FastQCFastQC Report
Thu 26 May 2016
SRR1926599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739206
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15120.20454379428738403No Hit
GTCCTACAGTGGACATTTCTAAATT11110.15029639910931458No Hit
CTGTAGGACGTGGAATATGGCAAGA10830.14650855106695562No Hit
GTCCTAAAGTGTGTATTTCTCATTT9390.1270281897062524No Hit
GGTATCAACGCAGAGTACTTTTTTT9290.12567538683398133No Hit
CTTTAGGACGTGAAATATGGCGAGG8970.12134641764271395No Hit
GTCCTACAGTGTGCATTTCTCATTT7900.10687142690941362No Hit
TATCAACGCAGAGTACTTTTTTTTT7830.10592446489882387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2650.014.7600974
TAATACC655.234035E-513.2093174
CCTTATA603.9290322E-412.7261532
GTAGGAC7400.012.3788283
GCATAAG550.002969148812.1427251
GTGCTAG959.85061E-712.0514271
GTATAAG959.85061E-712.0514271
TGTAGGA7550.012.009782
AGGACGT6100.011.885985
GGACGTG6100.011.5731916
GGCGAGG3700.011.51880419
ATACTTC751.991581E-411.4480756
AGGACCT4850.011.4087355
CTGTAGG7650.011.3490841
TAGGACG6800.011.2236034
AAGATTG600.00568959311.1300735
GTGTATA1552.8921932E-1011.0795371
CTAATAC951.2925768E-511.0486473
GTTCTAA951.2945318E-511.0471411
GAGTCCC700.001489879410.8570669
Produced by FastQC (version 0.11.5)