FastQCFastQC Report
Thu 26 May 2016
SRR1926599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739206
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC500.0024387915.9996671
GTATAGA604.5720197E-415.5552311
ACTGTTC1006.9558155E-913.6507318
TTTAGGG700.001240614213.6346062
AGGACAT1600.013.0046165
AATAGTA653.7277266E-412.8045455
AGGACCT2800.012.6330555
GATATAC957.94448E-512.6313161
TCCTACA4750.012.62995152
ATAGGAC704.6077862E-412.4865743
TTAAACA1202.4472683E-812.4768244
TTACACC600.002420806312.4768244
GTATATA1401.387125E-712.3806941
GTCCTAC4500.012.1478951
TAGCCAG600.00297671212.1376415
TGTAGGA3450.011.8561792
GTATTGG800.00400048611.6664241
CCTAGAC758.2300283E-411.6541373
TAAGGAT650.00440699911.5170684
TAGGACA1503.8235157E-911.4073824
Produced by FastQC (version 0.11.5)