 0.33328876609894564 2212 6 0.25806707898184555 2212 7 0.4384082827496485 2212 8 0.41661985851220606 2212 9 0.5325493251170741 2212 10 0.06523199595028473 2212 11 0.20905749150620778 2212 12 0.32896444001982417 2212 13 -3.759732155828033E-4 2212 14 0.10890732568460493 2212 15 0.09558882918680922 2212 16 -0.3229011107710704 2212 17 0.03643512868757881 2212 18 0.05973503197325414 2212 19 0.028654614200462447 2212 20 0.42029095403365346 2212 21 0.4611152967894725 2212 22 0.3256094188529559 2212 23 0.6503914042642336 2212 24 0.2454890980582789 2212 25 -0.16513452660353067 2213 1 -0.0371500887414129 2213 2 -0.24283093650659993 2213 3 -0.7979254262466178 2213 4 -1.3565487642931373 2213 5 -2.0850075677256683 2213 6 -0.8180912666334805 2213 7 -0.6047107297779846 2213 8 -0.5036437054155769 2213 9 -1.0647073762462256 2213 10 -0.8935304395692469 2213 11 -0.906672864978681 2213 12 -1.1516378766714084 2213 13 -1.0232498448073315 2213 14 -8.082330238795866 2213 15 -3.7477535621175413 2213 16 -2.3213656695404126 2213 17 -1.6004033707597287 2213 18 -6.0576942697953555 2213 19 -3.759009465571097 2213 20 -5.722562652267008 2213 21 -2.5981117435568777 2213 22 -1.9172125229303916 2213 23 -5.037205892161701 2213 24 -2.6496063560684746 2213 25 -2.1282908456897474 2214 1 0.19746822739367076 2214 2 0.08653263056656968 2214 3 -1.546090294914535 2214 4 -2.1808481310848826 2214 5 -0.4768403538419932 2214 6 -0.5383128895227856 2214 7 -0.5594715177704792 2214 8 -0.33937746479644204 2214 9 -5.41663778196855 2214 10 -1.6498801112390389 2214 11 -1.2396187491250217 2214 12 -1.1971892955269396 2214 13 -1.4144626145071442 2214 14 -2.5371437941429598 2214 15 -2.080322745982457 2214 16 -1.3323925808912591 2214 17 -1.3877699866133923 2214 18 -0.8411575671687146 2214 19 -4.592749791556088 2214 20 -2.0522135082707607 2214 21 -3.88898445697151 2214 22 -6.185346903793427 2214 23 -6.328949266927182 2214 24 -5.409126466293614 2214 25 -3.1104144385602943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27807.0 3 18.0 4 500.0 5 3260.0 6 4071.0 7 3533.0 8 2612.0 9 2046.0 10 1857.0 11 1717.0 12 1591.0 13 1615.0 14 1641.0 15 1588.0 16 1698.0 17 1804.0 18 2026.0 19 2341.0 20 3089.0 21 3984.0 22 5147.0 23 6197.0 24 7511.0 25 8943.0 26 10186.0 27 11304.0 28 12995.0 29 15701.0 30 19535.0 31 24481.0 32 31570.0 33 40854.0 34 53196.0 35 70199.0 36 94704.0 37 140527.0 38 222044.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.156260488305875 19.671703425399166 9.443062804716787 29.728973281578174 2 13.711661822785052 21.34658328552667 39.38455046724098 25.5572044244473 3 18.720286740730295 24.299593807670835 28.883984501398025 28.096134950200845 4 12.007984618703638 17.442527577516945 37.41533530420022 33.134152499579194 5 13.18345809767175 38.50370500305787 35.144312595114044 13.168524304156334 6 32.000295005953646 38.30521458709278 17.244882300951275 12.449608106002302 7 28.432527151072506 31.719504608677894 21.360664067508463 18.48730417274113 8 24.961087530794572 38.48101866967439 19.508236621056067 17.049657178474963 9 25.746719208406994 16.006154934811594 19.721911876625853 38.525213980155556 10 14.278908202701404 30.404855276771396 33.92341395832815 21.392822562199047 11 34.55317646250233 23.14123233307145 23.717499783736766 18.58809142068946 12 22.803447932615054 25.357875876321888 31.657794820990958 20.180881370072097 13 28.838879856664125 22.283301654714112 25.580406023519597 23.29741246510217 14 20.41380438920096 22.68892217095519 27.537510509346053 29.359762930497794 15 22.601738157704514 31.511396143062655 23.075027077245398 22.811838621987434 16 21.76221247086344 29.208255764210406 27.500767297371283 21.528764467554875 17 21.304930551358577 29.333740983408408 27.687142787733997 21.67418567749902 18 21.196274307654022 28.762487112943937 29.32691054309313 20.71432803630891 19 22.99227732885876 27.579546356764418 27.41743780802698 22.010738506349846 20 22.771731659801585 28.19735887870845 28.083362168260095 20.947547293229878 21 22.440575856360596 27.295468253601474 27.335046824767655 22.92890906527028 22 22.27713972878046 28.582211941812457 27.362861598403587 21.77778673100349 23 21.940366776791343 28.311087200731848 27.590141866494765 22.158404155982044 24 22.58626430727264 27.56819913519432 28.415585462069064 21.429951095463977 25 22.34148445535685 27.871220487929737 27.907955046380344 21.879340010333078 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 213.0 2 234.0 3 255.0 4 255.0 5 255.0 6 410.0 7 565.0 8 565.0 9 565.0 10 1019.0 11 1473.0 12 1473.0 13 1473.0 14 2488.0 15 3503.0 16 3503.0 17 3503.0 18 6308.5 19 9114.0 20 9114.0 21 9114.0 22 14688.5 23 20263.0 24 20263.0 25 20263.0 26 29069.0 27 37875.0 28 37875.0 29 37875.0 30 50435.5 31 62996.0 32 62996.0 33 62996.0 34 72694.0 35 82392.0 36 82392.0 37 82392.0 38 89635.5 39 96879.0 40 96879.0 41 96879.0 42 102238.0 43 107597.0 44 107597.0 45 107597.0 46 110850.5 47 114104.0 48 114104.0 49 114104.0 50 109555.5 51 105007.0 52 105007.0 53 105007.0 54 92461.5 55 79916.0 56 79916.0 57 79916.0 58 67672.5 59 55429.0 60 55429.0 61 55429.0 62 44984.0 63 34539.0 64 34539.0 65 34539.0 66 26467.0 67 18395.0 68 18395.0 69 18395.0 70 13348.5 71 8302.0 72 8302.0 73 8302.0 74 5821.0 75 3340.0 76 3340.0 77 3340.0 78 2286.0 79 1232.0 80 1232.0 81 1232.0 82 797.0 83 362.0 84 362.0 85 362.0 86 238.5 87 115.0 88 115.0 89 115.0 90 69.0 91 23.0 92 23.0 93 23.0 94 13.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08188251577216041 2 0.025595692339778077 3 0.024055210856365507 4 0.031994615424722594 5 0.01990776070871628 6 0.38286889791584705 7 0.18580576661468529 8 0.1916121968213942 9 0.1944561626369251 10 0.012916344745536159 11 0.0015404814834125693 12 0.002843965815530897 13 0.0 14 0.07050665251003684 15 0.0011849857564712072 16 0.002488470088589535 17 0.0036734558450607423 18 0.002606968664236656 19 0.0015404814834125693 20 9.479886051769658E-4 21 1.1849857564712073E-4 22 0.0 23 0.0 24 3.5549572694136214E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 843892.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.37682947939761 #Duplication Level Percentage of deduplicated Percentage of total 1 92.60806466757916 79.06582945540933 2 5.630973461799725 9.615093221021766 3 0.914365824245174 2.3419696517510724 4 0.31735190594912893 1.0837799823672243 5 0.1475105527087396 0.6296991652512877 6 0.08682886046688879 0.44479036883831835 7 0.05916601428330188 0.35359846987087473 8 0.04156764912700816 0.28391312731008056 9 0.029977614092483233 0.2303454281915815 >10 0.1409074381036936 2.1864435546123495 >50 0.011992991104540976 0.710761966151924 >100 0.00934114057667111 1.589320318913866 >500 0.0015336199712673855 0.9939203181412153 >1k 4.1825999216383246E-4 0.47053497216912865 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1439 0.17051945035620672 No Hit GTCCTACAGTGGACATTTCTAAATT 1275 0.15108568395007893 No Hit CTGTAGGACGTGGAATATGGCAAGA 1239 0.14681973522678257 No Hit GTCCTACAGTGTGCATTTCTCATTT 976 0.11565460983158982 No Hit GTCCTAAAGTGTGTATTTCTCATTT 863 0.10226427078346519 No Hit GGTATCAACGCAGAGTACTTTTTTT 861 0.10202727363217093 No Hit CTTTAGGACGTGAAATATGGCGAGG 859 0.10179027648087671 No Hit CTGTAGGACCTGGAATATGGCGAGA 854 0.10119778360264109 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1849857564712073E-4 2 0.0 0.0 0.0 0.0 1.1849857564712073E-4 3 0.0 0.0 0.0 0.0 1.1849857564712073E-4 4 0.0 0.0 0.0 0.0 1.1849857564712073E-4 5 0.0 0.0 0.0 0.0 1.1849857564712073E-4 6 0.0 0.0 0.0 0.0 1.1849857564712073E-4 7 0.0 0.0 0.0 0.0 1.1849857564712073E-4 8 0.0 0.0 0.0 1.1849857564712073E-4 1.1849857564712073E-4 9 0.0 0.0 0.0 1.1849857564712073E-4 1.1849857564712073E-4 10 0.0 0.0 0.0 1.1849857564712073E-4 1.1849857564712073E-4 11 0.0 0.0 0.0 1.1849857564712073E-4 1.1849857564712073E-4 12 0.0 1.1849857564712073E-4 0.0 1.1849857564712073E-4 1.1849857564712073E-4 13 0.0 1.1849857564712073E-4 0.0 1.1849857564712073E-4 2.3699715129424145E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGG 250 0.0 13.2654085 19 GATATAC 230 0.0 13.263965 1 TAGGACC 360 0.0 13.239359 4 TCCAACG 80 2.0555399E-6 13.028526 18 GCCTATA 75 1.4242123E-5 12.7113 1 GTCTTAT 70 1.05454295E-4 12.257325 1 TGTAGGA 855 0.0 12.159585 2 CTACACT 200 0.0 11.915422 4 GGACCTG 465 0.0 11.684104 6 GCCATAT 245 0.0 11.673641 1 TTAATAC 90 7.153447E-6 11.653414 3 GTAGCCC 150 1.6552804E-10 11.441533 3 TTAGGAC 395 0.0 11.34498 3 ACTGTTC 310 0.0 11.340802 8 CTGTAGG 850 0.0 11.3280115 1 TGGCGAG 645 0.0 11.311589 18 GTAGGAC 825 0.0 11.094821 3 CGTGGAA 275 0.0 11.056584 9 GACGTGG 275 0.0 11.054615 7 TAGGACG 630 0.0 11.045408 4 >>END_MODULE